The total protein content was estimated as proposed by Lowry et al. A bovine serum albumin stock solution (1mg/ml) was prepared in sodium hydroxide (1N). Five different concentrations (0.2‚ 0.4‚ 0.6‚ 0.8‚ 1 ml) of the prepared solution were taken in different test tubes. In another set of test tubes‚ 0.1 and 0.2 ml of the extract were taken. In each test tube‚ the volume was made up to 1 ml‚ followed by addition of the prepared alkaline solution (5 ml) at room temperature. The solutions were left
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Reaction Rate Coursework Aim To see how the concentration of a reactant affects the rate of reaction‚ in this case sodium thiosulphate and acid. Scientific Background For a reaction to occur‚ the particles of the reactants must be colliding with each other with enough energy‚ the amount of energy needed for the reaction is called activation energy. Activation energy is altered by temperature‚ concentration‚ surface area of and the pressure on the reactants. Stirring the reactants also increases
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CHEMICAL REACTIONS OF COPPER AND PERCENT YIELD Objective To gain familiarity with basic laboratory procedures‚ some chemistry of a typical transition element‚ and the concept of percent yield. Apparatus and Chemicals |0.5 g piece of no. 16 or no. 18 copper wire |evaporating dish | |250 mL beaker (2) |weighing paper | |concentrated HNO3 (4 – 6 mL)
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Daphnia Heart Rate Background: In the world today‚ there are many chemicals that people use to fix or help with issues within their own bodies. Some of these chemicals may include things like caffeine‚ nicotine‚ and alcohol. These supplements can have substantial effects on other things within the body like the brain‚ liver‚ and especially the heart. For example‚ many chemicals like caffeine‚ nicotine‚ aspirin‚ and sleep aid all have effects on the body which can cause your heart rate to increase
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goldfish’s metabolic process at two different temperatures a chamber and Logger-Lite‚ computer software‚ were used during this lab. However‚ only data from lab section 1F was analyzed for this report. The goal was to determine if the goldfish’s metabolic rates‚ as measured by oxygen consumption‚ will increase when the water temperature is moderately warm. Alternatively‚ the metabolic rates of goldfish‚ as measured by oxygen consumption‚ will not differ between warm and ambient temperatures. Materials and
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which more clearly displays the relationship‚ view Figure 2. The rate at which the reaction occurred for the 100% concentration is 1.45 mg/dL per minute. For the 50%‚ the rate was 3.05 mg/dL per minute‚ and for the 25%‚ the rate was 2.76 mg/dL per minute. As seen‚ the rate fluctuated from the lowest rate at 100% and the greatest rate occurring at 50%. As mentioned earlier‚ the Collision Theory states the rate of a chemical reaction is proportional to the number of collisions that occur. One way to
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Abstract: The reaction used to determine the experimental rate law of is 2I-(aq) + H2O2(aq) I2(aq) + 2H2O2(l). The rate law determined experimentally is rate= k[I-]1.017[H2O2]0.927. Additionally by performing essentially the same experiments but with temperature changes one can determine how k is affected by temperature changes and the new activation energy. Also‚ from graphs the activation energy was determined to be 33.3 kJ/mol. INTRODUCTION: The rate of a chemical reaction often depends
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Lab Report Purpose: 1. To test Mariotte’s Law 2. To test Charles’ law 3. To test Gay-Lussac’s law 4. To test ideal gas law Theory: Mariotte’s law Charles’ law Gay-Lussac’s law Ideal gas law Apparatus: beaker‚ boiling water‚ thermometer‚ pressure-meter‚ oil‚ closed tube. Procedure: 1) Set up all the apparatus 2) Open the rubber cap. Move the closed tube several times to ensure that the oil is spread equally. 3) Pour
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Size‚ Temperature‚ Concentration‚ and a Catalyst on Reaction Rate Introduction: Chemical reactions can be affected by a number of different factors. Particle size‚ temperature‚ concentration of a solution‚ and catalysts play a big role in the rate of reaction‚ they determine how fast a reaction will occur. According to the collision theory‚ the rate of reaction depends on the frequency of effective collisions between particles. Every reaction is different in that they all require different conditions
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Iodine Isotopes are the atoms that have in their nucleus an atomic number corresponding to the chemical behavior of that element. Since isotopes have the same number of protons‚ they all have identical chemical behavior. But‚ however‚ since their neutron numbers are different the isotopes of the same element may have different radioactivity. An isotope that is radioactive is called a radioisotope like iodine‚ which is important in our health. In radioactivity‚ the nucleus of an unstable isotope
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