the liquid corresponds to the velocity at the edge or vane tip of the impeller. The faster the impeller revolves or the bigger the impeller is‚ the higher will the velocity of the liquid energy transferred to the liquid be. This is described by the Affinity Laws. Pressure and Head If the discharge of a centrifugal pump is pointed straight up into the air the fluid will pumped to a certain height - or head - called the shut off head. This maximum head is mainly determined by the outside diameter
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procedure for GCR protein (PA1510A Life Technologies at 1:1000) that used DAB to stain the collected tissue to locate GCR proteins. Primary antibody is created so there is a high affinity for the protein in the experiment. For this study‚ the primary antibody is selective for GCR protein. The secondary antibody has a high affinity for the first‚ and is also bound to HRP. In the presence of GRCs‚ the primary antibody will bind to the GRCs. The secondary antibody will bind to the primary and then DAB will
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Specificity: Induced Fit • Conformational changes may occur upon ligand binding (Daniel Koshland in 1958). – This adaptation is called the induced fit. – Induced fit allows for tighter binding of the ligand – Induced fit can increase/change the affinity of the protein for a second ligand • Both the ligand and the protein can change their conformations + 2 Myoglobin: Function • Metabolism is much more efficient when under aerobic conditions • Glycolysis of 1glucose results in 2 ATP. • When under
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First‚ the amount of total protein removed by the Affinity Chromatography is difficult to quantify because there appears to be an error in the analysis data for 65% cut precipitate. The protein from the previous purification step limits the amount of protein that can be recovered by the subsequent purification step. In this case‚ the total protein recovered by 65% cut was 159 mg‚ but Affinity Chromatography recovered 350 mg of protein. Purification steps cannot gain protein
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groups (wolfe‚ 2000) Oxygenated/deoxygenated oxygenated • 02 bound • Bright red • Subunit is in relaxed form • Higher affinity for 02 • Affinity for oxygen is cooperative‚ which means that the affinity for 02 increases with each bound molecule. ( Ahern & Rajagopal‚ 2013) Deoxygenated • 02 not bound • Dark red color • In tense form which has lower affinity for oxygen. • When 02 binds to deoxygenated hemoglobin‚ hemoglobin transitions from T state to R state. This occurs when
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Specificity of Protein-Ligand Binding 1). One can conclude that Orange G. has the lowest affinity for the albumin. In the experiment the concentration of Orange G that binds to protein was a lot less than either Ponceau S. or Bromophenol blue. The ligand with the highest affinity to BSA was a little more difficult to decipher. The experiment shows that all of the 2 µL of both Ponceau S. and Bromophenol blue bind to the BSA. However‚ when 5 µL of the ligand is added the BSA is saturated and cannot
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Quality Circles Quality Circles (QC) or Quality Control Circles (QCC) : History * Pioneered by Japanese. * Japanese nomenclature: Quality Control Circles (QCC)‚ generally now known as Quality Circles (QC) or some call it as Small Group Activity (SGA). * 1962: First QC Circle was registered with QC Circle Head Quarters in Japan. * 1974: Lockheed Company‚ USA started Quality Circle movement. * 1977: International Association of Quality Circles (IACC) was formed in USA.
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Business plan The Candy Shop -------“I love Sweet” Executive Summary Business overview We decide to setting up a sweet shop in Sydney CBD. The name of this shop is “I love sweet”. We make this name because we want to provide the best lollies and sweets to people who like sweet. They can get the satisfy product in our shop. Also “I love sweet” is known as our slogan‚ we wish customers enjoy our sweets and love our sweets. Our shop could be a paradise to the lolly fans. The feature product
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MIND MAP PROJECT MANAGEMENT PROFESSIONAL PROJECT MANAGEMENT PROFESSIONAL document issued by the project initiator or sponsor authorizes the existence of a project Project Charter provides the project manager with the authority to apply organizational resources to project activities the document that describes how the project will be executed monitored‚ and controlled any unique and verifiable product‚ result‚or capability to perform a service that is required to be produced to complete a process
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15.2 Born-Haber Cycle Our calculations of enthalpies so far have involved covalent substances. Now we need to look at the enthalpy changes involved in the formation of giant ionic lattices. Lattice enthalpy is defined as either the: ’the enthalpy change that occurs when one mole of a solid ionic crystal is broken into its ions in the gaseous state‚ at standard temperature and pressure. Because all the bonds in the ionic lattice are broken‚ it is an endothermic process‚ ∆H is positive. ’ The
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