Lecturer’s name: Mr. Paul Davidson Discussion The name of enzyme involved in this experiment is salivary amylase‚ which presents in our saliva. Salivary amylase catalyses the breakdown of complex sugar‚ such as starch‚ into a simple sugar‚ such as glucose. In this experiment‚ the optimum temperature for salivary amylase to function is 37˚C. Above the optimum temperature‚ the kinetic energy in the substrate and enzyme increases. The number of collisions between
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Bio Lab Report In part II of the Organic Compounds in Foods lab‚ we to examining whether or not the starch in the saltine cracker can chemically break down in sugar with the presence of salivary amylase‚ which is an enzyme found in your mouth which assists in digestion. My group and I were instructed to obtain a saltine cracker from our teacher and chew on it for approximately 2 minutes. After concluding the chewing‚ we then grabbed a beaker and spit our slimy and chewed cracker into it. Subsequently
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remained on the white tile. It was opaque. Presence of reducing sugar ‚ absence of starch Solution B Benedicts test: Blue colour remained the same. Abundant amount of blue solutions remained the same and it was opaque. Iodine test: Yellowish-brown colour turned to blue-black. Moderate amount of blue-black precipitate was left on the white tile. It was opaque. Absence of reducing sugar ‚ presence of starch Table 2 : Tube Contents Temp (℃) Benedict’s Test – Colour Observation
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most useful substances for many living organisms: Carbohydrates. Carbohydrates are used for numerous processes in organisms from acting in important roles of cellular recognition in the human body to storing chemical energy in plants in the form or starch. The roles of carbohydrates in the human body alone are numerous; providing energy for working muscles‚ providing energy for the regulation of blood glucose‚ preventing proteins being used for energy‚ breaking down fatty acids and playing a part
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12 Experiment No. 12 Aim- To study the effects of temperature on the enzymatic activity of salivary amylase on substrates starch. Materials required- Test tubes‚ beakers‚ pipettes‚ funnels‚ thermometer‚ cotton‚ starch‚ iodine‚ potassium iodide‚ sodium chloride‚ thermocol box‚ buffer solution of pH 6.8 & match box. Principle- Salivary amylase is an amylolytic enzyme found in saliva released from salivary glands. All the enzymes show optimum enzymatic activity around 37° C which is our body temperature
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lab‚ I will learn how the complex digestive system breaks down substrates such as fats‚ proteins and carbohydrates. Activity 1: Assessing Starch Digestion by Salivary Amylase Data: Chart 1 – Salivary Amylase Digestion of Starch Tube # 1 2 3 4 Additives Amylase‚ Starch Amylase‚ Starch Amylase‚ DI Water DI Water‚ Starch pH 7.0 buffer pH 7.0 buffer pH 7.0 buffer pH 7.0 buffer Incubation Cond. Boil‚ Inc. 37 deg.C 37
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glands like salivary glands‚ gastric glands‚ pancreatic‚ intestinal glands and liver. Also‚ the four layers of the digestive system are mucosa‚ submucosa‚ muscularis and serosa. The digestive system is regulated both by the Autonomic system and the Enteric nervous system. The major functions of the Digestive system are; passage and breakdown of food‚ destruction of ingested bacteria‚ viruses and toxins‚ Absorption of nutrients‚ Elimination of
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Iodine is a test for starch while Benedict’s solution is a test for reducing sugars. When solution A is tested by benidicts test‚ the clear blue solution changed to a little reddish and brick red precipitate is formed.this result show that solution A is a reducing sugar. When carried out iodine test with solution A‚ the colourless solution remain unchanged . this tell us that starch is absent is solution A. When solution B is tested with Benedicts test‚ the clear blue solution remain unchanged‚ we
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Activity 1: Assessing Starch Digestion by Salivary Amylase. 1. At what pH did you see the highest activity of salivary amylase? 7.0 Why? because that is when the salivary is most effective and it breaks down carbohydrates 2. How do you know that the amylase did not have any contaminating maltose? because the sample didn’t change color. Negative sugar/IKI test. No change from original blue bright color. We used a negative control to see if there is any amylase in the maltose 3. What
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heavy-metal salts upon the activity of salivary amylase. Results: A. Enzyme Concentration Test tube | Drops of Enzyme | A-1 Starting Time | A-2Time Blue Color Fades | A-3Time for Starch Hydrolysis | 1 | 1 | 9:07 am | 9:42 am | 35 minutes | 2 | 5 | 9:07 am | 9:37 am | 30 minutes | 3 | 10 | 9:08 am | 9:33 am | 25 minutes | 4 | 15 | 9:08 am | 9:28 am | 20 minutes | 5 | 30 | 9:09 am | 9: 24 am | 15 minutes | Figure 1: Enzyme Activity (Time for Starch Hydrolysis) vs. Enzyme Concentration
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