Experiment 1: Isolation of Casein from Milk * pH of milk – 6.6 * milk = 87.1% water‚ 4.9 % CHO 3.9 %‚ fats‚ 0.7% minerals Experiment 2: Protein Hydrolysis and Characterization | Reagents | Principle | Test for | Positive Result | Negative Result | Biuret | CuSO4‚ NaOH | Complexation of Cu+2 with amide N atoms | Polypeptide bonds | Violet/purple solution | Blue color solution | Sakaguchi | 10% NaOH‚ 0.02% α-naphtol solution‚ 2% NaOBr | arginine condenses with α-naphtol and NaOH
Premium Acetic acid Iodine Ester
Table 1. Observations from week 2 for the detection of ammonia using the Nessler’s reagent and from week 1 for the pH using bromothymol blue indicator with the inoculation of P. vulgaris‚ P. fluorescens‚ and B. Cereus in peptone broth. Tubes were incubated at room temperature for 7 days and 14 days. Soil Microorganism Nessler’s Reagent (color reaction pH (bromothymol blue) Our results pH (bromothymol blue) Class results P. vulgaris Deep yellow ++ 8.0 8.0‚ 7.5‚ 6-7‚ 11.5 P. fluorescens
Premium Ammonia PH PH indicator
ev milk‚ 50% egg solution‚ 1% sucrose‚ 4 test tubes‚ 1 test tube rate‚ safety glasses‚ pipets and the reagent solution. For test 2 for starches the following materials were used: Di water‚ 1% starch solution‚ onion juice‚ sucrose solution‚ iodine reagent‚ 4 test tubes‚ test tube rack and pipets. In test 1 and test 2 each solution was placed in a separate test tube with the corresponding reagent added to each as well. In test one for proteins each substance that showed a purple color it indicated
Premium Glucose Purple Carbohydrate
The purpose of this experiment was to synthesize triphenylmethanol from a Grignard reagent. The Grignard reaction technique was used in this synthesis but due to the fact that it is such a strong nucleophile and base‚ it was important to prevent water from interfering with the Grignard reaction. Purity of the product was determined by measuring the melting point. Reagent Table: Structure Name Molecular formula Molar mass Density Melting point Boiling Point Diethyl ether C4H10O
Premium Magnesium Oxygen Diethyl ether
141–143. Grasshoff‚ K. Ehrhardt‚ M‚ and K. Kremling (1983). Methods of Seawater Analysis. Grasshoff‚ Ehrhardt and Kremling‚ eds. Verlag Chemie GmbH. 419 pp. Murray J.N.‚ Riley‚ J.P. and Wilson‚ T.R.S. (1968). The solubility of oxygen in Winkler reagents used for the determination of dissolved oxygen. Deep-Sea Res.‚ 15‚ 237–238. Strickland‚ J.D.H.‚ and Parsons‚ T.R. (1968). Determination of dissolved oxygen. in A Practical Handbook of Seawater Analysis. Fisheries Research Board of Canada‚Bulletin
Free Titration Iodine
analyze the moisture content of a given sample using Karl Fischer volumetric titration technique. Theory: Karl Fischer volumetric titration is used to detect analytically the moisture content present in samples. The iodine present in the Karl fischer reagent reacts stoichoimetrically with the amount of water present in the solution. For example water reacts with iodine in 1:1 mole ratio in the reaction: I2 + SO2 + H2O + 3Base + CH3OH → 2Base HI + Base HSO4CH3 From the amount of excess iodine present
Premium Titration
Abstract: The purpose of this lab was to synthesize triphenylmethanol from benzophenone and bromobenzene by the formation of a Grignard compound with the reagents bromobenzene and magnesium metal. The bromobenzene was first transformed into the Grignard compound and was then reacted with the benzophenone to make the final product. The mixture was then mixed with sulfuric acid and the organic layer was extracted via a separatory funnel. The mixture was then recrystallized from methanol and was allowed
Premium Magnesium Diethyl ether Solvent
use of Grignard reagents. The percent yield of the product was 10% on a relatively humid day. The melting point was calculate to be 127.2oC with a literature value of 162oC. An IR spectrum of the product was taken and used for positive identification of the product. The mechanism of the formation of triphenylmethanol was explored. Byproducts were also considered and their mechanisms duly noted. Introduction: This experiment explores the use of organometallic reagents in addition reactions
Premium Oxygen Chemical reaction Alcohol
Grignard reaction‚ a Grignard reagent (R–MgX) adds to the carbonyl group in an aldehyde or ketone to form an alcohol (Figure 1). The reaction of a Grignard reagent with formaldehyde can be to synthesize a primary alcohol‚ with any other aldehyde can be used to synthesize a secondary alcohol‚ while the reaction with ketone is useful in the synthesis of a tertiary alcohol. Figure 1. General reaction mechanism of a Grignard Reaction The preparation of the Grignard reagent involves the adding of an alkyl
Premium Acetic acid Acid Chemistry
violet color when dyed. The experiment was first heated under reflux to produce the necessary Grignard reagent as a grey liquid. It was then reacted with diethyl carbonate and hydrochloric acid to produce crystal violet. The resulting chemical was very absorbent to polypropylene and bleached cotton fabrics. Introduction: In this experiment a Grignard reaction is performed with a Grignard reagent acting as the nucleophile1. Grignard reactions are very useful for the substitution of compounds containing
Premium Chemistry Oxygen Diethyl ether