Transformation1 lab manual. An Escherichia coli bacterial reference plate was used to obtain colonies which were resuspended into a CaCl2 solution that was previously kept on an ice bath. The rainbow transformation mixture containing the plasmid DNA was then added to half of the E. coli cells. These cells were later placed into a water bath set to 42ºC and “heat shocked” to promote the entrance of DNA into the cells. Moreover‚ a Recovery Broth was added to the sample and the sample was left undisturbed
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6 M HNO3 was added. Then 5 drops of 0.1 M AgNO3 was added and the solution was centrifuged for 2 minutes. Then the supernatant was discarded. Then 4 drops of 6 M NH4OH was added to the precipitate. To solutions where the precipitate dissolved the sample was acidified with 6 M HNO3. If the precipitate reforms then it contains chloride. All observations were recorded. Next add 10 drops of sulfate solution to a new centrifuge tube and several drops of 6 M HNO3 solution was added until the solution turned
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their abilities to develop and adjust to altering environments. That is why it is so significant to be competent to identify microorganisms. This study was conducted by applying techniques and experiments cultured in microbiology lab for the identification of the unknown microorganism. Introduction: Identification of bacteria is significant because they are not only beneficial but potentially hazardous as well. The identification of bacteria can lead to breakthroughs in healthcare regarding treatment
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Kristen Jones Section: Lab Report 5: Lymphatic System – Lab Manual Pages 103-112 Relevant Websites to Help You with This Lab – Be sure to review these Websites before and during the completion of this Lab. • Antigen/Antibody Test Results • Human Anatomy Online : The Lymphatic System • Immune Response Animation • Lymph Node Animation Review Your Lab Summary: What were the most significant 2 or 3 concepts you learned while doing this Lab? Exercise 1: Microscopic Anatomy
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MICROBIOLOGY 3444-007 Gram Negative Unknown Unknown Bacteria #14 LeNaiya Kydd 4/2/2014 Abstract In order to be able to identify the unknown organism that was given to us‚ we had to conduct a number of different tests. These biological tests are used because they help us be able to identify the properties of the unknown we have and be able to compare our observed results with actual results of all the potential organisms. When all of the data of the test are put together it is easy to
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The purpose of the following study is to determine where the two unknown bacteria acquired in Microbiology lab should be classified in regards to temperature‚ pH level‚ and osmoregularity. It is important to classify bacteria in order to identify them. Identification of bacteria is important because they are not only useful but potentially dangerous as well. The identification of bacteria can lead to breakthroughs in healthcare regarding treatment of old and new diseases alike. Identifying bacteria
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Evaluate how the name of a muscle can distinguish its location‚ action‚ shape‚ and function. Select five different muscles to make this distinction. Descriptive terms are used to name skeletal muscles. Some names give the location in the body. The temporalis muscle is attached to the temporal bone in the skull. The brachialis muscle is attached to the humerus bone‚ but brachial refers to the main artery in the arm. Some muscles are named for their origins and insertions‚ like the genioglossus
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SAMPLE REPORT: The ‘Stylish’ Report i) ii) Look at the assignment instructions below Now read the sample report. Read the accompanying comments on the side as you go. iii) Is this is a well-written report in your view? Could it be improved in any way do you think? ASSIGNMENT 2: The Stylish Report Background Colin Coif and Pamela Perm are the owners and operators of a hairdressing business Stylish specialising in ‘high end’ quality hairdressing services. Currently they operate two salons in
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The purpose of the unknown bacteria lab assignment was to select an unknown bacteria culture and‚ through a series of metabolic tests‚ identify which bacteria genus resided in the pure culture received. A nutrient broth inoculated with bacterial culture (numbered 45‚ henceforth referenced as U45) was selected and a streak plate was made to isolate a pure culture for use throughout the assignment. From the streak plate‚ several slides were made to determine the morphology of unknown 45. A Gram stain
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As a result of the tests ran‚ I was able to identify the unknown bacteria. For Unknown A‚ I ran six tests. I first isolated the bacterium from the second bacterium and found a clear growth (Table 1‚ Figure 1). Secondly‚ I ran a gram stain and found a gram positive‚ cocci bacterium (Table 1‚ Figure 2). Third‚ I ran a catalase test in which was negative (Table 1). From here‚ I determined a starch hydrolysis test would be necessary to distinguish between different bacteria. The result was negative (Table
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