Abstract Over a two week period of time in the laboratory‚ we experimented and tested the reaction rate of a peroxidase enzyme and the factors that affected it‚ both positively and negatively. The purpose of these experiments was to probe and manipulate the activity of the enzyme peroxidase by varying temperature‚ pH‚ the amount of enzyme compared to the substrate and the effect of hydroxylamine. Peroxidase activity is expressed when the potato extract is subjected to stresses such as low temperature
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Jeopardy questions (2nd half) Old stuff 1. Draw and correctly label a graph to illustrate the principle of maintenance. 2. Who would have the higher blood lactate (all other things being equal): a. John who has more 2-3 DPG or b. Jay who doesn’t? Why? (A logical explanation must be provided for any marks) 2. What does M type LDH do? Nervous system 1. What part of the neuron is damaged with multiple sclerosis? 2. The resting membrane potential in neurons is generally in what range?
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Identification of unknown a-Amylase through testing different temperatures and pH values to detect the absorbance of maltose. Introduction: Enzymes are biological catalysts‚ mainly proteins for this experiment‚ generated by an organism to speed up chemical reactions. They have active sites on which the substrate is attached‚ and then broken up or joined. For this experiment we are going to work with the enzyme a-amylase. Amylase is an enzyme that breaks starch down into sugar. Amylase is present in human
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Effects of Angelica keiskei (Ashitaba) Leaves on the Salivary Bacterial Count and Salivary Flow Baldemor‚ Levee M. Dalida‚ Racy Detaro‚ Isabella Cherr K. Espineli‚ Felipe Rafael B. Perdido‚ Lanz Gerard O. Sta. Maria‚ Danica C. Teodosio‚ Kim Crizandra D. Zantua‚ Jazelle Christine G. Introduction Angelica keiskei (Ashitaba) is a lush green plant. The name “Ashitaba” means “tomorrow’s leaf” aptly termed due to its ability to reproduce green stem
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Effect of different temperatures on amylase activity. Literature review This study is an attempt to follow the activity of amylase because it has a major role in the life of living organisms and is found abundantly in them. Amylase is a catalytic enzyme which hydrolyzes starch into maltose and dextrin at a certain temperature (Biology.kenyon.edu‚ 2015). In plants such as fruits and vegetables carbohydrates are referred to starch which is polysaccharide and is converted into disaccharide and eventually
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The main focus of this experiment was to find the relationship between temperature and the enzyme activity of amylase. This was achieved by attaining amylase enzyme‚ starch solution and potassium iodide (determines if enzymes hydrolyses the starch solution)‚ water bath and a hot plate. The temperatures used for this experiment were room temperature‚ 37oC‚ 60oC‚ 80oC‚ and 90oC. The hypothesis developed was that as the temperature increased‚ so will enzyme activity. Therefore‚ the ability of the
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Enzymes are biological molecules (proteins) that act as a catalyst and help complex reactions occur everywhere in life‚ for example a piece of steak that is being digested into energy. Molecules found at the beginning of the process are called substrates‚ and these enzymes exchange them into differing molecules known as products. Nearly all-metabolic processes in a cell need enzymes in order to function at rates that are fast enough to sustain existence. Those who are lactose intolerant are simply
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Natural enzymes are proteins that catalyze biological reactions by lowering the activation energy of the reaction without being altered during the process. The enzyme used in this experiment was the β-galactosidase purified from E. coli. This enzyme hydrolyzes lactose and turns it into galactose and glucose. Since it is difficult to assay the activity of β-galactosidase‚ we will be using the artificial substrate‚ o-nitrophenyl-β-galactoside (ONPG) instead of lactose. ONPG is an analog of lactose
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W. HENRY et al.: Polytene Chromosomes of S. (Nevermannia) praelargum UNCORRECTED PROOF Polytene Chromosomes of an Indian Himalayan black fly Simulium (Nevermannia) praelargum (Diptera: Simuliidae) Willie HENRY1‚ Subrata Kumar DEY2‚ Rakesh VARMA1‚ Sachin THAPA1‚ William S PROCUNIER3 1 2 P.G. Department of Zoology‚ Darjeeling Government College‚ Darjeeling‚ West Bengal 734101‚ India School of Biotechnology‚ West Bengal University of Technology‚BF-142‚ Sector 1‚Salt Lake City‚ Kolkata 700064
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To investigate the affect that change in pH levels has on a particular enzyme‚ in this case amylase. Hypothesis: In this investigation I expect as the pH reaches the optimum level‚ the rate of reaction will be fastest‚ compared to other pH levels. It is also suspected that after the enzyme has reached optimum level the enzyme activity will decrease. Through further study of the optimum level of amylase I found that the enzyme usually has an optima pH of 8. It is known that the pH of an enzymes
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