VARYING EFFECTS OF ENZYME CONCENTRATION ON REACTION RATES OF MALATE DEHYDROGENASE CELL BIOLOGY 13 NOVEMBER 2007 Enzymes are biological catalysts. They are proteins that speed up reactions with low concentrations. These enzyme proteins are made up of linkages of amino acids. The links coil‚ and coil again forming a tertiary structure. This structure has a groove in it called an active site. The active site is
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Determining the activation energy in the reaction between crystal violet and sodium hydroxide. I. DESIGN ASPECT 1: definition of the problem‚ hypothesis and selection of variables Problem: How the Arrhenius equation can be used to determine the activation energy in the reaction between crystal violet and sodium hydroxide. Objective: The objective of the experiment is to determine the activation energy. Knowing the rate constant k of reactions between crystal violet and sodium hydroxide at
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Exercise No. 8 CARBOXYLIC ACIDS AND ACID DERIVATIVES I. OBJECTIVES: • To investigate the physical and chemical properties of Carboxylic acid and its derivatives • To understand the reactions of carboxylic compounds and derivatives. II. EXPERIMENTAL RESULTS Solubility of Carboxylic acids in 10% NaHCO¬3 Acetic acid - formation of bubbles Benzoic acid - formation of bubbles Test for Acetic acid NaOH + Acetic acid - blue litmus paper turned red NaOH + Acetic acid + FeCl3 - red colored
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The effect on rate of an enzyme catalysed reaction by different objectives which include effect of substrate‚ temperature‚ ph and effect of a competitive inhibitor phosphate ions. This is determined by the reaction of hydrolysis by p-nitrophenylphosphate (PNP) as a substrate by the enzyme phosphatase. Abstract The hydrolysis of p-nitrophenyl phosphate has been studied in human red blood cells. To see if hydrolysis was related to the functioning of the sodium pump. Acid phosphatase catalysis’s
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solution of an acidic and organic compound and purify its respective acidic product through the techniques of an acid base extraction‚ recrystallization‚ and use of Melt Temp (melting point range). The reaction of an unknown acid (3-chlorobenzoic acid) with diethyl and sodium hydroxide was observed‚ and the solution naturally separated into an aqueous and organic solution. The mixture of acid and organic solution was created and then separated through the Hirsch funnel into separate beakers of an aqueous
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Separation of a Carboxylic Acid from a Neutral Compound by Extraction Reference: Smith‚ Chapter 2 (Acids and Bases) Introduction Carboxylic acids and phenols are two families of organic compounds that contain carbon‚ hydrogen and oxygen‚ and also react with water to yield an excess of hydronium ions over hydroxide ions. Pure water has a pH of 7‚ which means it has a hydronium ion concentration‚ [H3O+] of 10-7 M (M = molarity‚ moles/Liter). The hydronium ions in pure water come from the self-ionization
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Chemical Reactions Introduction Pre-lab questions 1. Which reactants used in this experiment are flammable? Discuss the safety precautions that are necessary when working with flammable materials in the lab? 2. Summarize the following description of a chemical reaction in the form of a balanced chemical equation? 3. Common observations of a chemical reaction are described in the introduction section. For each observation‚ name a common or everyday occurrence that must involve a chemical reaction? Research
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CARBOXYLIC ACID Introduction: Organic compounds containing (–C(O)–OH) as a functional group are called carboxylic acids. The –C (O)-OH group which itself is made up of a carbonyl group (>C=O) and a hydroxyl group (-OH) is called a carboxyl group (carb from carbonyl and oxyl from hydroxyl group). Carboxylic acid may be an aliphatic or an aromatic depending upon whether –C–OH is attached to an alkyl group ( or a hydrogen atom) or an aryl group. Their general formulas are; ALIPHATIC CARBOXYLIC ACID: R–C
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Advantages: Sulfamic acid is the fastest de-scaler - It dissociates into hydroxonium ions more readily in aqueous solution than the others‚ therefore giving a greater concentration of atoms that are able to react with the calcium in lime scale. It is safe to use because it does not produce chlorine gas [5]‚ which can be toxic. Sulfamic acid also has a low volatility. Disadvantages: Sulfamic Acid can be an irritant to eyes or skin and is the most expensive of the de-scalers. Q2) How these de-scalers
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Vol. 1 ACETIC ACID 115 ACETIC ACID 1. Introduction Acetic acid [64-19-7]‚ CH3COOH‚ is a corrosive organic acid having a sharp odor‚ burning taste‚ and pernicious blistering properties. It is found in ocean water‚ oilfield brines‚ rain‚ and at trace concentrations in many plant and animal liquids. It is central to all biological energy pathways. Fermentation of fruit and vegetable juices yields 2–12% acetic acid solutions‚ usually called vinegar (qv). Any sugar-containing sap or juice can
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