then cleaned my work area. Transferring one capsule of L. acidopholis into a tube of MRS broth using the aseptic transfer technique then marked a line on test tube to record sediment. Labeled tube of nutrient broth S. epidermidis‚ then using a sterile swab obtained sample of bacteria from skin then transferred using the aseptic transfer technique into the sterile media. Incubated both specimens for 48 hours observed and recording results of growth at 24 and 48 hours. After observing final growth
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Our main goal was to test organisms’ resistance and susceptibility to bacteria‚ and whether the cell wall of bacteria exposed the membrane to pathogen or protect it from pathogens. I thought P. aeruginosa was gram-positive and therefore it would be susceptible. That was my hypothesis.We divided antiseptic agar plate into quadrants and divided antibiotics agar plate into six areas. We wrote the organisms’ name‚ date‚ and the temperature on the bottom of the agar plates. For antiseptic‚ our group used
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The properties of dibenzalacetone make it a great substance to be used when protecting the skin from the sun. In the experiment performed‚ dibenzalacetone was created from an aldol condensation of 2 portions of benzaldehyde and acetone in sodium hydroxide. The reaction that occurred in this experiment is known as a specific aldol condensation called Claisen-Schmidt. In the reaction‚ the carbonyl of benzaldehyde reacts quickly with the acetone anion to create a hydroxyketone that dehydrates in the
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04/29/2013 G-Unknown code Gram Positive-Micrococcus luteus Gram Negative- Klebsiella pneumoniae Unknown Paper The unknown project was a very good realization of me and my partner going out by our selves. The very first day of the Unknown project I was acquainted with Denise who was very friendly and was very nice as far as assisting me in the project. The first day we were introduced too various different forms of the unknown such as broth‚ Blood agar plate‚ MSA plate
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The human cardiovascular system consists of the heart‚ the blood‚ and a system of transporting vessels. A human heart has four chambers: a right and left atrium and a right and left ventricle. The fist-sized heart sits in its own sac (the pericardium) in the middle of the chest under the sternum. In most people‚ the apex of the heart points to the left. There are two circuits of simultaneous blood flow in humans: a pulmonary circuit and a systemic circuit. In the pulmonary circuit‚ the right
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completed in order to identify this unknown sample. First‚ the plate was analyzed for its hemolysis characteristics. Afterwards‚ a Gram stain was performed from a sample of the agar plate and the slide was viewed under the microscope. Once‚ the microscopic visual was captured‚ a catalase test followed. Next‚ for further data‚ MSA results were recorded‚ along with antibiotic observations of demo plates. Lastly‚ with the information gathered‚ it was identified that the unknown sample was Streptococcus salivarius
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Autumn White Biology 258-05 November 26‚ 2012 Unknown #19 Enterbacteriaceae Enterobacter aerogenes ABSTRACT The objective of this report was to identify an unknown microorganism through several differential media tests. Over the course of a couple weeks‚ ten tests were performed. First‚ a gram stain was performed‚ indicating the bacterium was gram negative. An aerotolerance test determined that the bacterium was a facultative anaerobe. Next‚ a negative result in the
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to help move the reaction towards the products motioned above. To determine the successfulness of the experiment and the amount of product(s) recovered‚ IR spectroscopy‚ Gas Chromatography and percent yield calculations were used. Equations and Structures 6. References 1. "18. Dehydration of 2- Methylcyclohexanol." Organic Chemistry Lab 2040L. XU Chemistry‚ n.d. Web. 12 Feb. 2013. 2. "Material Safety Data Sheet 2-Methylcyclohexanol." Http://www.coleparmer.com/Assets/Msds/97403
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a. The bacteria that causes botulism are found in soil‚ water‚ animal guts and dung. When the spores exposed to low oxygen and certain temperature‚ they produce botulinum toxin‚ able to germinate‚ metabolize‚ food sources through an aerobic respiration to produce toxin. The toxin blocks the release of acetylcholine from motor nerve endings causing paralysis symptoms. The botulism either food born‚ eating contaminated home-canned foods where the fermentation takes place. or through abrasion in the
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Halobacterium Lab Investigation Mason Pirio 12/12/13 Period 5 Table Group 3 PS 2- Experiment Design PS 3- Conclusion Writing PS 13- Nitrogen Cycle Abstract- In this experiment we tested to see how well halobacterium grows in different levels of salinity. We found out that the higher salinity in the growth medium the better the halobacterium grows. Background Information- Halobacterium is a bacteria that is found in the great salt lake. Halobacterium is an extremeophile which means it thrives
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