BLG1501/201/S2/2012 DEPARTMENT OF LIFE AND CONSUMER SCIENCES BLG1501 SEMESTER 2 TUTORIAL LETTER 201/2012 ASSIGNMENT 02 MEMORANDUM 2 BLG1501/201/S2/2012 Dr Monde Nyila 011 471 2294 e-mail: nyilama@unisa.ac.za Question 9.1: What is the summarised equation for cellular respiration? (6) Answer: Organic compounds + Oxygen → Carbon dioxide + Water + Energy OR C6H12O6 + 6O6 → 6C02+ 6H20 + Energy (ATP + heat) Question 9.6: Explain why ATP is required for the preparatory steps of glycolysis.(2)
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DNA bender: Cren7 & Sul7 DNA benders can introduce a bend in the DNA (Luijsterburg et al.‚ 2008). Many bends in the DNA automatically provide compaction of the DNA. Two important DNA bending proteins in crenarchaea are Cren7 and Sul7 (Driessen et al.‚ 2013). They are similar in structure‚ but they have different DNA binding regions (Zhang et al.‚ 2015). Cren7 and Sul7 can be methylated at several lysine residues (Guo‚ 2007). This PTM might be to regulate gene expression (Feng et al.‚ 2010)‚ although
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Identification and characterization of Pierisin gene in P. napi The strategy was followed to amplify the Pierisin-6 gene was given in the supplementary Fig. 1. The mRNA of pierisin-6 gene was purified from the total RNA of fifth instar larvae of P. napi‚ the conserved region was amplified using gene specific primers (supplementary table-1)‚ cloned into pGEM®-T vector and sequenced. Further‚ 3’ and 5’ untranslated regions of pierisin-6 gene were identified by 3’/5’ RACE-PCR. The amplified cDNA sequence
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Genetic engineering is a type of engineering where genes are modified to find cures‚ diseases‚ and more. Genetic engineering uses the central dogma‚ which is the idea of taking. DNA transcribing it into RNA translates it into protein and expressing it as a trait. Recombinant plasmids are when DNA fragments are inserted into a plasmid vector. The recognition site is where the plasmid gets cut by the restriction enzyme which is an enzyme that cuts a DNA molecule. Recombinant DNA is the DNA being inserted
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and the RNA World Learning Objectives: Students should be able to... • Sketch a nucleotide‚ label its three basic parts‚ and identify the 2’‚ 3’‚ and 5’ carbons. • Make another sketch showing the primary and secondary structures of DNA. • Describe the primary‚ secondary‚ tertiary‚ and quaternary structures of RNA‚ and explain in what ways RNA differs from DNA. • Explain why and how the secondary structure of DNA allows organisms to store and copy information. • Explain why RNA‚ and not
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Characterization of LIC13341 and its orthologs. The CDS LIC13341 from L. interrogans Copenhageni genome was cloned without its predicted signal peptide in pET28a expression vector and induced its expression in E. coli BL21. Induction of protein expression was evaluated using 0.5 mM IPTG where recombinant protein (43 kDa) overexpression was remarkably observed (Fig 2A). In order to further characterize LIC13341‚ polyclonal antibodies were generated in BALB/c mice against r-LIC13341. To check its
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In 1953‚ Francis Crick‚ along with James Watson‚ came up with the idea that DNA was in a double-helix structure. Well‚ they were the ones that got the Nobel Prize in 1962. A huge part of the discovery of the double-helix structure should go to Rosalind Franklin. But‚ since she was a woman‚ she did not receive much recognition for her work. In 1952‚ Raymond Gosling‚ under Franklin’s guidance‚ took an X-ray diffraction image of DNA. This image proved that DNA was a double-helix. The experiment is posted
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The Effect of Srb-16 and F35B1.3 RNAi Gene Treatment on Chemosensation in Caenorhabditis elegans through Observation of Chemotaxis. Abstract: The study of sensory systems in humans is a vital area of study to further pursue our knowledge of the mechanisms occurring in the nervous systems. However due to the complications surrounding human testing‚ model organisms such as C. elegans worms can be utilized as a comparison to create paradigms relevant to human sensory systems as the genetic basis
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Nowadays‚ the interactions between the epigenomics and transcriptomics are widely studied. The interplay between the chromatin and transcription factors is very complicated (Angelini & Costa 2014). Chromatin is composed of DNA that is wrapped around nucleosomes to form domains of accessible (open) and inaccessible (closed) chromatin. The histone proteins regulate the chromatin accessibility. Accessible chromatin can facilitate association of transcription factors to DNA (Angelini & Costa 2014). Some
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II-Leukocidin genetic organization and genome distribution The genetic architecture of the S. aureus leucocidins is widely conserved. F and S subunits of a certain toxin pair are transcribed from specific single promoter. The open reading frame of the S subunit immediately come before The open reading frameof the F subunit‚ with as little as a single nucleotide separating each open reading frames (Fig.1). The only known exception to this rule iwas in gamma-hemolysin locus. 1-Gamma-hemolysin:
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