Roy Levin Bio 11 Lab Dr.Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to
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urpose: The purpose of this lab is to determine the impact of light on the germination of seeds Research: A seed is the reproductive unit of a plant that contains a mature ovule with an embryo. The seed is made up of the seed coat hard shell over the seed‚ two cotyledons‚ fleshy membranes that store food‚ an epicotyl‚ which forms the primary shoot‚ the radicle‚ the primary root of the seed‚ and the hypocotyl‚ the stem of the epicotyl. When the seed is triggered by water to sprout‚ a process called
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anticipated. The Tap Water allowed the radish seeds to germinate with in the first 48 hours. In the petri dish labeled tap water‚ 10 out of 10 seeds sprouted into plant growth as seen in Figure 5.1. The radish plant growth within the tap water petri dish grew so well it started to lift the lid off the dish. The results from the Acid Rain (50% vinegar solution) did not allow any radish seeds to germinate. There were never signs of seed germination while observing the seeds over a three week period. In the
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Wave Nature of Light Objective: The purpose of this lab is to investigate interference‚ otherwise known as the diffraction of light. A beam of light acts a wave‚ and we are able to use equations so calculate the wavelength of the light used. The diffraction of a straight edge demonstrates that light waves bend around straight edges‚ allowing light to enter an area of shadow. When waves are superposed‚ they reinforce each other when crests are in phase and cancel out when they are not in phase
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Laboratory Report The Plasma Membrane I. Introduction The Plasma membrane is the edge of life‚ the boundary that separates the cell from its surroundings. It controls the traffic of materials in and out of the cell. (Reece‚ 2011). It is incredibly thin that is very vital in maintaining the integrity of the cell. Not only does the plasma membrane bind the other organelles‚ it also forms a dynamic structure which gives them their remarkable activity and selectivity. (Hickman‚ 2008). Diffusion
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PEES 4200W- Physiology of Exercise Lab #2: Metabolism and Energy Expenditure 1. The Respiratory exchange ratio is the ratio of the volume of carbon dioxide produced and oxygen consumed. The ratio indicates the energy that the subject is expending for indirect calorimetry‚ how efficient the subject ’s body is at utilizing the oxygen inhaled‚ as well as the main substrate being used for energy during varying intensities of exercise. During rest the volume of carbon dioxide was 0.73L/min‚ the volume
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LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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chemicals before use. Abide by specific warnings and directions. 3. Collect all materials needed for a procedure before proceeding. 4. Perform reactions under the hood when directed. Chemicals may be weighed and prepared at balance or lab tables‚ but tests should be carried out under the hood. 5.Acids and caustic chemicals are stored in the hood. Please do not take these chemicals from the hood. Procedure: PART 1: Metathetical reactions Precipitation reactions A1. Add a
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Organic Chemistry II Lab 9 Fermentation of a Carbohydrate: Ethanol from Sucrose * Introduction Ethanol is one of the oldest alcohols and also the least toxic one. Industrially‚ ethanol is made most economically by hydration of ethylene. However‚ ethanol that is intended for human consumption must‚ by law‚ be prepared by fermentation. By either method‚ ethanol‚ of course‚ has the same formula‚ structure‚ and properties. The fermentation takes place with the assistance of enzymes from yeast
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Dulag National High School Dulag‚ Leyte Pesticide From Radish ( Raphanus Sativus ) Extract A research proposal Submitted to: Glendale B. Lamiseria In partial fulfillment of the Subject Entitled Research and Statistics By: Julie Glynes P. Lumpas II-SSC October ‚ 2012 Rationale of the Study Radish is an annual herb widely grown for its edible fleshy‚ hot tasting roots maybe red‚ white or purple. Radish is a coarse annual crop plant. Roots are fleshy‚ pungent and variable
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