Question: Evaluate the relative importance of the following as factors prompting Americans to rebel in 1776: *parliamentary taxation *British military measures *restrictions of civil liberties *the legacy of colonial religious and political ideas. Introduction: a. Time Frame/organization of the paper: As a result of the Seven Years’ War‚ Britain was buried in debt caused by instigating war to safeguard the colonies. Manipulating the colonies into taxation in order to pay debt resulted in resentment
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technology ever replace books as source of information? No. For me‚ the best source of information is books and technology is just an option only. The advantages of developing technology are: First‚ it is convenient to surf the Internet without the restrictions of time‚ distance and environment. People can search what they want at
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Restriction Endonuclease Digestion of DNA from E. coli cells and Analysis by Agarose Gel Electrophoresis Introduction The main goals of this experiment are testing an alternative procedure called “boiling lysis”‚ evaluating the quality of the purified plasmid for restriction digests‚ and identifying the mislabeled plasmid. The plasmid DNA from a carrier E. coli strain was purified by the boiling lysis. In the boiling lysis method‚ the bacterial cells were given momentary heat treatment
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Too much emphasis is placed on testing these days. The need to prepare for tests and examinations is a restriction on teachers and also exerts unnecessary pressure on young learners. To what extent do you agree or disagree? | | |In many schools and academic institutions throughout the world today there has been a shift from formal examinations and tests
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PLASMID DNA ISOLATION‚ RESTRICTION ENZUME DISGESTION AND AGAROSE GEL ELECTRIPHORESIS Abstract: The gel is covered with an ion- containing buffer‚ such as (TAE)‚ that controls the pH of the system and conducts electricity. overall DNA concentration was lower than expected. Using agarose gel electrophoresis is to separate and visualize the DNA fragment‚ which is produced by restriction enzymes . Introduction: The purpose of this experiment is to measure the size of the fragments of DNA and separate
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Instead of seeing greater labour mobility‚ globalization today has resulted in many countries imposing in-flow travel restrictions on certain nationalities seeking better work opportunities. Does this mean that globalization has failed? Discuss. First of all before we can discuss whether globalization has failed or not we need to understand what globalization is. In simple terms globalization is the integration of cultures‚ and this process stems from the interaction of people from different cultures
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replicate there 3) Insertion is carried out by cutting the vector and the DNA to be inserted with the same restriction endonuclease to ensure that both have the same "sticky ends" Vector for cloning the bacteria come from two major types: (1) Plasmids‚ and (2) Phages Recombinant DNA Technology A) It was developed in 1970 B) Procedure: (1) Obtain DNA from an organism (2)ADD Restriction enzymes (3) A reproducible set of fragments (4)Inset into replicating DNA molecules (5) This gives Recombinant
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vector p7012 is shown. The restriction enzymes listed cut only where indicated; they do not cut anywhere else in the vector or insert. a) A schematic of gene W is below. You want to clone all of gene W DNA into the p7012 vector. Give three different strategies that you could use to clone gene W into p7012‚ and obtain colonies that contain a recombinant plasmid. * Strategy 1 uses the restriction enzyme(s) ______Kpnl________ to cut the vector and restriction enzyme(s) ____Kpnl_________
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* Antibiotic production * Recombinant DNA Restriction Enzymes * Bacterial enzymes: cut up foreign DNA * Specific: only but at recognition sequences * Palindromic: cut at the same base sequence on each strand‚ but in the opposite direction * The exposed bases provide “sticky ends” * H-bond to compliment bases of segments cut with same restriction enzyme Recombinant DNA 1. Isolate DNA from 2 sources * Bacterial plasmid w/2 genes * ampR(resistance to ampicillin)
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using a restriction enzyme digest; after which the cloned genes were inserted into the bacteria E. coli through the transformation process consisting of a restriction enzyme digest and ligation. Colonies of the bacteria were grown; plasmids from each colony were purified‚ and then
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