Chapter 3 Centrifugation Biochemistry and Molecular Biology (BMB) 3.1 Introduction 3.2 Basic Principle of sedimentation 3.3 Types‚ care and safety of centrifuges 3.4 Preparative centrifugation 3.5 Analytical centrifugation Analytical Biochemistry (AB) 3.4.3 Ultracentrifugation Koolman‚ Color Atlas of Biochemistry‚ 2nd edition 1 General Steps in Biochemical Separation 2 Separation of Macromolecules Chromatography‚ precipitation Electrophoresis‚ ultracentrifugation 3
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Introduction In my report I will discuss what I did as an experiment and what I hope for it to achieve. Firstly I carried out an experiment to assay the effectiveness that a range of disinfectants have on the growth of ecoli and whether or not it can prevent it from growing. From the experiment i should be able to see that some disinfectants have a greater effect than others do. From this I shall then draw a conclusion and evaluation on what was the most effective‚ and could there have been any
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Investigation - Solution Colour and Ion Concentration Aim: To investigate whether solution colour can be used to reliably determine the concentration of coloured ions in a solution. Hypothesis: the concentration of permanganate ions in the solution is inversely proportional to the percentage transmission of light through the solution. Dependent variable: concentration of permanganate ions. Independent variable: percentage transmission of light through the solution. Equipment: 20 ml
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Finding out the accurate concentrain of sulphuric acid Aim The point of this investigation is to find out the concentration of the sulphuric acid in my experiment. I will do this by titrating the sulphuric acid with sodium carbonate. H2SO4 + Na2CO3 à Na2SO4 + H2O + CO2 Acid + Alkali à Salt + Water + Carbon dioxide Ratio of sulphuric acid to sodium carbonate: H2SO4 : Na2CO3 1 : 1 Concentration of sulphuric acid: H2SO4 is approximately 0.05 - 0.15 moldm-3 The average concentration of the
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Aim • To calculate the SHC of water • To see how accurately I can measure a known quantity Research Question • Will the experiment carried out give us a good approximation of the SHC of water? Hypothesis • The result we will get as the SHC of water will not be exactly the same as the literature value‚ but will be a very close figure to the literature value of the SHC of water as there will be some limitations to our experiment. Variables Independent: temperature of water Dependent:
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Problem: How do protease in some fruit juices help softening meat? Hypothesis: The protease in some fresh juices digests collagen‚ which gives meat a tough texture by holding meat fibres together. Design of investigation: In order to test the hypothesis‚ the presence of protease in those fresh juices was tested at first. Then the amount of them was needed to be compared for finding their difference in effectiveness in tenderizing meat. Control experiments were set up for proving protease was
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CO2 Lab Report Intro Alka-Seltzer tablets are used to treat cold/flu symptoms as it produces CO2 when dissolved in water. The goal of this lab is to calculate the amount of NaHCO3 is needed to produce enough CO2 gas to fill the room. Experimental One Alka-Seltzer tablet was weighed on the analytical balance. Then a piece was measured out to be between 0.21 and 0.28 grams. 500mL of distilled water was added to a 600mL beaker. 80mL of water was mixed with 20mL of 6M HCl in a 150mL beaker. A
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In the first experiment‚ cyclohexanol was oxidized to form cyclohexanone. This was accomplished by pouring 2.0 ml of cyclohexanol and 1.0 ml of acetic acid into a 250 ml Erlenmeyer flask. Under a fume hood‚ slowly add 30 ml of bleach‚ and oxidizing agent‚ to the mixture and stir for 20 minutes. Acetic acid will react with bleach to make it a better oxidizing agent and form hopochlorous acid. Hypochlorous acid will then oxidize cyclohexanol. The mixture turned cloudy during the process but clear up
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METHODS In this experiment‚ plasmid lux and a control plasmid (pUC18) will be introduced into E. coli by transformation. There are four basic steps to the procedure; Preparation of competent cells (These steps should be performed by the instructor.) 1. Place a vial of CaCl2 solution and the tube of E. coli in the ice bath. 2. Using a sterile pipet‚ transfer 590µL CaCl2 solution to the tube containing 50µL of the bacteria. 3. Tap the vial with the tip of your index finger to mix the solution. 4. Incubate
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AP Chemistry Period 1 Molecular Weight of a Condensable Vapor Lab Purpose: The purpose and objective of this lab was to find the molecular weight of a condensed vapor. Materials and Equipment: • Aluminum foil square (around 6cm on a side) • 125 mL flask • Barometer • 3 mL of unknown liquid • 200 mL graduated cylinder • 600 mL beaker • Pin • Balance (0.002g) • Bunsen Burner setup • Rubber band • Thermometer • Ceramic center wire gauze Procedure: 1. A 125 mL flask was obtained
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