The purpose of this lab is to determine the rate of reaction under different circumstances. Different variables will be manipulated to discover their effects on a particular reaction. Changes in temperature‚ pH‚ and enzyme concentration are examples of factors that have the potential to affect the initial rate of an enzyme catalyzed reaction in a controlled experiment‚ whether it be speeding the reaction up or slowing it down. Part I of the experiment establishes a baseline that can be used to compare
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initial rate of reaction. I will measure the effect of the enzyme in 5 different concentrations against the controlled variable of the reactant. The enzyme which will be used is different concentrations of potato and the reactant used will be Hydrogen Peroxide. Hydrogen Peroxide which will be the buffer solution is a PH of 7.2. My hypothesis for the experiment is that as the concentration of the enzyme is increased the rate of reaction will be increased‚ producing oxygen at a faster rate. The results
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To investigate the results of the different amounts of sodium bicarbonate (2g‚4‚8g)‚when put in the baking process to produce cupcakes‚obtained by the change of height of a cupcake measured by a ruler‚when baked at 180 degrees celsius for 20 minutes when measured with a ruler. Research question: What will be the result of adding different amounts sodium bicarbonate (2g‚4g‚8g) in a standard (vanilla) cupcake ‚when they are baked at 180 degrees celsius over a period of 20 minutes using an oven thermometer
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Investigation of action of saliva and hydrochloric acid in two carbohydrate solutions. Objective: To determine the action of amylase and hydrochloric acid in two carbohydrates solutions Apparatus and equipments: 1. Boiling tube 2. Metal test tube racks 3. Beaker 4. Graduated plastic dropper 5. Water bath‚ ~37ᵒC 6. Water bath‚ ~95ᵒC Materials: 1. Carbohydrate solution A 2. Carbohydrate solution B 3. Benedict’s solution 4. 3M Hydrochloric acid 5. 3M Sodium hydroxide Procedures: 1. Two boiling
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Rates of Chemical Reaction II Pre-Lab Summary: First we will set up the experiment as shown in Figure 2‚ and then we will fill the trough with room temperature water and record the temperature and we will also add the room temperature water to the buret until it is about 10ml from the top. If there are no leaks in the apparatus‚ we will add 10ml of KI to 15ml of distilled water in a 125ml flask. We will swirl the flask so that it achieves room temperature‚ then we will add 5ml of H2O2 and quickly
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on the rate of reaction. By using self investigative and experimental skills‚ the experiment was done in order to determine how the rate of reaction will be altered‚ whether it will increase‚ decrease or remain constant when the different concentration of enzymes added. INTRODUCTION: Enzymes are produced naturally in plant‚ animal‚ and microbial cell. There are thousands of different enzyme can be found in any cell. Enzymes can be describe as molecules that are greatly increase the rate of chemical
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For known amounts of reactants‚ theoretical amounts of product can be calculated in a chemical process. These calculated products are determined using the limiting reagent‚ (the substance that runs out first which stops the reaction) and are called the theoretical yield. The theoretical yield is the amount of product that should be acquired during the experiment if all aspects go perfectly. However‚ due to experimental errors‚ very few labs do so. These results are called actual yield. The percent
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Factors that Affect the Rate of Reaction of Peroxidase Purpose: To determine the effect of various factors on the rate of reaction between an enzyme and its substrate‚ and also to determine the optimal ranges under which the enzyme activity is maximized. Also to determine whether saline and alcohol are inhibitors or activators Hypothesis: PH factor prediction: I predict that as the pH increases so the activity of the enzyme will increase until it reaches optimum pH range (pH 7) because the
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Sodium chloride‚ also known as salt‚ common salt‚ table salt‚ or halite‚ is an ionic compound with the formula NaCl. Sodium chloride is the salt most responsible for the salinity of the ocean and of the extracellular fluid of many multicellular organisms. As the major ingredient in edible salt‚ it is commonly used as a condiment and food preservative. [edit] Properties Thermal conductivity of pure NaCl as a function of temperature has a maximum of 2.03 W/(cm K) at 8 K and decreases to 0.069 at 314
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Atausch July 28‚ 2014 Bio 102 Lab Final Exam: Acids and Enzymatic Reaction Purpose: To examine how an acidic fluid will modify the outcome of an enzymatic reaction. Introduction: Enzymes are “specialized proteins made by living cells that serve as biological catalysts.” (eScience Labs‚ LLC‚ 2013) These enzymes “allow certain industrial processes to be carried out at normal temperatures and pressures‚ thereby reducing the amount of energy and expensive equipment needed.”(BBC‚ 2012) Enzymatic
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