For the completion of this experiment the procedures were guided with the Rainbow Transformation1 lab manual. An Escherichia coli bacterial reference plate was used to obtain colonies which were resuspended into a CaCl2 solution that was previously kept on an ice bath. The rainbow transformation mixture containing the plasmid DNA was then added to half of the E. coli cells. These cells were later placed into a water bath set to 42ºC and “heat shocked” to promote the entrance of DNA into the cells
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User 0..* Friend 1 UserID (key) 0..* FriendID(key) 1 UserID1 UserID2 Date 1 Password Email Address FName LName DOB Age Location Weight Height Ftiness Goals Event EventID(key) CreatorID Event title Date Start time End time Location createUser(userID):User setPassword() changePassword setEmail();; changeEmail setFName setLName getEmail() setHeight(); setFitnessGoals(); 0..* Post CreateEvent(title‚date‚starttime‚endtime‚ loation); getUserID(); getEventTitle(); getEventDate(); getEventStartTime();
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imagine living in a neighborhood or even going to school near a health hazard? Rainbow Environmental Services‚ a trash disposal company located in Huntington Beach‚ has had the contract as the city’s trash hauler since the late 1950s. However‚ nearby residents‚ including two of the Ocean View School District’s schools‚ have complained about their health due to the open dump and their demolition debris. I feel that Rainbow is polluting both of the OVSD schools and the homes of nearby residents. To
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The White Rainbow rac·ism ˈrāˌsizəm/ noun noun: racism 1.1. the belief that all members of each race possess characteristics or abilities specific to that race‚ esp. so as to distinguish it as inferior or superior to another race or races.(Google deffinition) Thesis- Racism is the true underlining cause of the death of Tom Robinson‚ and the reason he was incarserated in the first place. Introduction- Tom Robinson was a black man accused of raping
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dance in for colored girls who have considered suicide when the rainbow is enuf‚ and why this is so important in her writing. Ntozake Shange portrays multiple themes in her choreopoem for colored girls who have considered suicide when the rainbow is enuf in her use of music and dance-like movements. Her representation through music‚ dance and the language of her seven actresses represented by the use of seven colors of the rainbow is representative of the colors of her characters and her multiple
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Steve McGinnis Dr. Tracey K. Parker English 101 10/15/2013 Doomsday:Is the Hype worth it? When most people think of the end of the world they imagine situations that would test an individuals resolve and ability to overcome catastrophic events such as a astroid or comet impact or aliens invading our planet to the dreaded " zombie apocalypse". Realistically there are events that could happen and being prepared or having a plan could save your life. How you do so will determine if you survive
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coming. To better understand how each event correlates with each other‚ I will give my interpretations of each central message and summarize what the events all had in common. Episode 1 In Matthew 24: 1-35‚ the central message is the signs of the end times. We see specific and broad signs as the time of the second coming of Christ draws near. Jesus shows his love for his disciples by warning them of these signs. He also encourages them to “be not troubled: for all these things must come to pass
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their team and Coach Taylor has a strong belief in him. Usually the death crawl would just be 10m long but Brock mange to reach the end zone of the field carrying a guy who weighs a hundred and sixty pounds. It showed that nothing is impossible without the presence of God. Coach Taylor’s continuous encouragement led to an unbelievable victory of the team. At the end of the death crawl Brock left his whole team jaw-dropped. And that was the start where Coach Taylor changed the whole philosophy of
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the call for man coverage‚ he is locked on his man and trails him all the way down the field to the end zone. He watches as the ball flies on a direct path towards the receiver’s hands‚ so he leaps into the air and he feels his fingers wrap around the ball before it got to its intended target. As soon as the bottom of his cleats touched the turf‚ he was off‚ sprinting down the field towards the end zone‚ he finds a gap in the red and white uniforms approaching him and he was gone‚ shedding tackle
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Personal Narrative Yet another Thanksgiving‚ and I was stuck in my uncle’s basement watching the Redskins face off the Dallas Cowboys. Michael Jordan‚ Serena Williams‚ and various other famous athletes all grinned at me from their respective Wheaties boxes‚ seemingly mocking my pain. My uncle collected them and‚ for some reason‚ had decided the bright orange boxes would be a great decorative addition to the basement wall behind the television. I remember once‚ when I was younger‚ I was searching
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