B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification. B. Introduction I. Fast Protein Liquid Chromatography
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Proteins are polymeric chains that are built from monomers called amino acids. All structural and functional properties of proteins derive from the chemical properties of the polypeptide chain. There are four levels of protein structural organization: primary‚ secondary‚ tertiary‚ and quaternary. Primary structure is defined as the linear sequence of amino acids in a polypeptide chain. The secondary structure refers to certain regular geometric figures of the chain. Tertiary structure results from
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Lab 2: Internet Resources for Protein Biochemistry Part 1-1: A) Protein Accession Number = P00939 Name of Protein: Full Name = Triosephosphate isomerase; Short Name=TIM Organism where the protein is found: Oryctolagus cuniculus = Rabbit Nuber of amino acids in the protein: AA = 248 B) * Amino Acid Sequence: >gi|136066|sp|P00939.1|TPIS_RABIT RecName: Full=Triosephosphate isomerase; Short=TIM;
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“Denaturation of Proteins” Denaturation is a process in which proteins or nucleic acids lose the tertiary structure and secondary structure which is present in their native state‚ by application of some external stress or compound such as a strong acid or base‚ a concentrated inorganic salt‚ an organic solvent (e.g.‚ alcohol or chloroform)‚ or heat. If proteins in a living cell are denatured‚ this results in disruption of cell activity and possibly cell death. Denatured proteins can exhibit
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LAB #11A: Quantification of a Mutagen – Standard Ames Test Sarah R. Villarreal Rogers 1130 INTRODUCTION. In the experiment Lab 6A‚ Diet Coke was determined a mutagen by using a test called the Spot-Overlay Ames Test. The Spot-Overlay Test was designed and named after Dr. Bruce Ames‚ a Scientist and professor of Biochemistry and Molecular Biology at an array of colleges (Ames 2012)‚ the test was designed to determine whether or not something is mutagenic. After the Spot-Overlay Ames Test
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Identifying sites of protein synthesis in Chlamydomonas using erythromycin and cyclohexamide as protein synthesis inhibitors. October 16‚ 2009 Introduction: In living cells‚ prokaryotic or eukaryotic‚ the synthesis (construction) of proteins is accomplished by similar machinery. Amino acids‚ ribosomes‚ messenger RNA (mRNA)‚ and transfer RNA (tRNA)‚ are all necessary for the building of functional proteins in a cell. Ribosomes are the site of protein synthesis in a cell‚ and there are two
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determine the Keq of the formation of Fe(SCN)2+ through the use of UV-Vis spectrophotometry. The solutions used in the study were allowed to equilibrate days before calibration in a UV-Vis spectrophotometer which determined the absorbance and thus the molar absorptivity of Fe(SCN)2‚ which was found to be 1.942 x 10-3 M-1cm-1. Given the molar absorptivity and absorbance‚ Beer-Lambert’s Law gave the equilibrium concentrations for the product and reactants and from that‚ the Keq of the formation of Fe(SCN)2+
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Protein function • Chapter 5.1 • Myoglobin: structure‚ O2-binding • Hemoglobin: structure‚ cooperativity in O2binding‚ Hill constant‚ allosteric interactions‚ Bohr Effect‚ BPG-binding and effect • Abnormal Hemoglobins Functions of Proteins Fibrous proteins: collagen‚ keratin‚ silk - give tensile strength‚ shelter‚ protection Globular proteins: • Storage of ions and molecules – myoglobin‚ ferritin • Transport of ions and molecules – hemoglobin‚ serotonin transporter • Defense against pathogens –
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accepted the origin of all life on Earth. In the article‚ “The Proof is in the Proteins: Tests Supports Universal Common Ancestor for All Life”‚ by Katherine Harmon‚ a scientist by the name of Douglas Theobold carried out statistical analysis on 23 preserved proteins that were preserved over three major domains of life. The result of these analyses supported Darwin’s evolutionary theory‚ by demonstrating that the protein sequences of life today came from a common universal ancestor rather than separate
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Protein Article Research SCI/241 August 1‚ 2013 Dr. Theodore Keneklis Protein Article Research Proteins are made up of amino acids‚ and in our bodies we have proteins that “are part of every cell‚ tissue‚ and organ in our bodies” (Centers for Disease Control and Prevention: Protein‚ http://www.cdc.gov/nutrition/everyone/basics/protein.html). There are complete proteins which are made up from animals. These kinds of proteins are found in meat‚ poultry‚ fish and even cheese. A complete
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