Hypothesis: the higher the concentration of hydrogen peroxide the faster the reaction will occur and the more foam will be produced. Aim: The aim of this investigation is to determine how the concentration of hydrogen peroxide effects the intensity of the reaction. Variables: Independent- The concentration of hydrogen peroxide. Dependant - The intensity of the reaction. Constants – The temperature‚ the amount of liver‚ pH level‚ test time‚ Materials: Solutions of hydrogen peroxide varying
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they come into contact with atmospheric oxygen. As the pH of solution increases‚ so does the degree of browning of the apple slice. Low pH solutions will cause the enzyme to be inactivated and therefore cause the degree of browning to decrease. As the pH increases so does the degree of browning‚ because the pH becomes closer to the enzymes optimum pH level. Independent Variable: The pH solutions that the apple pieces are placed into. Range of pH levels to be tested is 3‚ 6‚ 8‚ 10‚ and 12. Each solution
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Enzyme Lab Daniyal Abdali (Rachel Lee) (Sarina Dolch) SBI 3UI Mr. Vrabec October 20‚ 2009 Test #1 * Add a small piece of cracker in test tube #1 and add Lugol’s solution. Observation #1 * The cracker turned a black colour when the Lugol’s solution was added to it. This was a positive result‚ meaning that the cracker contains starch. Test #2 * Add a bigger piece of cracker in test tube #2‚ add 5 mL of Benedict’s solution‚ place in a boiling water bath‚ and record observations
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Division Ave. High School Ms. Foglia AP Biology Metabolism & Enzymes AP Biology 2007-2008 Forms of Energy Energy Is the capacity to cause change Exists in various forms‚ of which some can perform work Kinetic energy Is the energy associated with motion Potential energy Is stored in the location of matter Includes chemical energy stored in molecular structure AP Biology 1 Division Ave. High School Ms. Foglia AP Biology Energy
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The enzyme tyrosinase was successively extracted by combining a homogenate of a potato and sodium sulfate with ammonium sulfate. Tyrosinase was successfully extracted by taking advantage of solubility properties of certain proteins. A standard curve was generated indicating dopachrome absorbance values through the use of a spectrophotometer and a computer graphing program. A spectrophotometer was used to measure either the amount of light that passed through a solution (transmittance) of the amount
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Lab report nº4 The aim of this experiment was to observe the change of enzyme reaction with different concentration of solution. For this experiment we used potato enzymes (catalase) and hydrogen peroxide in concentrations of 100%‚ 80%‚ 60%‚ 40%‚ and 20% According to P.George: “When catalase is added to hydrogen peroxide‚ there is an initial rapid evolution of oxygen which lasts for about two minutes‚ depending on the peroxide concentration. After this‚ oxygen is given off at a steady rate which
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Fungal Amylase and hydrolysis of Starch Abstract This experiment consisted of setting up a control group of starch in various temperature and then placing both fungal amylases and bacterial amylases in a mixture of starch and placing the solution of amylase and starch in various temperatures of water. After a certain amount of time- different amount of time needs to be used in order to have reliable results- iodine is added in a well on spot plates‚ then two drops of the mixture of amylase-starch
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1.7 Factors that affect the activity of an enzyme It is important when working with enzymes to understand basic enzymatic theory behind them when selecting conditions to measure the activity of the enzymes. The factors that are known to affect the concentration of enzymes are temperature‚ pH‚ concentration of enzyme‚ concentration of substrate‚ buffer type and concentration‚ the presence of any inhibitors and cofactors (Worthington-biochem.com). 1.7.1. Temperature With most catalysed reactions‚
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Six Factors Affecting the Performance of an Enzyme Enzymes are considered catalysts; substances that increase the rates of reactions. Enzymes are responsible for thousands of metabolic processes that involve the sustainment of life‚ one common one is simple food digestion. Without enzymes‚ digestion would occur too slowly for life to continue. Enzymes maintain a protein structure consisting of one or possibly more than one polypeptide chains of defined primary structure‚ and take up a characteristic
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Enzymes are responsible for many crucial processes in living cells since they accelerate chemical reactions which would occur too slowly‚ or would lead to different products without their contribution. Enzymes are biocatalysts that usually show high affinity to a specific substrate under particular environmental conditions. The binding of the substrate and catalysis take place at a specific small region‚ around 10 amino acids‚ in the enzyme known as active site which usually represents a hydrophobic
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