In this TLC lab‚ we explored the relative polarities of Acetylsalicylic Acid‚ Ibuprofen‚ Acetaminophen‚ and Caffeine based on their functional groups. The most overall polar analgesic is considered to be the analgesic that contained the most polar function groups. The amine and amide functional groups are the most polar. In this experiment‚ we found that both these functional groups are found in Caffeine. As a result‚ Caffeine was the most polar out of Acetylsalicylic Acid‚ Ibuprofen‚ Acetaminophen
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reacting salicylic acid with FeCl3‚ finding its melting points‚ and by doing Thin-layer chromatography. When you reacted salicylic acid with FeCl3‚ changes in color will occur. Pure Aspirin has a melting point around 135°C and a pure salicylic acid has 158 °C. TLC is used to separate compounds based on affinity for stationary phase or mobile phase. In TLC‚ the solvent went up at different rates. The retention factor (Rf) of a component can then be measured by dividing the distance that was traveled by
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Krotec; Honors Bio Period 4 Photosynthesis Lab Report I. Introduction The photosynthesis lab is designed to quantify photosynthesis‚ enabling a number of variables to be tested for their effects on photosynthetic rate. The production of oxygen‚ is used as an indirect measure of photosynthetic activity.The lab allows the examination of the effects of different wavelengths of light on photosynthetic activity. The other aspect of the lab includes Paper chromatography‚ which is a useful technique for separating and identifying pigments and
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blue and red wavelengths have more absorbance‚ the green doesn’t have a high absorbance. This lab is useful to help demonstrate the existence of various leaf pigments by using the process of paper chromatography.
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sodium hypochlorite as the oxidizing agent and tetrabutylammonium hydrogen sulfate as the phase-transfer catalyst. Monitor the progress of the reaction by thin-layer chromatography. BACKGROUND REQUIRED You should be familiar with extraction‚ evaporation‚ and thin-layer chromatography techniques. You may wish to review the TLC lab from earlier in the semester! BACKGROUND INFORMATION In organic molecules‚ carbon may exist in several different oxidation states. In typical organic compounds‚ the
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Proteins (ACTIVITY 8) Gallemit‚ Lacei Machelle• • January 17‚ 2013 ABSTRACT Chromatography is one of the most useful methods of separating minute amounts of complex mixtures into the pure substances contained therein. Chromatographic separations are effected by allowing the components in the mixture to be distributed in two phases‚ one being stationary‚ and the other‚ mobile. Partition chromatography involves two main distribution systems‚ one system is composed of a gas and a liquid and
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Lab: investigating hooked law with springs Purpose: to find spring constants of different springs using the slope of a graph of change in heights vs. the weight force. Also‚ to be able to understand how spring constants change when you add springs in a series or paralle Pre lab predictions: We predicted that the graph of gravitational force (mg) as a function of stretch (delta x) would look like Data: Spring #1: y = 8.2941x + 0.0685 This table represents the different distances that
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Paper chromatography is a useful technique for separating and identifying pigments and other molecules from cell extracts that contain a complex mixture of molecules. The solvent moves up the paper by capillary action‚ which occurs as a result of the attraction of solvent molecules to the paper and the attraction of solvent molecules to one another. As the solvent moves up the paper‚ it carries along any substances dissolved in it. The pigments are carried along at different rates because they
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The goal of this lab was to create and purify DHFR from BL21 ecoli using column chromatography. To know if we isolate DHFR their will be one band at 53 KD or above 50 KD and in our desalted eluate page should have the highestOD₂₈₀ which translates to the highest enzyme activity .Column chromatography is a method used to purify individual chemical compounds from mixtures of compounds. The main advantage of column chromatography is the relatively low-cost and disposability of the stationary phase used
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must you use lead pencil‚ instead of a pen‚ to mark your chromatography paper? A lead pencil should be used instead of a pen because the ink of the pen would dissolve in the eluting solution and separate. The components of the ink could mix with the other ink/ dyes that would be tested on the same chromatogram. Thus‚ a lead pencil should be used. 2. Why should you avoid touching the surface of the paper to be used for amino-acid chromatography? We should avoid touching the surface of the chromatogram
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