components such as ligation buffer‚ DNA insert‚ pGEM®-T‚ and T4 DNA ligase is mixed by gentle pipetting. The 2X rapid ligation buffer is used in this experiment to saving the ligation time. This ligation buffer contains ATP. ATP is used to catalyze the formation of phosphodiester bond and the reaction of restriction enzyme buffer. The recommended time and temperature of incubation when using 2X rapid ligation buffer are one hour at room temperature (24°C) and overnight at 4°C. In this overnight incubation
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THE INCOMPLETE REPRAP BEGINNERS’ GUIDE v1 OKT 2011 HOW TO BUILD AND PRINT WITHOUT LOOSING YOUR MENTAL STABILITY COS_ (KONSTANTINOS TZIAMPAZIS) COS_ THE INCOMPLETE REPRAP BEGINNERS’GUIDE Prologue This is an attempt to gather in one document all the basic information about REPAP MENDEL PRUSA‚ that is available in the internet. The reason of doing it‚ is that it is sometimes more practical to have some basic information gathered in one place‚ than trying to get fast and accurate answers
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Ebanks v. New York City Transit Authority 70 N.Y.2d 621‚ 518 N.Y.S 2d 776‚ Web 1987 N.Y Lexis 17294 United States Court of Appeals of New York Facts: * Julius Ebanks’s left foot got caught in a 2-inch gap between the escalator step and the side wall of the escalator‚ which was owned and operated by the New York City Transit Authority. * He was thrown violently to the ground after reaching the top. His hip was fractured along with other serious injuries. * The standard gap of the
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When a population grows rapidly‚ it can put great stress on an area such as a community or a country. When sudden extreme population growth occurs‚ food will become a prized possession‚ more people will die due to disease and war‚ and the economy will be affected. As a population grows‚ all the additional people will need food immediately to survive. There will not be time to develop new sources of food over many years. Instead‚ more and more people will need food more and more and the food will
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“Rapid technological change has shaped the world we live in today…” The impact of new technology especially in the developed world is evident all around us in the way we communicate and understand the world. There has been significant progress in many spheres‚ such as health‚ engineering‚ education and many others with the technological progress. Sometimes it is far away from positive influence many areas of our everyday life depend on computers and mobile devices and it distanced us from what
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Name J. Martinelli Real World Technologies September 30‚ 2013 The Future of Commercial Shopping “3D printing is the process of creating an object using a machine that puts down material layer by layer in three dimensions until the desired object is formed” . The Apple Company recently released its newest line of phones‚ the iPhone 5s and the iPhone 5c. The 5c intrigued many because of its unfamiliar design; the phone is plastic! Imagine the initiative Apple has just bombarded upon the commercial
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3D Printing 3D printing[1] is a process of making a three-dimensional solid object of virtually any shape from a digital model. A materials printer usually performs 3D printing processes using digital technology. The first working 3D printer was created in 1984 by Chuck Hull of 3D Systems Corp.[3] Since the start of the 21st century there has been a large growth in the sales of these machines‚ and their price has dropped substantially. The 3D printing technology is used for both prototyping and distributed
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Direct to garment printer recent market conditions analysis The Economist magazine believes that 3D direct to garment printer printing technology represents new development trend of manufacturing industry‚ it will promote the implementation of the third industrial revolution. Currently two 3D printer worldwide manufacturing giants‚ respectively a Stratasys and 3DSystems‚2012-year‚ Stratasys shares doubled 3DSystems shares have increased almost twice‚ and the Nasdaq composite index has only risen
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The Future of Aviation Embry-Riddle Aeronautical University Abstract Aviation is a field full of unanswered questions and mystery. With the ability to fly almost anything or anyone‚ new concerns are being addressed. How can we make this mode of travel more efficient? How can we make it more practical? Everything comes at a cost. With todays craft polluting our air and fuel becoming more and more expensive‚ it is up to us‚ for the sake of mankind‚ to develop new technology to push this
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3D Printing 3D printing is becoming a new way for manufacturing. As oppose to regular manufacturing (subtractive)‚ where the machine cut out pieces of a raw material in order to shape the material to its final shape‚ in an additive manufacturing the machine adds layer of material and creates the final model. Additive process has many advantages over the subtractive one. Creating a part with 3D printing is much cheaper‚ makes less waste‚ faster and the result is even more accurate1. The most revolutionized
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