The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
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Lab Report #1 Photosynthesis Lab Report: The Effects of Varying Concentrations of CO2 on the rate of Photosynthesis in Spinach Leaves Introduction Photosynthesis is the process by which green plants and some other organisms use sunlight to synthesize foods from carbon dioxide and water. Photosynthesis in plants generally involves the green pigment chlorophyll and generates oxygen as a byproduct. The purpose of this experiment is to see how different concentrations of carbon
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PHOTOSYNTHESIS LAB Alyssa Wright VARIABLE: LIGHT INTENSITY AIM: to investigate the effect of light intensity (lux) on the amount of oxygen produced (number of bubbles) HYPOTHESIS: I predict that the rate of oxygen produced increases‚ as the light intensity increases‚ but only to a point. I predict this will happen because the plant will have enough carbon dioxide and water to keep up with the amount of light that there is. When there is less light intensity‚ photosynthesis will happen
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Through this experiment‚ Egeria densa was observed using a microscope. The task was to observe and identify the different types of cell‚ cytoplasmic streaming‚ and plasmolysis of Egeria densa. First‚ the microscope was examined and investigated to master the use of the equipment. A microscope slide grid which was on the slide glass was required to be seen clearly using 4x‚ 10x and 30x. During the latter part of the experiment‚ the Egeria densa was observed using the microscope to understand the
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AP Lab #5 Plant Pigments/Photosynthesis I. Identifying the Effects of Different Variables of Light and Carbon Dioxide on the Rate of Photosynthesis and Observing the Separation of Pigments Through Chromatography II. Introduction Plants have a variety of pigments‚ all of which absorb a different color of light. The three main pigments are chlorophyll a‚ chlorophyll b and carotenoids. Chlorophyll a is the primary plant pigment that absorbs red and blue light‚ which ultimately appears green to the human eye
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chemicals that control reactions in living cells. They are biochemical catalysts‚ speeding up reactions that would occur too slowly to be of any usefulness to an organism. Although in organisms‚ it is not necessary for the reactions to be at maximum rate at all times. Enzymes interact with other molecules to produce a stable system in which the products are made when they are needed in the exact amount. An enzyme lowers the activation energy of a reaction so its easier to proceed. Enzymes are specific
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Misael Acosta Photosynthesis Lab Report Ms. Rocha AP Biology 4th Period II. Intro/Background 1. In order for oxygen to be present photosynthesis must occur. Photosynthesis takes in light and CO2‚ and through a series of steps produces oxygen. Photosynthesis can be measured by either the disappearance of substrate or the accumulation of product. Examples include the production of O2 or the consumption of CO2. All the oxygen within the leaf disks will be removed before being placed
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An Increase in Light Intensity Increases the Rate of Respiration M. Chauntise‚ S. Shaika‚ R. Shantal INTRODUCTION The sun plays a vital role in sustaining an entire living world whether indirectly or directy using a transformation process called photosynthesis. This process assists in nourishing plants and keeping them alive. For example‚ trees grow very tall in the rainforest where there is an abundance of water in order to reach as much light as they can. For this reason‚ in order to survive the
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6‚ 2012 Photosynthesis Lab 1. Introduction: Light dependent reactions are the first phase of photosynthesis. It requires light to happen and happens in the thylakoid membrane in a chloroplast. The light energy is absorbed from the sun and converted into chemical energy. Which is then stored temporarily in ATP and NADPH. Hypothesis: I predict that the wavelength intensity will affect the rate of photosynthesis more than light intensity will. 2. Data: # ATP | Light Intensity (Lux) | Time
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starch molecules. The process by which this happens is called photosynthesis and results in the formation of oxygen and glucose. Plants take in carbon dioxide‚ water‚ and light then release oxygen back into the air. This process happens because of the movement of hydrogen to the hydrocarbons that are produced during photosynthesis. The discovery of photosynthesis is attributed to Dutch scientist Jan Ingenhousz who was the first to find that light is necessary to the process‚ it happens in the green parts
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