Clinical Chemistry Manlangit‚ Joyce Ann U. EXPERIMENT Title: Standard Curve Preparation Objectives: 1. Properly set up a standard curve 2. Using the prepared curve‚ determine the concentrations for control and patient specimens. Materials and Reagents: 2ml 1M Sulfuric Acid 2ml of 0.1M Potassium Dichromate Distilled Water Pipettes Volumetric Flask Test Tubes Procedure: 1. Prepare 1 ml glucose solution and place in test tubes 2. Add 2ml of 1M Sulfuric Acid solution 3. Add
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Johnson Spectrophotometer; the finding of protein concentration of an unknown sample of BSA‚ and by using the standard curve. Abstract A spectrophotometer’s purpose is to use colors of the light spectrum to determine the concentration of light absorbing molecules in a solution. (p.59) In this particular lab‚ our mission was to determine the protein concentration and the standard curve of the unknown sample of BSA. This‚ by preparing five dilutions of the unknown solution of BSA together with
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bell curve is literally‚ the symmetrical curve created on a graph when using a frequency distribution method for a set of data‚ splitting the mean symmetrically. There is a big difference between standard deviation and the bell curve! Standard deviation shows the difference in variation from the average; the bell curve‚ also normal distribution or Gaussian distribution‚ shows the standard deviation and is created by the normal or equal distribution of the mean among either half. The bell curve is an
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Date: 09/21/2012 Result The purpose of the experiment is to measure the amount of DNA extracted from a strawberry. In the experiment‚ we measured the absorbance of different concentrations of Methylene Blue. The data collected by our group is presented on Figure 1 and the average absorbance measured by whole class is stated on Figure 2. Table 3 shows the measurement on the amount of strawberry DNA extracted. In Figure 1 and Figure 2‚ the data will be presented Standard Curve Graph. The relationship
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Sugar Metabolism Title: The effectiveness of glucose and fructose metabolism in individuals of resting and exercising observed through blood glucose concentration. Aim: To study the mechanisms of the body involved in absorbing and metabolizing sugar in the form of glucose and fructose and examine the effect of exercise on the rate of removal of glucose from the bloodstream. Introduction: The blood glucose concentration in the blood is interceded by the human body via negative feedback mechanism
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Construction of a Standard Curve for the Determination of Protein Concentration 09/13/12 PCB 3023-007 Results Figure1: Standard curve. This graph was constructed from the fixed amounts of Bovine Serum Albumin (BSA). Varying amounts of BSA at a concentration of .5µ/µl were mixed with the water and 1mL of coomassie dye. The equation y=0.0259x +0.0511 represents the best fit line for a data set taken from µg of protein equals two to ten µg. Optical density (OD) was surveyed using a Mach
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Glucose homeostasis The fed state Presentation copyright © 2007 David A Bender The central nervous system is very largely reliant on glucose as its metabolic fuel; it cannot oxidise fatty acids (but can metabolise ketone bodies in prolonged starvation) glycogen Red blood cells are absolutely reliant on glucose; they have no mitochondria and form ATP only by anaerobic glycolysis triacyglycerol protein glucose triacylglycerol in VLDL triacylglycerol in chylomicrons amino
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well did the results compare with your prediction? The molecules were too large to pass through. This is what I predicted on my Hypothesis. 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? The glucose diffused through the 200 MCWO membrane while the albumin didnt. 4. Put the following in order from smallest to largest molecular weight: glucose‚ sodium chloride
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Glucose Oxidase and Its Various Uses Aaron Truong Since glucose oxidase has an end product called hydrogen peroxide‚ which is a harmful substance to bacteria‚ it can be used to fight bacteria‚ or sterilize objects (can have various uses such as in hand sanitizers‚ toothpaste‚ soap‚ etc)‚ not just biosensors. Another key part in the reaction would be C6H12O6‚ or glucose. Glucose oxidase can be applied to diabetics as mentioned earlier‚ as biosensors work by "keeping track of the
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complex carbohydrates include vegetables‚ whole grains‚ peas‚ pasta‚ potatoes and beans. Carbohydrates are the main source of glucose‚ which is a major fuel for all of the body’s cells and the only source of energy for the brain and red cells. Except for fiber‚ which cannot be digested‚ both simple and complex carbohydrates are converted into glucose. The glucose is then either used directly to provide energy for the body‚ or stored in the liver for future use. When a person consumes more
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