2 test tubes were compared. For aldehydes Tollen’s reagent was used‚ 1 mL was placed into both test tube along with one have 2 drops of the unknown #12 and another with 2 drops of the aldehyde. For Ketones a reagent called phenylhydrazine (C6H5NHNH2) was used‚ 1 mL of it was added to both test tubes with one test tube having 2 drops of the unknown and the other the 2 drops of ketone. For the alcohols 3 test tubes were used‚ 2 mL of Lucas reagent was added to each test tube with one having 5 drops
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ml-1) (ml) 0.0 0.2 0.4 0.6 0.8 1.0 Biuret reagent (ml) 4.0 4.0 4.0 4.0 4.0 4.0 Total Volume (ml) 5.0 5.0 5.0 5.0 5.0 5.0 Final protein concentration (mg ml-1) 0 2 4 6 8 10 Absorbance 0.000 0.092 0.163 0.272 0.363 0.474 Table 2: Experimental protocol for determining the protein concentrations of silverbeet grown in the sun and shade Sun Shade Column for modified procedure(s) Procedure Protein concentration (ml) 1 1 0.4 0.4 Biuret reagent (ml) 4 4 4 4 Absorbance 1.104 0.908 0.465 0
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oats‚ and other staple foods. www.LabPaq.co !4 ©Hands-On Labs‚ !! !! ! ! ! !! !! ! !! Experimen ChemiCal Basis of Exercise 5: Testing Breakfast Cereal Observations: ! Material tested Data Table 5: Cereal testing results biuret reagent Benedict’s reagent
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conclusions about the experiments they have performed. This lab utilized both quantitative and qualitative data by using the Paper Chromatography method for determining the metal cations present in an unknown sample. This was performed by using reagents that reacted with the metal cations deposited on the paper. The retention factor of each known cation was calculated in order to determine which metal cations were present in two unknown samples. Objective: Effectively use Paper Chromatography
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barium chloride as a reagent to precipitate sulfate from the dissolved sample. The mass of sulfate in the sample can be calculated by simple stoichiometry from the mass of the weighing form of the sulfate precipitate. In order to ensure maximum filterability and minimum contamination‚ the sulfate is precipitated under conditions that maximize the particle size. These conditions include: (a) avoiding excessively high concentrations of precipitating reagent; (b) slow addition of reagent to a hot solution
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What do you understand by the term “Lancefield groups”? History In the early 1930’s Rebecca Lancefield recognized the importance of serological tests for the identification of organisms. The system is based on specific antibody agglutination reactions with cell wall carbohydrate antigens (C polysaccharides) extracted from the streptococci. Lancefield also showed that further sub division of group A into specific serological types was possible using type-specific M (protein) antigens. The groups
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Limiting Reagent and Percent Yield Aim To determine the limiting reagent between the reaction of lead (II) nitrate and potassium iodide. To determine the percent yield of lead (II) iodide. Date Started: 13/4/12. Finished: 19/4/12. Data collection and processing Measurements: * Amount of distilled water: 75.0ml ± 0.5ml. * Mass of watch glass: 31.65g ± 0.01g. * Mass of watch glass + potassium iodide: 32.45g ± 0.01g. * Mass of potassium iodide: 0.8g ± 0.02g. * Mass of watch
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to a colored or fluorescent product antigen Components of VIDAS System • reagent strip • solid phase receptacles (SPRs) • fluorescence optical system • automatic pipetting system Solid phase receptacle (SPR) Reagent Strip • 1 sample well • 8 reagent wells – washing solutions – enzyme conjugated antibody – enzyme substrate Single reagent strip • 1 cuvette • covered with a foil seal Dual reagent strip (with reference) See notes‚ p.2 VIDAS Steps and Principle 1. Sample
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are four main macromolecules; proteins‚ nucleic acids‚ lipids‚ and carbohydrates. The presence of some of these‚ such as sugar‚ fat‚ starch‚ and protein‚ can be determined using tests such as Benedict’s solution‚ Lugol’s iodine solution‚ Biuret’s reagent‚ and Sudan III. Control tests were performed to determine what a positive result would appear like. Then the tests were performed using substances containing sprite‚ fries‚ hamburgers‚ and chicken nuggets. Results matching the control results would
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and get him the proper help. Materials and Method/Results: Materials required to perform tests are the following: A microscope‚ inoculating loop or needle‚ Bunsen burner‚ glass slides‚ lens paper‚ bibulous paper‚ regular tap water‚ along with reagents Crystal violet‚ Gram’s Iodine‚ 95% ethyl alcohol‚ and safranin. The first test I conducted was the gram stain. In preparation for the actual gram staining process I had to make a smear. To do this‚ I acquired a clean glass slide. I then placed
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