Transformation Of Escherichia Coli With pGLO Plasmid April 24‚ 2013 ABSTRACT: This experiment focuses on genetic engineering and transformation of bacteria. The characteristics of bacteria are altered from an external source to allow them to express a new trait‚ in this case antibiotic resistance. In is experiment foreign DNA is inserted into Escherichia coli in order to alter its phenotype. The goal of the experiment is to transform E. coli with pGLO plasmid‚ which carries a gene for ampicillin
Premium Bacteria Escherichia coli DNA
This experiment is to determine the unknown DNA plasmid using restriction enzymes and conducting electrophoresis finally comparing the resulting fragments with the known restriction map. In this lab‚ it succeeds in showing the fragments. In this report we will discuss the‚ results‚ limitations and possible errors. Introduction In biology restriction enzymes are used in several ways to modify and manipulate DNA molecules. One common use is to compare pieces of DNA from one that is unknown‚ with fragments
Premium DNA Molecular biology
2014. Title: Ligation of Lambda DNA pre-digested with EcoRI and HinDIII. Restriction of Lambda DNA with restriction enzymes. Aim: The objectives of this experiment are: Become more familiar with using micropipettes. Use restriction enzymes to cut DNA at specific sites. Use Ligase to rejoin some of the cut/separated DNA fragments. Learn to separate DNA using electrophoresis. Introduction: Restriction enzymes are proteins which cut dsDNA at specific regions depending on the enzyme
Premium DNA Molecular biology
ASUNZA‚ Charles Darvin S. 4LM RESTATE THE FOLLOWING: 1. ARTICLE 279 No less than the Constitution recognizes and guarantees the labor’s right to security of tenure. Under the Labor Code of the Philippines‚ as amended‚ specifically‚ Article 279 of the said Code‚ the security of tenure has been construed to mean as that “the employer shall not terminate the services of an employee except for a just cause or when authorized” by the Code. The two facets of this legal provision are: (a) the
Premium Employment
New Product Success/Failure Paper Apple Maps Vs. Google Maps Intro: Global Positioning Systems (GPS) is a space based satellite navigation system that provides location and time information all over the globe where there is no obstruction to the line of sight to the GPS satellites. The GPS project was developed in 1973 to overcome the limitations of previous navigation systems. It was originally designed for military use by the U.S. Department of Defense. Advances in technology and the
Premium Global Positioning System Google
GUANZON‚ Aldrin James L. PA 122 REGALA‚ Marc Daniel d. J. Hilda G. Diokno Lademora‚ Carl C. vs. CSC‚ Feb‚ 05‚ 2007 Facts: On February 3‚ 2006 petitioner‚ the duly elected Mayor of San Francisco‚ Agusan Del Sur‚ filed a Motion for Reconsideration of the invalidation of the approval of the temporary appointment of Daligdig with the CSC-CARAGA‚ where the case contains the following relevant portions: a) On July 5‚ 2005 Mayor Lademora appointed‚ Gil A. Daligdig as Municipal
Premium Municipality Mexico City Local government
Salazar‚ Kristine Kim B. 4-DLM 1. Arturo Tolentino vs Secretary of Finance FACTS: Tolentino et al is questioning the constitutionality of RA 7716 otherwise known as the Expanded Value Added Tax (EVAT) Law. Tolentino averred that this revenue bill did not exclusively originate from the House of Representatives as required by Section 24‚ Article 6 of the Constitution. Even though RA 7716 originated as HB 11197 and that it passed the 3 readings in the HoR‚ the same did not complete the 3 readings
Premium Tax Value added tax Indirect tax
Facts: Respondent is a Master Sergeant of the Phil. Air Force and the petitioners‚ Redentor Complate‚ employee of co-petitioner Elpidio Abaiad. Albayda was on his way to the office to report for duty‚ riding a bicycle ‚ the taxi driven by Completo bumped and sideswiped him‚ causing serious physical injuries. The Office of the City Prosecutor issued a resolution as Albayda said in his filling complaint‚ recommending the filing of an information for reckless imprudence resulting physical injuries
Premium Tort Law Jury
Common Carrier De Guzman vs Court of Appeals G.R. No. L-47822‚ December 22‚ 1988 Ponente : Feliciano‚ J.: Facts: Ernesto Cendana was engaged in buying up used bottles and scrap metal in Pangasinan and upon gathering sufficient quantities of such scrap material‚ respondent would bring such material to Manila for resale. On the return trip to Pangasinan‚ respondent would load his vehicles with cargo which various merchants wanted delivered to differing establishments in Pangasinan. For that
Premium Cargo Legal terms Transport
Thornton vs. Thornton‚ G.R. No. 154598 August 16‚ 2004‚ J. Corona Facts: Petitioner‚ an American‚ and respondent‚ a Filipino‚ were married in Manila. A year later‚ respondent gave birth to a baby girl. However‚ after three years‚ respondent grew restless and bored as a plain housewife. She wanted to return to her old job as a "guest relations officer" in a nightclub‚ with the freedom to go out with her friends. Whenever petitioner was out of the country‚ respondent was also often out with her friends
Premium Supreme Court of the United States Habeas corpus Court