Extraction and Analysis of Plasmid DNA from E. coli Cells Introduction A plasmid is an extra-chromosomal element‚ often a circular DNA. Since a plasmid is by definition an extra-chromosomal element‚ it cannot make use of any origin of DNA replication in a chromosome (BP site). Meaning that DNA synthesis within a plasmid depends on having an origin of DNA synthesis of its own. Plasmids are often found in bacterial cells‚ in which they are used as transfer agents for transmitting various antibiotic
Premium Molecular biology DNA Plasmid
Samoranos‚ Jesha Joy R. 2011-12518 ENG 105 – C The Map to Playwriting To travel to any place‚ for example‚ from UPLB to Enchanted Kingdom‚ one may take the jeepney or drive his private vehicle. Of course‚ this trip must have been planned and prepared for beforehand. Either through commute or carpool‚ land transportation generally use automobiles powered by fuel that pass through roads that lead to a specific destination. A driver or a commuter must know where he must go and how to get
Premium Play Theatre Writing
Petition on Gun Restrictions in the United States We‚ the undersigned‚ believe that these gun restriction laws which have been passed have no benefit towards our country; so that we demand that these laws should be more enforced from our government‚ updating the gun restriction laws to be more effective‚ and that no more gun restrictions laws are to be passed; as guns have been used to save the lives of people up to one hundred times more than to injure someone’s life‚ background check funding has
Premium Firearm Gun Gun politics in the United States
Akamai Case Digest 1. Why did Akamai charge content providers and subsidize ISPs? Akamai Technology operated the content delivery network. Its first service‚ FreeFlow‚ facilitated the delivery of bandwidth-intensive page elements. A content provider could tag the object that it wanted to serve over the Akamai network. After tagging‚ websites could be over 10 times faster and be protected against sudden traffic bursts. FreeFlow could reduce the content providers’ payments to ISPs for bandwidth
Premium Mixed economy Public transport
References 1. Potter and Perry 2. Nursing diagnosis cards by Taylor and Ralph 3. Mosby’s Nursing skills (website) 4. Clinical nursing skills (Smith) Diagnosis 2 (Maslow’s hierarchy of needs : safety) Impaired physical mobility related to Open Reduction‚ Internal Fixation (ORIF). As evidence by Pt. having intrameduillary interlocking rods in right and left bilateral femurs (3). Physician order pt. to remain in bed and catheter placed in patient’s bladder‚ which leads to and
Premium Pain Patient Wound healing
Restriction Enzymes Restriction enzymes (also known as restriction endonucleases) are a group of bacterial enzymes which cut double-stranded DNA (dsDNA) into smaller fragments at specific points. They are a defence mechanism used by bacteria to cleave the DNA of invading viruses‚ thereby restricting their expression. The exploitation of restriction enzymes ability to cut large pieces of DNA into smaller fragments (called restriction fragments) and the highly specific way in which they do this
Premium DNA
recreation for many reasons. They may or may not impact our activities‚ but they some how influence us to take pleasure in our everyday work. I believe social capital is an influential factor in my personal leisure time. When constructing my seven day map I realized that I have been privileged with activities and facilities. I am a very athletic and active person‚ so taking advantage of local gyms is a big part of my leisure time. Social capital is the most dominant factor because the economic benefits
Free Leisure Recreation Personal life
obtain one that doesn’t have any restrictions? Well‚ the answer to this is relatively simple. States have implemented laws to aid teen drivers on their new adventure of driving a vehicle. There are two steps to these restrictions that teen drivers have to face. The first restriction is the "driver licensing programs" which requires teens to obtain a set number of years after getting their learner’s permit to get a regular driver’s license. The second restriction is the‚ "limiting distracted driving
Premium Driving Driver's license Adolescence
Week 1 | Day | English | Mathematics | Science | Social Studies | Integration Possibilities | 6 | Literary ELACC2RL1-10‚ Informational ELACC2RI1-10‚ Narrative ELACC2W3 Comprehension of Fiction‚ Phonics‚ Writing | GA CCSS 2.2OAAdd and Subtract within Twenty | S2CS2 a-c: Computation & Estimation skills; S2CS5 a‚b: Communicate scientific ideas | GA SS2H2 a‚bDescribe the Georgia Creek and Cherokee cultures | ART- During History class‚ students can work in groups to create murals that showcase
Premium Fiction Writing Mathematics
Communication and Critical Thinking Research Essay 1 Topic: Privacy Restrictions of Facebook – Unquestionable or Unachievable Date of Submission: 25th October 2010 It is alarming how fast technology is improving. Nowadays‚ it seems like having a Facebook account is a daily necessity or even a trend. According to a research done by TIMES magazine‚ more than one in four people who browse the Internet not only have a Facebook account but have returned to the site in the past thirty days. It
Premium Facebook