Expressing and Purifying the Recombinant form of Green Fluorescent Protein (rGFP) from the E.coli strain using Ni2+ agarose affinity chromatography technology Abstract The purpose of this experiment was to express and purify the his6-tagged recombinant form of GFP (rGFP) from the organism E.coli using Ni2+ agarose affinity chromatography. The expression of rGFP was confirmed qualitatively using the UV light and was expressed in the E.coli strain BL21 (DE3) (-- removed HTML --) (-- removed HTML
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Title: The effect of temperature on beet cell membranes. Introduction: 1. Eukaryotic cells are much more intricate than prokaryotic cells. The first thing observed is the assortment of structures in the cytoplasm. The fluid filled region between the nucleus and the plasma membrane. These structures along with the nucleus are the organelles‚ each having a specific function in the cell. The organelles are by membranes divided in proficient sections .The plant cell is made up of a rigid thick wall
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The Plant Cell‚ Vol. 17‚ 1829–1838‚ June 2005‚ www.plantcell.org ª 2005 American Society of Plant Biologists Dual Role for Tomato Heat Shock Protein 21: Protecting Photosystem II from Oxidative Stress and Promoting Color Changes during Fruit Maturation Inbal Neta-Sharir‚a Tal Isaacson‚b Susan Lurie‚c and David Weissa‚1 a Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture‚ Faculty of Agricultural‚ Food‚ and Environmental Quality Sciences‚ Hebrew University of Jerusalem‚ Rehovot
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Ricin is a toxic protein found in castor oil plant seeds. The toxicity of Ricin has been known for centuries and was even used by the KGB government to assassinate Georgi Markov‚ a famous journalist who openly criticized the Soviet Union’s communist regime. The toxicity of ricin is caused by its ability to deactivate ribosomes and halt protein synthesis. Therefore‚ Ricin is categorized as a ribosome inactivating protein (RIP). There are two types of RIPs: type I and type II. Type I RIPs are usually
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2 Production of Protein from Cloned Genes Now that we have covered the basic techniques involved in gene cloning and DNA analysis and examined how these techniques are used in research‚ we can move on to consider how recombinant DNA technology is being applied in biotechnology. This is not a new subject‚ although biotechnology has received far more attention during recent years than it ever has in the past. Biotechnology can be defined as the use of biological processes in industry and technology
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Abstract: The main objective of this Lab‚ is to experiment with stoichiometric measurements‚ since it is one of the most important subjects in chemistry. In addition‚ stoichiometric measurement is widely used in chemistry‚ because it indicates the proportion of mass in which various substances react. By examining one specific example of stoichiometric determination‚ which is: “Stoichiometry and limiting reacting”‚ we found out the different mass proportions in which substance react. In this case
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Title: “The Effect of Substrate Concentration‚ Enzyme Concentration‚ pH and Temperature on Enzyme Activity” Abstract: In the following experiments we will measure precise amounts of potato extract as well as Phenylthiourea‚ combined with or without deionized water and in some instances change the temperature and observe and record the reaction. We will also investigate the different levels of prepared pH on varying samples of the potato extract and the Phenylthiourea and record the results.
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MILK FABRIC ABSTRACT: The milk fabric is new born fabric. The milk protein fabric moistens skin and it is healthy and bacteriostatic - it is the perfect material to produce underwear and socks‚ pants‚ long-sleeve T’s and jackets also. The important ingredients of milk protein fiber are milk casein proteins‚ which can nourish and lubricate the skin. The milk protein contains the natural humectant factor‚ which can capture the moisture and maintain skin’s moisture to make the skin tender and smooth
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A protein standard curve was generated by using the protein standard (1 ampule) measurement at 595 nm. Standard curve was prepared as shown in Table 4. Bovine serum albumin (BSA) was diluted with distilled water. The absorbance of each sample were measured using spectrophotometer at 595nm. Standard protein curve was plotted according to the concentration of protein where the x-axis and y-axis represent protein standard concentration and absorbance (595 nm) respectively. 3.10.2 Protein Determination
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find the concentration (mole/dm3 (M)) of solute in a potato cell by using the process of osmosis and different concentrations of sucrose solution. Background information Osmosis is diffusion of water across a partially permeable membrane. It moves from a solution with less solute concentration (high water potential) to a solution with more solute concentration (low water potential). The one with a high water concentration is called a hypotonic solution and the low water concentration is called
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