Introduction At any particular wavelength the absorption of light when visible light passes through a solution depends on two factors: * The length of the light path * The concentration of the colored path The connection of these two variables is known as the Beer-Lambert Law: Absorbance (A) = ε c l Where ε is the absorption coefficient C is the concentration of the compound And l is the length of light usually 1cm When I is constant‚ this proves a linear relationship between
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until the concentration is equilibrium. There are usually more solvents in the water inside the plant which means there is a high concentration. Because of this‚ the water flows into the root hair cells from the soil. Watering plants with a saline solution (salty water) changes the osmotic potential of the soil. This results to the water surrounding the root more saline than the cell sap within the plant. This causes reverse-osmosis where the nutrients are actually drawn out of the plant and into the
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reduced. You are to plan an experiment to investigate how the boiling point of an aqueous solution of potassium chloride depends on the concentration of the solution. (a) (i) By considering how the vapour pressure changes as the concentration of the aqueous potassium chloride increases‚ predict and explain how the boiling point of the solution will be affected by the concentration of the solution. Predict how the boiling point will change
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in different solutions while trying to reach equilibrium? Background Information Osmosis is the procedure where water or different types of liquids move through a semipermeable membrane. This type of passage is considered as simple diffusion where no energy is required. This means that the liquid will have to leave the cell or enter it so that equilibrium can be reached. Equilibrium is the state reached when the amount of concentration is equal between the cell and the solution it is placed
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moles of a solute by the volume of the solution in liters. Multiple series of solutions with different concentrations can be used by diluting the concentration. The dilution technique is: Number Moles Concentrated Solution = Number Moles Dilute Solution. An instrument called a spectrophotometer detects the amount of light that passes through the sample and the percent transmittance can be recorded from the meter. In the lab‚ multiple homogeneous solutions are made. There was not a way to determine
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Osmosis Osmosis is a special example of diffusion. It is the diffusion of water through a partially permeable membrane from a more dilute solution to a more concentrated solution – down the water potential gradient) Note: diffusion and osmosis are both passive‚ i.e. energy from ATP is not used. A partially permeable membrane is a barrier that permits the passage of some substances but not others; it allows the passage of the solvent molecules but not some of the larger solute molecules. Cell
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incorporated Beer’s Law and is focused on determining the stress that various alcohols have on biological membranes. Using five solutions of differing alcohol concentration for each of the three alcohols; methanol‚ ethanol‚ and 1-propanol and a small slice of beet‚ the stirred solution was placed into a plastic cuvette and then into a spectrophotometer and the absorbance of alcohol solutions were determined in order to conclude which alcohol and concentration of alcohol had the greatest effect on biological
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Directions Using the formula for dilutions‚ write the recipe to make 100 mL of a new solution of each solute based on having as much of the initial solution as you need. V1 M1 V2M2 SoluteFormula of SoluteInitial Concentration (M)Final Concentration (M)Recipe For 100 mL of New SolutionSodium hydroxideNaOH1.0 M0.27 M(1.0M)(VNaOH) (0.27 M)(100 mL) VNaOH 27 mL Dilute 27 mL of 1.0 M NaOH to 100 mL.Potassium permanganateKMnO41.0 M0.34 MDilute 34 mL of 1.0 M
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Beer Lambert Law experimentally. The various solutions used for this experiment are tap water mixed with food colouring‚ Introduction: The Beer Lambert Law shows the relation between absorbance of light of an object‚ the molar absorptivity‚ the concentration of the substance‚ and the distance the light travels. The Beer Lambert Law states that there is a linear relationship between the concentration of a solution and the absorbance of said solution. If there is a linear relation‚ the resulting
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resulting solution was filtered through multiple layers of cheese cloth to filter out the liquid by eliminating any large pieces in the solution. The solution created was catechol. Five different solutions were prepared as blanks with each test tube containing 6.0mL of a different pH (pH 4‚ pH6‚ pH7‚ pH8‚ pH10) of phosphate buffer‚ 1.0mL of the enzyme and 1.0mL of water. Five more solutions were prepared in the same fashion except without the 1.0mL of water. These five experimental solutions were capped
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