pH and Chromatography Lab Report Kevin Rivera Biology Mr. Langley 2C 10/7/13 Introduction In this lab‚ of pH and Chromatography‚ in the pH aspect of the lab we are trying to figure out the pH level of certain chemicals by writing down of known solutions to find the type of unkown solution using pH standards. In the Chromatography‚ we are trying to separate the chemicals using water and a piece of paper. Methodology Materials: Safety Goggles pH indicator & pH indicator key
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Organic Chemistry 12A Professor Alston October 28‚ 2014 Isolation of Chlorophyll and Carotenoid Pigments from Spinach Purpose The purpose of this experiment was to isolate ß-carotene‚ chlorophyll-A‚ and chlorophyll-B from spinach using column chromatography. Spinach was dehydrated using ethanol‚ and the pigments were extracted with dichloromethane. The spinach extracts were dried using CaCl2. Then‚ the solid pigments were run through a column using a non-polar solvent‚ hexane. The polar absorbent
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tested for by a possible employer include Cocaine (crack)‚ Amphetamines (crystal)‚ Opiates (codeine‚ morphine‚ heroin)‚ PCP (phencyclidine)‚ and Marijuana. Gas chromatography/mass spectrometry is used to test hair and urine samples of possible drug abusers or job applicants‚ and it is the best method for the testing of drug use. Gas chromatography and mass spectrometry are two different methods for identifying chemical substances‚ and the two instruments have be coupled together to perform a highly complementary
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Alkenes from Alcohols: Analysis of a Mixture by Gas Chromatography Aim: To analyze a mixture of alkenes by gas chromatography. Introduction: In this lab‚ we specifically used elimination reaction; however we only used the E1 reaction. In the presence of strong acids‚ alcohols protonate to form a good leaving group‚ namely water. Upon loss of a proton to a good leaving group‚ an introduction of unsaturation (a double bond) can be preformed. According to Wikipedia‚ an E2 reaction is typically
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A stationary phase is on the plate; in this lab silica was used. A small amount of the sample being analyzed is spotted onto the bottom of the plate. A small amount of solvent with the appropriate polarity is put in container. This is called the eluent. The plate is placed upright in the container
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Chromatography Lab Questions Pre-Lab Questions 1. A pencil is used rather than a pen to mark the “starting line” in step two because a pen or marker while be used to place a small dot of ink in the center of the X that is made with a pencil. 2. Capillary action is filling of pores in a solid with a liquid. Capillary action is caused by spotting or absorption and is allowed to seep or flow through. 3. The type of pen that will probably give better separation in this paper chromatography
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Unit 2 Home Labs Goals: • Read Safety • Perform Changes: Physical or Chemical Lab • Perform Special Physical Properties of Matter Lab • Perform Paper Chromatography Lab Turn-in: 1) Changes: Physical or Chemical Lab - Completed Procedure / Observations‚ questions‚ and a written description of most important concept that you learned in the discussion section. 2) Special Physical Properties of Matter Lab – Completed Procedure / Observations‚ questions‚ and
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customer & BSNL. Method : Private RF modems in local lead from nearest Mobile BTS by customer Procedure : 1. Customer will apply higher BB‚ ILL or P2P leased line with own RF arrangement in end link / local lead. 2. BSNL will provide output on E1 or Ethernet from OFC CPE of nearest BTS and allow it on private RF. 3. One end of RF wil be BSNL mobile BTS and other end will be customer premise. Both will be installed / maintained by customer through his vendor. 4. RF IDU will be placed in BTS room
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Back Laboratory Program SAFETY IN THE CHEMISTRY LABORATORY 786 Pre-Lab Extraction and Filtration 790 1-1 Mixture Separation 792 1-2 Water Purification 794 3-1 Conservation of Mass 798 4-1 Flame Tests 801 Pre-Lab Gravimetric Analysis 828 13-1 Separation of Pen Inks by Paper Chromatography 830 13-2 Colorimetry and Molarity 834 14-1 Testing Water 838 804 7-1 Separation of Salts by Fractional Crystallization 806 7-2 Naming Ionic
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Experiment 5 Analysis of Analgesic Tablet by High Performance Liquid Chromatography Abstract An unknown sample‚ 529‚ was tested using high performance liquid chromatography to detect the concentrations of acetaminophen‚ aspirin‚ and caffeine respectively. There was found to be 4.03±0.144mg/100mL of acetaminophen‚ 11.5±0.185mg/100mL of aspirin‚ and 4.89±0.185mg/100mL of caffeine. Based on accepted values‚ the maximum daily amounts of each compound are 4000mg of acetaminophen
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