and Substrate Concentration on Reaction Rate by Zachary A. Poché Biology 155 Laboratory October 15‚ 2014 Lab Partners: Cade White‚ Hannah Ragas‚ Russheka Aremillion ABSTRACT In order to increase the reaction rate‚ substrates attach to the active site of enzymes which decrease the activation energy required to convert substrates to products. We examined the effect of enzyme concentration and substrate concentration on the overall rate of the reaction. To determine the effect of enzyme
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catalysts that speed up chemical reaction but are not themselves consumed or changed by the reaction. The cell’s biological catalysts are proteins. Proteins are made up of one or more polypeptide chains that are folded to make an active site‚ an area in which a material to be acted on by the enzyme‚ called the substrate‚ will fit. The temperature‚pH‚ the concentration of enzyme‚ and the concentration of substrate all affect the activity of the enzyme and the rate of the reaction. The browning of potatoes
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part of random collisions‚ which produce chemical reactions. This is actually needed to sustain life. These collisions are influenced by many different physical factors‚ such as temperature or concentration. If more molecules are concentrated in a place‚ the more random collisions are more likely to happen. If temperature is higher the reaction is sped up‚ thus leading to more collisions. Enzymes are catalyst because they speed up reaction rates. They are shaped a specific way‚ so when they collide
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Paperose Lab- Introduction to Enzymes Ms. Kim/Honors Biology Purpose To Determine the rate of an enzyme reaction using a “modeled” enzyme and sugar‚ paperase and paperose. Introduction In this lab‚ your hands are the enzyme‚ paperase. This enzyme split the sugar‚ paperose‚ into subunit A and subunit B. You will split this molecule by ripping the paper model down the middle. Materials Paperose models‚ scissors‚ plastic bag‚ container (large plastic cup)‚ stopwatch‚ Procedure 1. Each
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My lab group studied the effect of PH on reaction rate/ enzyme activity measured by foam height. PH is the measure of the concentration of hydrogen ions in a solution. The higher the hydrogen ion concentration‚ the lower the pH. Every enzyme has an optimal PH‚ meaning they have a very small window in which they are most active. Our enzyme (potato smoothie) had an optimal PH of 7.0-7.5. We know this because we measured the enzyme’s reaction rate by measuring foam height. The largest foam height we
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in temperature in the reaction of catechol and catecholase will not change the absorbance of reaction over time. What was your alternative hypothesis? be specific As the environment and temperature is changed from 0 °c to 20°c to 95°c‚ the absorbance of catechol and catecholase reaction will increase over time. The rate of reaction increases as the temperature increases. Results: What were the main trends or patterns in your data (Results)? In the Icebath reaction at 0°c has a mean concentration
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Mapua Institute of Technology Organic Chemistry Laboratory 2 Final Report Factors Affecting the Relative Rates of Electrophilic Aromatic Substitution Reaction Justiniano‚ Priscilla Raiza N. School of Chemical Engineering and Chemistry‚ Mapua Institute of Technology‚ Intramuros‚ Manila‚ Philippines Experiment No.1‚ Submitted on August 6‚ 2011 at N402. Abstract EXPERIMENT NUMBER ONE IS ALL ABOUT THE ELECTROPHILIC SUBSTITUTION OF AROMATIC COMPOUNDS. AROMATIC COMPOUNDS ARE THOSE ORGANIC
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catalyst in chemical reactions. Catalyst are used to increase the rate of a chemical reaction. In this study‚ we performed two different experiments that investigated the effect of varying substrate concentration‚ and the effect of temperature on the rate of Enzyme-Catalase reaction. In experiment one (i.e. the effect of varying substrate concentration on the rate of enzyme-catalase reaction) we tested the hypothesis HA as substrate concentration increases‚ so will reaction rate until all active sites
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concentration speed up the reaction rate of amylase in starch? In this experiment diluted solutions of amylase were created and then tested using a starch solution‚ I2KI for reaction times. The answer to the question was proved to be that more concentration of amylase speeds up the reaction time. Introduction The enzyme‚ amylase is found in the saliva of most animals and in humans. Amylase hydrolyzes starch‚ a plant’s reservation of carbohydrates. Amylase causes a chemical reaction in the polysaccharide
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Rates of reaction Sodium Thiosulphate and Hydrochloric Acid An experiment was carried out to investigate the effect of temperature on the rate of reaction between sodium thiosulphate and hydrochloric acid. The equation for this reaction is shown below. Method (1) A measuring cylinder was used to measure 50cm3 of 0.08M sodium thiosulphate solution into a test tube. A bung was placed in the test tube. (2) A pipette was used to measure 5cm3 of 1.0M hydrochloric acid and this was placed into a second
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