is absent‚ the solution is a red-brown colour and when the protein binds‚ the pKa of the dye moves causing the dye to become blue. The anionic blue form of the dye‚ which binds to the protein‚ has a maximum absorbance of 590 nm whereas the assay reagent solution of red and green forms has a maximum absorbance of 470 nm and 650 nm. Due to the assay being sensitive with a range of 20 to 200 μg protein‚ it can be determined by the amount of dye present in the blue ionic form. This can usually be accomplished
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Oxidation of an Alcohol: Oxidizing Methoxybenzyl Alcohol to Methoxybenzaldehyde Using Phase-Transfer Catalysis PURPOSE OF THE EXPERIMENT Oxidize methoxybenzyl alcohol to methoxybenzaldehyde‚ using sodium hypochlorite as the oxidizing agent and tetrabutylammonium hydrogen sulfate as the phase-transfer catalyst. Monitor the progress of the reaction by thin-layer chromatography. BACKGROUND REQUIRED You should be familiar with extraction‚ evaporation‚ and thin-layer chromatography techniques
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Testing for reducing sugars‚ non-reducing sugars‚ starch‚ lipids and proteins in unknown substances: Our aim: was to find out which substances from our five samples have reducing sugars present to determine what they may be for example; they could be monosacchrides or disaccharides. This was carried out by using Benedict’s test. To find out which substances from our five samples were non-reducing sugars‚ since some disaccharides are reducing sugars and this would be carried out by using Benedict’s
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indicating that O2 (and water) were being released. Although enteric bacteria use oxygen as a terminal electron acceptor‚ tests for oxidase activity indicated that they possess terminal oxidases other than cytochrome c oxidase. That is‚ the oxidase reagent did not change from pink to purple when it came in contact with any of the organisms‚ indicating a negative result. Instead of oxygen‚ some facultative anaerobes employ nitrate as a terminal electron receptor; the nitrate broth was used to test for
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tissues and then returns carbon dioxide to the lungs. HbG can be used to diagnose or monitor a number of conditions. Anemia is one of those conditions. List required reagents: Drabkins Solution is the reagent that is required for HgB. Drabkins reagent composed of potassium cyanide and potassium ferricyanide. The reagent is used to lyse red cells and convert hemoglobin to cyanmethemoglobin in hemoglobinometry. 2. Equipment needed when doing the HbG procedure is a spectrophotometer which is
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Correct answer: e. tubes 3 and 4 An optical density greater than zero indicates that Your answer : a. BAPNA digestion has occurred. Correct answer: e. BAPNA digestion has occurred and pepsin is active. Experiment Data: Tube No. Reagent 1 Reagent 2 Reagent 3 Boiled Time Temp. 1 2 3 4 5 6 Pepsin Pepsin Pepsin DeIonized Water Pepsin Pepsin BAPNA BAPNA DeIonized Water BAPNA BAPNA BAPNA pH 2.0 Buffer pH 2.0 Buffer pH 2.0 Buffer pH 2.0 Buffer pH 7.0 Buffer
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Preparation of Dibenzalacetone by the Aldol condensation David o Neill Date of experiment: 14/12/2011 Apparatus Steam bath‚ ice bath‚ Buchner funnel‚ beaker‚ conical flask‚ filter paper‚ TLC apparatus‚ Melting point apparatus Materials / chemicals Benzaldehyde‚ acetone‚ ethanolic sodium hydroxide‚ ethanol Introduction The synthesis of dibenzalacetone is formed from an Aldol condensation reaction. An Aldol condensation reaction is a very effective way of forming a carbon – carbon bond reaction‚ in
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0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to determine absorbance with a spectrophotometer. Two additional samples were made; one was blank and the other was for the specific homogenate sample. Then 3 microliters of bradford assay reagent‚ which indicates the amount of protein present by color‚ was added to all samples. The spectrophotometer was zeroed at 595 nm. A standard curve was made with the different absorbencies and concentrations. After the linear equation was formed‚ the
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reacts with reductive substances such as organic impurities in water‚ ashes in air and so on‚ it easily decomposes when exposed to light. When it is prepared‚ its solution must be boiled or be dissolved with cold distilled water and then kept in brown reagent bottle in dark. The primary standard
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BIOLOGICALLY IMPORTANT MOLECULES Abstract Compounds containing the element carbon‚ called organic compounds‚ are the most important substances that make up living organisms. There are thousands and thousands of different organic compounds. To identify the major types of organic compounds in living organisms ‚ several biochemical test were used . Introduction Organic molecules are those primarily made up of carbon‚ hydrogen and oxygen. The common organic compounds of living organisms are
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