Today‚ we have to learn basic laboratory techniques‚ such as handling the reagents‚ using Gilson pipettes etc‚ study and try to identify chemical tests for food compounds. Materials and methods Reagents Monosaccharaides (glucose‚ fructose‚ maltose)‚ sucrose (disaccharide) Starch (polysaccharide)‚ egg albumin (protein) DCPIP‚ Vitamin C Olive oil + Sudan III Ethanol (highly flammable) Iodine solution (irritant) Benedicts reagents KOH + CuSO4 HCl (irritant) Honey solution Apple juice solution Milk solution
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Composition of Cells Testing for Proteins: The independent variable is the biuret reagent. The dependent variable is the solutions being used as follows; distilled water‚ albumin solution‚ and starch solution. If the protein is present‚ then the biuret will change to a violet color. Authors: Eliza Koundry and Jody Cote Eliza was in charge of adding equal amounts of biuret reagent to the distilled water‚ albumin solution‚ and the starch solution. Jody was in charge of recording
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oxidation. Sodium hypochlorite was used instead of the less “green” Jones reagent. Borneol was mixed with glacial acetic acid and oxidized with sodium hypochlorite to make crude camphor. The crude camphor was purified by sublimation. The final product was characterized by obtaining a melting point and inferred spectroscopy. The experiment was carried out to see if bleach could oxidize borneol and if it is greener than using a Jones reagent. Sure enough camphor was made with a 4.03 g of unsublimed camphor
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SUBJECT: CLINICAL CHEMISTRY TITLE: TOTAL BILIRUBIN & DIRECT BILIRUBIN LABORATORY: 19 & 20 INTENDED USE: 1. For the quantitative determination of total bilirubin in serum 2. For the quantitative determination of direct bilirubin in serum INTRODUCTION: One part of bile is a yellow (or brownish) substance called bilirubin. Bilirubin starts out as a product of the breakdown of red blood cells. When red blood cells break down‚ the heme molecule is converted into bilirubin
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Keywords acid value vegetable oils indirect titration A new method of acid value determination in vegetable oils has been developed. The method is based on (a) simple‚ rapid and complete extraction of acids from an oil test portion into reagent (0.05 mol dm–3 triethanolamine (B) in the mixture of 50 % H2O + 50 % 2-PrOH) and (b) indirect titration of acids in BH+ form against aqueous alkali in the presence of a phenolphthalein indicator. Suitable metrological parameters of acid value determination
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kidneys‚ or other regions of their bodies. The dialysate contains waste matter that flows from the blood our of the dialysis tubing. Question/aim of lab: To test the solutions inside and outside the dialysis tubing with Benedict’s solution‚ Iodine reagent and silver nitrate solution to find out what has remained in the dialysis tubing‚ and which solutes have diffused through the semi permeable membrane and into the dialysate. Hypothesis: The sodium chloride will definitely pass through the semi
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drops-no change in color The positive tubes were the tubes with the egg white‚ pepsin‚ and sugar. Protein was found in the first two substances. Biuret reagent can be used in a real life situation to test the detection of protein in food. The predicted results were the same as the results. Exercise 2: Testing for Sugar To add Benedict’s reagent to four substances‚ glucose‚ starch‚ onion juice‚ and distilled water‚ to test the detection of sugar in each. Test Sample Predicted Results Benedict’s
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4-Dihydroxyphenylalanine CONDITIONS: METHOD: REAGENTS: A. 50 mM Potassium Phosphate Buffer‚ pH 6.5 at 25° C (Prepare 50 ml in deionized water using Potassium Phosphate‚ Monobasic‚ Anhydrous‚ Sigma Prod. No. P5379. Adjust to pH 6.5 at 25° C with 1 M KOH.) 1 mM L-Tyrosine Solution (Prepare 100 ml in deionized water using L-Tyrosine‚ Free Base‚ Sigma Prod. No. T-3754.) Tyrosinase Enzyme Solution (Immediately before use‚ prepare a solution containing 500 - 1‚000 units/ml of Tyrosinase in cold Reagent A.) T = 25° C‚ pH = 6
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The purpose of this lab experiment was to take the reagents of magnesium and Bromobenzene to create the Grignard reagent of phenylmagnesium before its converted to the final product of triphenylmethanol. Then the product was purified via methods such as recrystallization and filtration‚ before it was finally characterized via a series of methods such as TLC‚ melting point‚ IR‚ H NMR‚ and C NMR. Before the lab even began‚ the first thing done to ensure a safe successful reaction was to oven dry all
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NPTEL – Chemistry – Reagents and Organic reactions Module II Lecture 14 Reduction Reactions 2.1.1 Lithium Aluminum Hydride (LAH) 2.1.1.1 Introduction Lithium aluminum hydride (LAH) is a strong reducing agent with chemical formula LiAlH4. It can reduce a variety of functional groups such as aldehydes‚ esters‚ acids‚ ketones‚ nitriles‚ epoxides and azides. It vigorously reacts with water and all the reactions are performed in polar aprotic solvents. 2.1.1.2 Preparation It was first
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