Separating Substances: Identifying Food Dyes with TLC Background The color of food is an integral part of our culture and enjoyment of life. Who would deny the mouth-watering appeal of a deep-pink strawberry ice cream on a hot summer’s day or a golden Thanksgiving turkey garnished with fresh green parsley? Even early civilizations such as the Romans recognized that people "eat with their eyes" as well as their palates. Saffron and other spices were often used to provide a rich yellow color
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Full Report on Exercise 4.2 ESTIMATION OF PROTEIN CONCENTRATION BY SPECTROPHOTOMETRY And Exercise 4.3 GEL FILTRATION CHROMATOGRAPHY Joel Don M. Untalan CHEM 160.1 – 1L AY 2013 – 2014 Groupmates: Sonette Yao Kristopher Quilan Laboratory Instructor Carmelo C. Briones I. Introduction Analyzing proteins in determination of protein concentration by spectrophotometry is important. It determines to what concentration of a certain protein is in a crude sample. In this technique‚ a wide
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Cellular Biology Lab – Homework #3 Due to the week of Nov. 10-14th You may use the lab manual‚ pre-lab lectures‚ and credible internet resources‚ however you may not use your cell bio lab classmates as a resource. You will most likely see this material again on the Final and I highly encourage you to work individually and seek help from myself or your TA.Plagiarism will result in an automatic zero. 1. [15pt]In the cell bio lab‚ we use company manufactured gels‚ however you can make you own polyacrylamide
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PRE LAB REPORT Reynaldo Riboul TLC and Column Chromatography October 6‚ 2013 Table of Chemicals: Chemical Hazards Mol. Wt. Density Grams Moles Acetone Flammable‚ Irritant 58.08 g mol−1 0.791 g cm−3 2.0 g 0.0344 Hexane Flammable‚ Irritant‚ Dangerous to Environment 86.18 g mol−1 .6548 g mL−1 9.0 g 0.1044 Fluorene Very toxic to aquatic life with long lasting effects 166.223 g/mol 1.202 g/mL 0.3 g 0.00180 Fluorenone Irritant 180.20 g mol−1 1.13 g/cm3 0.3 g 0.00166
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Lab 5 The Diffraction Grating Chinua McDonald Objective: To measure the wavelength of light with a diffraction grating. Theory: The two types of diffraction gratings are the transmission and reflection gratings. They are made by ruling on a piece of glass or metal a number of evenly spaced lines with a fine diamond point. Diffraction phenomena can be analyzed in terms of Huygens’ principle‚ according to which every point on the wave front of a wave should be considered as a source
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indicated by a very pale pink color. To calculate the molarity of NaOH‚ the following equation was used MNaOH x VNaOH = MKHP x VKHP therefore the molarity was .125 M. INTRODUCTION This lab experiment covers the preparation of standard solution and the acid/base titration. The first part of the lab is to prepare a standard solution of Potassium hydrogen per. A standard solution is a solution of known concentration‚ in which it is prepared using exacting techniques to make sure that the molarity
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Analysis of Essential Oils using Gas Chromatography Lyndon Justin T. Guzman Institute of Chemistry‚ University of the Philippines‚ Diliman‚ Quezon City Date Performed: February 2; February 4‚ 2011 Date Submitted: February 18‚ 2011 Abstract The purpose of this experiment is to isolate the essential oil from eucalyptus leaves as a pure compound; moreover‚ the components of the essential oil‚ camphor and limonene‚ will be then separated using gas chromatography technique‚ identify the components by
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Plant Pigments and Photosynthesis Problem: We are going to separate and identify pigments and other molecules from cell extracts through a process called chromatography. We will also test the theory that light and chloroplasts are required for light reactions to occur. Background: Pigments are chemical compounds which reflect only certain wavelengths of visible light. This is what makes them seem colorful. Many things contain pigments including flowers‚ corals‚ and even skin. More important than
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seen at the top of the solution. Lead Acetate Cloudy solution with small specks of white precipitate. Ice cold ethanol Cloudy at the bottom and about 0.5 cm of the top of the solution was clear. Table 4: The results of Gel Filtration Chromatography Eluate Component Volume collected in mL First Blue dextrin 3.00 Second Brown cytochrome 7.00 Third Clear phosphate buffer 5.00 Table 5: The result of Electrophoresis of Amino Acids Distance
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Moment of Inertia and Rotational Motion Garret Hebert PHY 2311 Tues 1:00 garret.hebert@hindscc.edu Abstract: During this lab we will study what rotational Inertia is and how different shapes of masses and different masses behave inertially when compared to each other. We will specifically study the differences of inertia between a disk and a ring. We will use increasing forces to induce angular acceleration of both a disk and a ring of a certain mass. We will then then measure the differences
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