Experiment title: Extraction of Bacteria Plasmid DNA and Analysis of extracted DNA Samples Objectives: 1) To study and understand the steps for extract bacteria plasmid DNA. 2) To measure the concentration and purity of extracted DNA by using spectrometric method and agarose gel electrophoresis method. 3) Determine the size of extracted DNA by using agarose gel electrophoresis method. Materials and Methods: (Refer to UDEE2124 lab manual from page
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Title. Restriction Enzyme Mapping of pBR322 Using Agarose Gel Electrophoresis. II. Authors. Author: Partner: Section: Thursday‚ 1:10 pm Date of Experiment: October 25‚ 2012 III. Introduction. Restriction enzymes (or restriction endonucleases)‚ originally isolated from Haemophilus influenzae in 1970‚ are enzymes within a cell that cleave foreign DNA within a specific and predictable nucleotide sequence (known as a restriction site) regardless of the source of such DNA. Such restriction sites
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Discovery Restriction enzymes were discovered 40 years ago during investigations into the phenomenon of host-specific restriction and modification of bacterial viruses. Restriction enzymes protect bacteria from infections by viruses‚ and it is generally accepted that this is their role in nature. They function as microbial immune systems. When a strain of E. coli lacking a restriction enzyme is infected with a virus‚ most virus particles can initiate a successful infection. When the same strain
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Freedom or Restriction Imagine living in the 1860’s as an African-American. You are beginning to get your rights to vote and your gaining your freedom. In1860‚ the census revealed that the national population was 31‚000‚000. Out of the 31 million‚ only 4.5 million of them were black (14percent) Only 221‚000 free blacks lived in the north which is 20 percent of the black population. By the end of the 1800’s‚ most Northern Blacks were free. But did Northern blacks really free or did they have more
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Over Immigration Restriction The term immigration refers to moving from your native country and coming to a foreign land for the purpose of a permanent residence and searching for greener pastures. There are several arguments by scholars about immigration restrictions to the United States. They had several similarities supporting immigration restrictions. Prescott Hall‚ Robert Ward‚ Frank Wright‚ Frank Fetter and John Mitchell all argued supporting the immigration restrictions. Immigrants
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CASE DIGESTS: 1. ALCANTARA vs. ALCANTARA FACTS: On Dec. 8‚ 1982‚ petitioner Restituto Alcantara and respondent Rosita Alcantara‚ without securing the required marriage license‚ went to Manila City Hall for the purpose of marriage. They met a “fixer” and arranged everything for them. A Rev. Aquilino Navarro‚ a Minister of Gospel of the CDCC Chapel solemnized their wedding. The petitioner and respondent went through another marriage ceremony on March 16‚ 1983. The marriage was likewise celebrated
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E. Coli Transformation with Plasmid (pGal)‚ pGal Isolation‚ and Analysis of Plasmid DNA Felicia Osadi Bio 22 April 20‚ 2012 Transformation = group 10 Plasmid = group 7 RFLP = group 1 RESULTS Table I. Plasmid Transformation of E. Coli. Plate # | Agar plate | Type | Result | 1 | X-gal | Control | Extensive lawn growth | 2 | Ampr / X-gal | Control | Clear no bacterial growth | 3 | Ampr / X-gal | Transformation | 1 blue colony | Transformation efficiency = 1 transformants
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Insertion and amplification of EGFP protein into pET41a(+) Plasmid Introduction The overall purpose of the experiments conducted is to test the creation of recombinant plasmid using recombinant DNA technology. The research of recombinant DNA began with the use of E.Coli (Escherichia coli)‚ a common bacteria found in the intestines of warm-blooded or- ganisms (5). Scientists worked together and generated a way‚ from cloning and using recombi- nant research‚ to achieve recombinant DNA
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Michael Anthony L. Leachon Obligation and Contracts Case Digests PNB VS CA 315 SCRA 309 FACTS: * October 23‚ 1990 Lily S. Pujol opened an account with the petitioner PNB(Mandaluyong branch.) She applied for a “combo account” under her business name “Pujol Trading.” This account is a combination of a savings and current account wherein if there are insufficient funds in the current account of the owner then the funds of the savings fund will be used. PNB thereafter‚ issued a passbook with
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I believe that World Wide Web restrictions should not be allowed. I believe that they are not helpful to the people that use the World Wide Web. I feel that the restrictions on the World Wide Web at school are too strict. At school most sites you try to view are prohibited and they are totally harmless sites. I feel that at school the only restrictions that should be put on the World Wide Web are restrictions to pornographic sites. Even these sites should not be blocked because some harmless
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