Citrate Test: Some bacteria can use citrate as a source of carbon. To test if the unknown bacteria uses citrate as a source of carbon‚ Simmon’s citrate agar was used as the medium on which the bacteria was grown. The Simmon’s citrate agar consists of sodium citrate as the source of carbon‚ ammonium dihydrogen phosphate as the source of nitrogen along with pH indicator such as bromothymol blue. Procedure: The Citratase activity was detected by inoculating the unknown bacteria on the slant surface
Premium Escherichia coli Escherichia coli PH indicator
Flame Test Lab Morgan Chemistry CP 5/30/14 Abstract- In the flame test lab‚ the flame test was performed to excite the electrons in the samples and observe the color of the flame. The flame emits a color because each element has an exactly defined emission spectrum‚ which one can use to identify them. For example‚ NaCl was highlighter yellow‚ Sr(NO3)2 was sun orange‚ CuCl2 was turquoise‚ LiCl was neon red‚ KCl was solar flare yellow‚ and BaCl2 was Voldemort green. Introduction- In Bohr’s
Premium Chemistry Potassium Gas
Background In the Flame test lab we took a simple wire‚ looped it‚ and rinsed it in HCL (Hydrochloric Acid ). Then we dipped the wire in the different samples provided. The samples provided were Sodium‚Potassium‚Calcium‚ Barium‚ Strontium‚ Lithium ‚ and Copper II. Finally we put the wire loop into the flame of the bunsen burner. The flame erupted into many different colors for each specific sample. We then used the colors we recorded to find the two unknown samples given to us. But the question
Premium Oxygen Light Chemistry
urpose: The purpose of this lab is to determine the impact of light on the germination of seeds Research: A seed is the reproductive unit of a plant that contains a mature ovule with an embryo. The seed is made up of the seed coat hard shell over the seed‚ two cotyledons‚ fleshy membranes that store food‚ an epicotyl‚ which forms the primary shoot‚ the radicle‚ the primary root of the seed‚ and the hypocotyl‚ the stem of the epicotyl. When the seed is triggered by water to sprout‚ a process called
Premium Seed Germination Plant morphology
Purpose: To use a set of standardized procedures to test for simple sugars and starch‚ proteins‚ and fats. Hypotheses: A.) B.) C.) Equipment: -Safety goggles -Lab apron -400 mL beaker -Utility stand with ring clamp -Hot plate -Thermometer -10 mL graduated cylinder -Test-tube racks -8 to 16 test tubes -Test tube holder -Medicine dropper -Rubber stoppers -Test-tube brush -Wax pencil -Distilled water
Premium Nutrition Cholesterol Starch
My partner and I were given unknown number 3 in the laboratory. After performing various tests over the course of a few weeks on our unknown‚ we came to the conclusion that our unknown organism is Klebsiella pneumoniae (K. pneumoniae). K. pneumoniae is a gram negative bacillus shaped microorganism. We observed that K. pneumoniae is a nonmotile organism. We performed multiple tests on our unknown culture‚ therefore we are very confident that it is correctly identified. We identified that K. pneumoniae
Premium Bacteria Microbiology Gram staining
During the lab experiment six different test were conducted. Each test consists of chemicals to be tested using 3 to 10 drops of the additive in order to observe a change. With any experiment you compose a hypothesis; where you make an educated guess. The hypothesis will primarily be‚ which base chemical will produce the greatest change based on the additive. Experiment 6.1 (Starch) when adding the three drops of Iodine to each tube observe the color change. When performing this test the chemical
Premium Chemistry Hydrochloric acid Chemical reaction
Heat Treatment and Qualitative Metallographic Analysis Lab ME 3228: Mechanics & Materials Laboratory Summary Comparing a material’s initial treatment and the microstructures that are formed from different processes‚ an engineer can accurately understand why certain treated materials of the same family can be stronger than another. In this experiment annealed and cold-worked specimens of AISI 1018 STEEL are used‚ along with a Hardness tester‚ a grinding and polishing process‚ and a micrograph
Premium Temperature Heat Oxygen
The presence of carbohydrates in a solution can be determined by a qualitative test of Molisch test. Molisch test is a general‚ sensitive chemical test and positive for all kinds of carbohydrates which in free form or in combined form. The test is based on the dehydration of the carbohydrate by concentrated sulfuric acid to produce an aldehyde which condenses with two molecules of phenol‚ resulting in a coloured compound. Molisch reagent is a solution of α-napthol in 95% ethanol. The aldehydes produced
Premium Glucose Carbohydrate Nutrition
Qualitative Analysis Qualitative is where the results give a colour that you have to match up to a colour chart‚ or have you have done to see if it is positive or negative with a description of what a positive reaction should look like. This test is less reliable as some colour or positive reaction look similar to each other or the same but by adding different chemicals. An example of qualitative would be a pH test as the result gives you a colour and then you have to match it up to the pH scale
Premium Ion Electric charge Chemistry