Background: Pepsinogen is an inactive protein that is the mucus lining of the stomach. When converted to pepsin‚ the enzyme is used to break down large or undigested protein they has been absorbed by the small intestine. The digestive power of pepsin is greatest at the acidity of normal gastric juice (pH 1.5–2.5). In the intestine the gastric acids are neutralized (pH 7)‚ and pepsin is no longer effective. "Pepsin (biochemistry)." Encyclopedia Britannica Online. Encyclopedia Britannica‚ n.d. Web
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supernatant liquid and denser slurry having a higher concentration of solids. This is usually accomplished by allowing the particles to settle through the force of gravity‚ mechanically using centrifugal force‚ or electrostatically using an electric current. Continuous sedimentation tanks are usually used in wastewater treatment facilities to separate suspended particles from wastewater. This experiment aims to determine the effect of initial concentration and initial height of the slurry on its settling
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is one of the most widely used methods in clinical immunology assays to detect the presence and absence of certain antigens or antibodies and also to quantify them when necessary. Quantification can be done in a range of microgram (µg) to nanogram (ng). The ELISA procedure takes advantage of the fact that most proteins will bind firmly to the surface of different kinds of plastic (polystyrene or polyvinyl chloride)‚ usually by hydrophobic interaction. The steps of the ELISA procedure are simple
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If pH > pI‚ then the protein will have a negative charge and if pH < pI‚ the protein will have a positive charge. Buffer I has a pH >5‚ meaning both proteins carry a negative charge and bind to the DEAE (a positively charged resin). (b) pH = pKa + log10(Base/Acid) [Base = mM of sodium acetate; Acid = mM of acetic acid] = 4.7 + log10 (40/40) = 4.7 In order for the catalase to elute from the column‚ it must have lost its negative charge and stopped binding to the DEAE. Lowering the pH
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(ELECTROPHORESIS OF SERUM PROTEIN) DATE : 10 OCTOBER 2013 PART A SEPARATION OF SERUM PROTEINS USING THE ELECTROPHORESIS METHOD. OBJECTIVES: 1. To understand the analytical methods involved in analyzing serum proteins. 2. To study the serum protein electrophoresis pattern to aid the understanding of the relationship between the structure and the function of proteins. PRINCIPLE OF PRACTICAL: This technique is based on the movement of charged particles such as proteins when placed
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It makes sense to think that quick‚ post-workout protein intake might boost recovery and promote higher performances on subsequent days of training. Indeed‚ some research has suggested that protein intake can help athletes adapt to their workouts more effectively. However‚ such studies have been flawed methodologically‚ and a new investigation reveals that combining protein with carbohydrate after workouts is not a superior strategy‚ compared with taking carbs alone. You’ve heard them: The "experts"
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Experiment No. 3 Determination of Chromium (VI) by Direct Visible Spectrophotometry (External Calibration Method) Group Members: Cabahug‚ Elisha Niña M. Date Performed: November 20 & 22‚ 2012 Mejia‚ Helen Mae N. Date Submitted: November 29‚ 2012 Score: I. Introduction Spectrophotometric measurements with UV or visible light radiation are useful in detecting transition metal ions and highly conjugated organic compounds. In UV and visible light regions‚ energy spaces molecules
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Hand-out 3: Market Concentration Specification Market concentration. Definition “’Market concentration’ is the degree to which the output of an industry is dominated by its largest producers.” In other words‚ how many of the sales in the market are accounted for by the biggest firms in that market. Firm Sales (£m) % Market Share A 56 B 43 C 22 D 12 E 3 F 1 Total 100% Calculate the 3 firm concentration ratio of this market. 3 firm concentration ratio = __________________________________________________________
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What is the Molar Concentration (Solute concentration) of a Potato? ------------------------------------------------- ------------------------------------------------- ------------------------------------------------- Design ------------------------------------------------- ------------------------------------------------- 1. What is the dependent variable for this experiment? ------------------------------------------------- The dependent variable is the concentration (solute) of the
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Concentration of a Solution: Beer’s Law The objective of this experiment is to determine the concentration of an unknown copper sulfate solution. You will be using the Colorimeter. In this device‚ red light from the LED light source will pass through the solution and strike a photocell. A higher concentration of the coloured solution absorbs more light (and transmits less) than a solution of lower concentration. The Colorimeter monitors the light received by the photocell as either an absorbance
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