Formation of an Alkene by Alcohol Dehydration Lab Report Balanced Chemical Equation for the Main Reaction Mechanism The acid-catalyzed dehydration of secondary and tertiary alcohols involves non-isolable carbocation intermediaries. In the first step of the of the reaction mechanism below‚ a phosphoric acid catalyst adds a proton to the oxygen atom of the alcohol to form an oxonium ion. The OH is converted to a better leaving group as the positive charge on the oxygen weakens the carbon-oxygen
Premium Alcohol Oxygen Water
Chemistry lab on Alcohols Cristina Tolosa Jhony Arias Data Collection: Table One: absorbance recorded with the different substances Try one Try two Substance Absorbance Substance Absorbance 4ml of water‚ 0 ml of ethanol 0.0000 4ml of water‚ 0 ml of ethanol 0.000 3ml of water‚ 1 ml of ethanol 0.125 3ml of water‚ 1 ml of ethanol 0.058 2 ml of water‚ 2ml of ethanol 0.147 2ml of water‚ 2ml of ethanol 0.087 1ml of water‚ 3ml of ethanol 0.153 1ml of water‚ 3 ml of ethanol 0.099 0 ml of
Premium Ethanol Concentration Alcohol
the limiting reactant of a chemical reaction that involves iron filings and a copper sulfate solution. Second‚ to determine the mole ratios between iron and copper and to show that a single replacement reaction occurs when iron and copper sulfate react with each other. And lastly‚ to determine/calculate the percent yield to better analyze the chemical reaction between iron and copper sulfate to produce copper and iron sulfate. The method used to perform this lab will be to combine a metal with a solution
Premium Chemistry Chemical reaction Hydrogen
Reactions in Aqueous Solutions: Metathesis Reactions and Net Ionic Equations Introduction: Metathesis or double decomposition reactions are a reaction in which two compounds react to form two new compounds‚ with no changes in oxidation number. The ions of two compounds exchange partners. AX + BY AY + BX This reaction can occur between two inorganic salts when one product is insoluble in water‚ driving the reaction forward. A typical example is as followed and is considered a molecular equation
Premium Sodium chloride Chlorine Chemistry
Abstract The experiment is to identify the guilty suspect that is present at the crime scene by comparing with the DNA samples. Polymerase Chain Reaction(PCR) is used to amplify the small amount of Deoxyribonucleic Acid (DNA) for forensic or genetic studies‚ which require necessary product and placed in the thermal cycle. Gel electrophoresis is being run in order to analyze and compare the DNA samples at the crime scene with the guilty suspects. Gel electrophoresis is used to separate DNA using
Premium DNA DNA Polymerase chain reaction
purpose of this lab was to find the theoretical and experimental percentage yields of the double displacement reaction between the solutions Lead (II) Nitrate (PbNO3) and Potassium Iodide (KI). It is important to obtain amounts of Lead (II) Nitrate and Potassium Iodide as close to 1.44g as possible. This reaction creates Lead (II) Iodide and Potassium Nitrate. The precipitate during this reaction is Lead (II) Iodide. The balanced equation is Pb(NO3)2 + 2KI= PbI2 + 2KNO3. In this lab the Lead (II) Iodide
Premium Chemistry Chemical reaction Solubility
March 18‚ 2012 Title: Types of Chemical Reactions Data: Reaction Observations Evidence #1 It turned a reddish brown and the liquid went clear. The color change proved it was a chemical change. #2 The reaction turned yellow and it separated and created a fog. The solid formed precipitation and had a color change during the reaction proving it to be a chemical change. #3 There was green smoke and the crystals turned black. The green liquid turned dark green and continued to sizzle with
Premium Chemical reaction Chemistry Solid
Physical and Chemical Reactions Lab #4 Alesa Cannon Objective: To investigate the physical and chemical properties of a pure chemical substance. Procedure: 1. Half-fill one well of a 24-well plate with 6 M HCl and half-fill a second well of the 24-well plate with 6 M NaOH. Suck up these chemicals into their labeled pipets for later use. 2. Perform the following steps on each of the substances to be tested. Complete all tests of one substance and record your observations before proceeding
Premium Chemistry Chemical reaction Hydrogen
test tube D and E. The metal spreader was then sterilized again for any future uses. These plates for C‚ D‚ E‚ and the streaking plate were left upside down‚ taped and labeled with the section and lab ID number and then incubated for 2 days at 30 degrees Celsius and refrigerated until the following lab
Premium Laboratory glassware Agar plate Petri dish
The procedure was taken from “From Drosophila cDNA in E. coli plasmid to homologous human proteins” lab manual (4). - Colony Picking: Two E. coli colonies were grown on agar plates and treated with ampicillin. They contained the plasmid with genes for ampicillin resistance and Drosophila cDNA sequence. - Plasmid Isolation: We used the QuickLyse Miniprep Plasmid DNA purification systems to isolate the plasmid DNA. Indeed‚ the bacterial cells were removed from the liquid broth and were resuspended
Premium DNA Gene Molecular biology