LABORATORY DOCUMENTATION REQUIRED FOR DATA EVALUATION USEPA Region IX Quality Assurance Office San Francisco‚ California R9QA/004.2 AUGUST 2001 CONTENTS 1.0 2.0 Introduction General Documentation Requirements 2.1 Data Package Format 2.2 Case Narrative 2.3 Chain-of-Custody 1 2 2 2 3 3.0 Organic Analyses Documentation Requirements 4 3.1 Summary of Environmental Sample Results 4 3.2 Summary of QA/QC Sample Results 4 3.2.1 Instrument Calibration 4 3.2.2 Method
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stained with Coomassie Brilliant Blue Dye for 4 hours. SDS-PAGE Slab Gel Destaining Solution was then used for the removal of excess dye in the protein gel for 16 hours. In order to determine the Rf values of the protein bands‚ the protein gel had to be scanned using a Ricoh Super G3 scanner. Calculating the Rf values and log molecular weight values produced a standard curve with the equation of y=-1.1067x+5.2587. The antilog of the log molecular weight calculated gave a molecular weight of 79‚432 kDa
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TLC FULL LAB REPORT Objective: The purpose of this experiment was to identify compounds in a mixture by Rf values and to determine the best solvent to use. Also is the analysis of mixtures before‚ during and separation. Possible solvents: Hexane MW- 86.17 g/mol Hazards-flammable‚ harmful if swallowed Melting pt / boiling pt (degree Celsius) - 69 Density-.6548 g/ml [pic] Methanol MW-32.04 g/mol Hazards- flammable‚ eye irritant Melting pt / boiling pt- 65 Density-.7918 g/ml [pic]
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Post Lab Report Experiment 3 - Chromatography – Analyzing Analgesics by TLC and Isolation of β–Carotene by Column Chromatography Chemicals 1. Acetaminophen (C8H9NO2) 2. Aspirin (C9H8O4) 3. Caffeine (C8H10N4O2) 4. Ibuprofen (C13hH18O2) Introduction In this experiment‚ several analgesics were analyzed by Thin Layer Chromatography (TLC) and the composition of an unknown tablet was identified. We define chromatography as the separation of two or more compounds or ions by
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1. Why are spindle fibers important for mitosis? (5 points) Spindle fibers work as a path for chromosomes to travel(Campbell‚ 2009). 2. State the four bases that make up DNA. (4 points) a. (A)-Adenine b. (G)-Guanine c. (T)-Thymine d. (C)-Cytosine (Campbell‚ 2009). 3. What are the two base pairs? (2 points) a. AG b. CT 4. Answer the following questions: a. Define the term crossing over. (3 points) The phase in meiosis where chromosomes exchange segments of genetic materials (Campbell‚ 2009). b. Explain
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This experiment is concerned with identifying photosynthetic pigments found in spinach plants and determining the spectrum of light each absorbs. By using paper chromatography‚ different pigments of spinach leaves can be separated. The knowledge gained in this experiment is relevant to understanding how the process of photosynthesis works. A Real-world application for this includes the harvesting of clean energy sources‚ as scientific advances have led the way to artificial photosynthesis on the
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(Photosynthesis Lab background)
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The purpose of lab number thirteen was to examine the response of the daphnia to a chemical stimulant. Also examined was how human resting heart rates differed from the daphnia’s. Stimulants are any substances that raise the levels of activity in the body. Stimulants could be a number of things such as physical or chemical. Physical stimulus usually deal with something that affects the five senses such as sight‚ hearing these could be attributed to loud noises or too much light going into your eye
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Margaret E. Vorndam‚ M.S. Version 42-0038-00-01 Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable
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Abby Goldschmidt Honors Biology 2° Mrs. Gempel September 3‚ 2015 Daphnia Lab Results Paper Abstract The goal of the study was to observe the effects of multiple chemicals on a Daphnia magna’s heart-rate compared to a control (pond water). The different chemicals were caffeine and alcohol. The heart-rate was the main variable in this experiment. The Daphnia’s heart-rate was observed for 15 seconds and then multiplied by 4 to show its heart-rate in one minute. This was repeated 4 times for each
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