Abstract Over a two week period of time in the laboratory‚ we experimented and tested the reaction rate of a peroxidase enzyme and the factors that affected it‚ both positively and negatively. The purpose of these experiments was to probe and manipulate the activity of the enzyme peroxidase by varying temperature‚ pH‚ the amount of enzyme compared to the substrate and the effect of hydroxylamine. Peroxidase activity is expressed when the potato extract is subjected to stresses such as low temperature
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respiration (Evert RF‚ Eichhorn SE & Perry JB 2013). This experiment focuses on the enzyme catalase. Catalase breaks down hydrogen peroxide into water and oxygen. Hydrogen peroxide is a waste product of cell metabolism that can be toxic to the cell (Evert RF‚ Eichhorn SE & Perry JB 2013). The
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Title Antimicrobial properties in different type of plants. Introduction A substance that kills or prevents the growth of microorganisms for example bacteria‚ fungi or protozoans is called an antimicrobial. This substance has 2 major roles which are to either kill microbes (microbiocidal) or prevent the growth of microbes (microbiostatic). Disinfectants are antimicrobial substances used on non-living objects outside the body. This substance included antibiotics‚ antifungals‚ antiprotozoals and
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Tracheostomy Tube A tracheostomy tube‚ or trach tube‚ allows a person to breath without using his or her nose or mouth. Some trach tubes have a cuff on the end that keeps air from reaching the mouth and nose. A cuffless tracheostomy tube does not have a cuff. Since there is no cuff‚ a person with this kind of trach tube can cough and speak normally. Two people are needed to change a cuffless trach tube. SUPPLIES NEEDED Clean gloves. A new trach tube and an emergency trach tube. One tube should
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Salt from green beans Name: Ashni Couprie and Dennise Susanto Class: 5c Introduction From previous experiments we ’ve learned that salt is extracted from green beans. To be more specific: salt is extracted from when the beans are cooked in water in which salt has been dissolved. This experiment is based upon the osmoses theory. According to this theory the cell walls of the green beans are what we call a semi permeable membrane. This membrane only lets water through and not the substance
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PROCEDURE Part I Setting Up Experiment 2D: Separation of a mixture by Paper Chromatography Objectives: 1. To assemble and operate a paper chromatography apparatus 2. To study and the significance of Rf values 3. To test various food colorings and to calculate their Rf values 4. To compare measured Rf values with standard Rf values 5. To separate mixtures of food coloring into their components 6. To identify the components of mixtures by means of their Rf values Materials / Apparatus: See “Health
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heart rate if the Daphnia increases. Equipment: * Culture of water flea – Daphnia * Caffeine of 5 different concentrations * Microscope * Counter * Cavity Slide * Pipettes * Stop clock * Distilled water * Test tubes * Petri dish Method: 1. Remove one Daphnia and place it in the cavity slide 2. Remove the pond water and replace it with distilled water 3. Leave it for 5 minutes to acclimatize‚ then observe the heart rate under the microscope for
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were mixed and shaked in a 4-inch test tube. The mixture was centrifuged‚ and the supernate was decantated into a test tube. Separate drops of the supernate was tested for presence of Fe2+‚ Ag+ and Fe 3+ in three different test tubes: Ions Test Reagent Observation Fe2+ 0.10M K3Fe(CN)6 Prussian blue precipitate Fe 3+ 0.10 M KSCN Blood red solution Ag+ 1.00 M HCl White precipitate Copper (II) – Ammonia System 1.00 M NH3 was added dropwise to a test tube filled with 1ml of 0.1 M CuSO4. Contents
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Should we use animals for experiments? (against) The question is‚what it means to use animals for experiments? It means‚ the investigators and doctors whose are producing medicines they have to test is first‚somehow‚to see how is the effect of the medicine.This “somehow” means the animals.Moreover I haven’t said anything about testing new products like cosmetic products‚make-ups‚sprays. In my opinion‚the animals are different‚they body and organism are different.For this reason the scientists
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Experiment 9 Thin Layer Chromatography Experiment: 9 Title: Thin Layer Chromatography Introduction: Chemical analysis is conducted on specific analytes (specific substance of interest in a mixture)‚ however it is often found that these analytes must be separated for the chemical analysis to conduct their analysis. Chromatography is a set up of laboratory a technique that is used to separate a chemical mixture. This technique is very useful as it allows us to follow the course of
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