The effect of increasing substrate concentration on rate of an enzyme reaction. Enzymes are biological catalysts that lower a reactions activation energy making possible many of the reactions needed for life to exist. Enzymes have a high specificity which have been explained by many theories such as Fischer’s lock and key. Currently the most widely accepted theory is the induced fit hypothesis proposed by Koshland in 1958. This hypothesis solves some of the problems with the Lock and key theory
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(Tinnesand‚ n.d.). A strong acid ionises completely while a weak acid only ionises partially. Furthermore‚ the strength of the acid will also have an effect on the rate of the reaction. A strong acid will have a faster reaction then
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Introduction “The Enzyme Reaction” An enzyme is a protein that acts as a catalyst‚ which brings out a biochemical reaction. A Catalase enzyme‚ the enzyme tested in this experiment‚ is found in almost all living organisms that are exposed daily to oxygen (such as fruits‚ vegetables and animals). Background Information The Catalase enzyme in this experiment is known for being less affective the warmer the temperature is. According to “Science fair projects” an enzyme becomes unstable
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between the Diffusion Rate of a Substance and its Molecular Weight ABSTRACT To test the effect of molecular weight on the rate of diffusion‚ various experiments were performed. One of which is the glass tube test wherein cotton balls of the same size were moistened in two different substances (NH4OH and HCl). These cotton balls were plugged at each side of a glass tube. After some time‚ formation of a white ring occurred. The white ring‚ in fact‚ is a product of the reaction between the molecules
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Abstract This experiment is to study the effect of temperature on the rate of reaction between potassium permanganate with oxalic acid. We used 2cm3 of 0.02M potassium permanganate and 4cm3 of 1M sulphuric acid into a test tube. In another test tube‚ we placed 2cm3 of oxalic acid. We placed the test tubes in a water bath at 40‚ 45‚ 50‚ 55 and 60oC respectively. When the solutions have attained these temperatures pour the oxalic acid into the acidified permanganate solution and recorded the time
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In class we decided that the rate of a reaction increases when there are more collisions or when the activation energy is reduced. We then determined that there were four ways to increase the reaction rate: increase temperature‚ add a catalyst‚ increase surface area‚ and increase the concentration of reactants. The objective of this lab was to experimentally show how temperature and surface area impact the rate of reaction‚ so only two of these methods were tested. The evidence indicates that increasing
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of an object is determined using a process called titration. According to Petrucci et al.‚ titration is a reaction carried out by the carefully controlled addition of one solution to another. It has several types like acid-base titrations and redox titrations. This experiment focuses on the latter type‚ the redox titration. A redox titration is based on an oxidation-reduction reaction between analyte and titrant (Harris‚2009). It involves the transfer of electrons between the two solutions. This
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Reaction Rates of Barley Alpha Amylase with Starch at Specific pH ’s and Temperatures Page 1 Abstract Enzyme ’s are used as catalysts in certain reactions. They help lower the activation energy needed for the reaction to go to completion. At optimum temperature and pH the amount of collisions of substrate and enzyme is at its highest‚ any deviation from the optimum temperature and pH will result in the denaturization of the enzyme. The purpose of this experiment is to find the optimum
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factors affecting the rate of a chemical reaction. Introduction: In my coursework I will be mainly concentrating on the reaction between dilute hydrochloric acid with marble chips (calcium carbonate). Calcium carbonate (marble chips) + hydrochloric acid ==> calcium chloride + water + carbon dioxide CaCO3(s) + 2HCl (aq) ==> CaCl2 (aq) + H2O (l) + CO2 (g) Aim: The aim of the experiment is to investigate how the concentration of hydrochloric acid affects the rate of reaction with marble chips‚
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thiosulfate solution on the rate of the reaction when reacting with dilute hydrochloric acid. It is hypothesized that as the concentration of the sodium thiosulfate solution increases‚ the rate of the reaction will also increase proportionately. Variables The independent variable is concentration. This will be varied by increments of 10mL (10mL to 50mL) and distilled water will be added accordingly to maintain a constant volume of 50mL. The dependent variable is the rate of the reaction. This will be indirectly
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