University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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Experiment 3: The preparation of acetaminophen (paracetamol) with thin layer chromatography (TLC) to monitor the reaction. Abstract: This experiment is to demonstrate the preparation of paracetamol and its properties. Reflux and filtration of 4-aminophenol and acetic anhydride formed the crude sample. Further analysis of dry white crystals were used to give quantitative measurements and a percentage yield of 46% was obtained. The overall conclusion is that the acetic anhydride reacted with the
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titrate the NaOH into the solution. Start the data collection to calculate the volume of NaOH is added when the solution reaches its equivalence point. Then right down the calculations and disassemble the apparatus. Discussion My lab group and I completed this experiment doing two trials. We found that both trials were concluded with similar results. In trial 1 we found that the volume of
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January 27‚ 2014 Introduction This laboratory has its first objective to familiarize with the thin layer chromatography (TLC) technique. Second‚ each student has to identify the unknown components by comparing it to one of the tree dissolved liquid analgesics: Acetaminophen‚ Aspirin and Caffeine. Finally‚ calculating the Rf value by measuring the mobile and stationary phase. Chromatography is a technique used to separate mixtures into their compounds. It’s based on a mobile phase and a stationary
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CHROMATOGRAPHY LAB Name ______________________ Date ________________ Lab # ________ Partner _____________________ Purpose: To separate a mixture into its components. To learn the technique of chromatography. Materials: filter paper 2.0 ml of vinegar Felt marker pens ruler 400 ml beaker pencil 10 ml graduated cylinder paper clip Water - H2O Safety: Wear goggles and apron during the entire lab. Clean up all materials
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The progress of the reaction was monitored in my case using two TLC plate. It first started off with the spotting of Standard benzoin and benzil which were provided in the lab and followed by the addition of the reaction mixture at once it starts changing colour/boiling‚ then at 10 and 20 mins into the reflux. Once all the necessary steps were spotted‚ the TLC plate was placed in in a beaker containing CH₂Cl₂(methylene chloride)‚ which was used as the developing solvent in this experiment. To check
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Title: Candy Chromatography Background Information and Research: Paper Chromatogrpahy is a analytical method that is used for seperating and identifying mixtures of color. There are two stages‚ the stationary phase and the mobile phase. The first is for a solid and the second for a liquid or gas. -Reference http://www.chemguide.co.uk/analysis/chromatography/paper.html Purpose: To see how paper chromatography works. Materials: · Candy with a colored coating‚ like Skittles® or M&Ms® (4 different
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dipeptide 2 Based on paper chromatogram and Rf values‚ we can conclude that the amino acid components of the dipeptide 1 sample are Glycine and Leucine (not in sequence) while for dipeptide 2 sample are Alanine and Phenylalanine (not in sequence). We compare the Rf values obtained from unknown and compare it with the standards. The amino acid identity is the one that has the lowest absolute value of difference with the unknown. In using the thin layer chromatography in the experiment‚ Sanger’s method was
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Extraction Important Peaks Concluded Compound C-5 #1 -CH‚ C=O cyclopentanone C-5 #2 -OH‚ -CH 1-hexanol D-4 #1‚2 C=O‚ -CH Cannot conclude Preparative Gas Chromatography was used to separate volatile compounds‚ which can then be identified. The injected sample becomes vaporized and was carried through the mobile column. It is usually carried by an inert gas. The gases were moved through the stationary column. The polarity of the components to the column would cause the separation of the components
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We will be testing these plants molecular and structural traits to see which one is closely related to the Botana Curus ‚ using microscopes simulated electrophoresis and much more. MATERIALS: The materials we used : 1) The Lab packet 2) Foam cups 3) Chromatography paper 4) Pen or Pencil 5) Microscope slides for species x ‚y ‚z ‚and the Botana Curus 6) Microtip dropper 7) Plant extract 8) Microscope 9) H2O Procedures:
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