Determining the Concentration of an Unknown Protein Solution with the use of the Bradford Method Paul Alcala‚ Andrea Basa‚ Melissa Caagbay‚ Frances Cayomba Department of Chemistry‚ University of Santo Tomas‚ Manila‚ Philippines Abstract The Bradford method used to determine the protein content of a certain solution (Menguito‚ 2010) and involves the acidic Coomassie Brilliant Blue G-250 as a coloring reagent. [1] The dye is originally pinkish-brown in color when it is in its acidic state
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be adjusted by com-pounding. However‚ in- or online monitoring of the dispersion quality achieved in such compounding processes is a challenging task. Here‚ THz technology can provide a valuable contribution. KEYWORDS: - THz technology‚ THz spectroscopy‚ THz sciences. I. INTRODUCTION The term ‘T-rays’ was coined in the early 1990’s by Bell Labs to describe the spectrum in the Terahertz range (1 THz = 1012). [1] The terahertz region of the spectrum lies on the border of where electronics and
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Synthesis of benzilic acid from benzoin utilizing a multi-step reaction. Names: Arian Karim TA Name: Sayantan Das Lab Day & Time: Thursday 7:30-11:40 Lab Section #: Abstract The main purpose of this experiment was to synthesize benzilic acid from benzoin. This requires a multistep synthesis with benzyl as an intermediate product. The first step required HNO3 as an oxidizing agent and the second step required KOH and HCl. The percentage yields of benzil and benzilic acid were 59.5%
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The Basics of NMR Chapter 1 INTRODUCTION [pic] NMR Spectroscopy Units Review [pic] NMR Nuclear magnetic resonance‚ or NMR as it is abbreviated by scientists‚ is a phenomenon which occurs when the nuclei of certain atoms are immersed in a static magnetic field and exposed to a second oscillating magnetic field. Some nuclei experience this phenomenon‚ and others do not‚ dependent upon whether they possess a property called spin. You will learn about spin
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5005/jp-journals-10006-1177 Judith Angelitta Noronha et al NURSING PRACTICE Anemia in Pregnancy—Consequences and Challenges: A Review of Literature Judith Angelitta Noronha‚ Esra Al Khasawneh‚ Vidya Seshan‚ Shanthi Ramasubramaniam‚ Savithri Raman ABSTRACT Anemia during pregnancy is a global public health challenge facing the world today‚ especially in the developing countries. Anemia in pregnancy is an important contributor to maternal mortality/morbidity as well as to the low birth
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2a: Infrared Spectroscopy and Analgesic Drug Identification Abstract: To be able to understand how organic soluble compounds could be isolated from non-organic components‚ a chromatographic separation process was used. The objective of the analgesic drug identification is to isolate the organic compounds/ active ingredients in an unknown drug. The identity of the drug was determined through melting point‚ percent recover‚ and Rf values. The comparable data for acetaminophen from the manufacturer
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Among them were dehydration‚ isolation‚ drying‚ and distillation. We used all of these techniques to get the product we were looking for. In addition to these experimental techniques we also verified our product via spectroscopy which is a new technique. Using IR spectroscopy we were able to specifically analyze the functional groups on our product. Results and Discussions: Reaction Equation: Theoretical Yields: Based on limiting moles of product -Based on C7H14O 24 mL C7H14O
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cloves using steam distillation‚ perform infrared spectroscopy on the sample‚ and analyze the IR to identify the major constituent in the clove oil. The first day the clove oil was removed from the ground cloves into an aqueous solution then removed from the water using DCM. The sample was left in a sealed container for one week and then isolated by drying the sample and removing the DCM. After the clove oil was isolated it was analyzed using IR spectroscopy. Experimental Isolation of Clove Oil: The
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simple organic chemistry experiment is described that investigates the kinetics of the reaction between 1-bromobutane (BuBr) and iodide (I−) as followed by observing the disappearance of BuBr and the appearance of 1-iodobutane (BuI) using 1H NMR spectroscopy. In small groups of three to four‚ students acquire data to examine the concentrationdependence of both the organic substrate and the nucleophile under pseudofirst-order and second-order conditions. After
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Identification of Unknown Organic Compound by Melting Point (M.P)‚ Boiling Point (B.P) and Infrared Spectroscopy Methods and Background The main objective of this lab is to identify the given unknown organic compounds with various methods. Identification of an unknown compound is important to perform through the process of melting point (M.P)‚ boiling point (B.P) and Infrared spectroscopy (I.R). Index of Hydrogen deficiency (IHD) and elemental analysis makes an experiment more efficient in
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