Exercise 3.9 Endospore Stain (Differential Stain) Eula Lewis Bio 2921 February 27‚ 2013 Zere Ezaz‚ Ph. D. Objective: 1. To learn to endospore stain 1. To distinguish endospores‚ free spore‚ and vegetative cells Theory: A dormant form of bacterium that allows it to survive poor environmental conditions are called endospores. Their tough outer covering is made of keratin and is also the reason they are resistant to heat and chemicals. The strength of keratin also makes it resist
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Endospore and Capsule Cell Staining Allison Lui Partner: Mary Chagin BIOL-235 W07 Dr. Runco 2/10/15 Introduction The purpose of this lab is to learn what endospore and capsules are‚ and how to identify them under a microscope using capsule and endospore staining methods. Capsules are found only in select bacteria‚ and serve a protective purpose. Made out of sugars and proteins‚ they are antiphagocytic‚ which prevents other cells to engulf the bacteria through phagocytosis. It
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Case IV-3: IT Infrastructure Outsourcing Schaeffer (A) Schaeffer Corporation must have improved information technology services in order for them to achieve their ambitious growth goals. Schaeffer’s IT is not one of their core competencies and they are able to save some money by outsourcing and expending into additional countries and acquiring these new companies will require extraordinary IT support efforts. Schaeffer Corporation has five challenges associated with their decision to outsource
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cell wall. The dye will be locked in the spore wall‚ which has peptidoglycan deeper in the walls. The keratin forming the outer portion of the endospore wall resists dye. The heating of the bacteria will make the spore wall more permeable to the malachite green‚ and it then attaches to the peptidoglycan. Thus‚ we can detect the bacteria that have the endospore based on this test. On a clean glass slide a drop of distilled water will be added to the slide. A smear will be prepared on this droplet by
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future use in various fields. -Simple stain helps with determining the sizes‚ shapes and arrangements of microorganisms. -Gram stain assists in identifying the type of microbes‚ whether they are gram positive or negative‚ which in turn tells us what kind of cell wall the microbes have. -Endospore stain tells whether microorganisms make endospores. -Acid-fast stain is helpful in recognizing the existence of mycolic acid in cell walls. -Capsule stain reveals the existence of capsules.
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Gram Stain: The important part of this experiment is being able to determine a bacterium based on its cell wall structure. It also helps indentify if the unknown organism is a Gram positive or Gram negative. This is the initial step that must be taken before any other lab procedure may continue on to ensure the purity is present‚ the arrangement of the cells‚ and the shape the cell has. The staining of the cell starts off with using the primary stain‚ Crystal Violet which is a purple color‚ to begin
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Permanent Stain I stood peeking through the small living room doorway and watched my mother beat my sister. My mother had a full grip of her hair in one hand‚ and a thick‚ coiled wire in the other. She took a blow to her delicate skin‚ releasing all of her held in anger. I see my sister curled up on our dirty‚ kitchen floor‚ trapped between our wooden door and filthy fridge. I hear her cry as if she cried her lungs out for a stop to the blows she’s forced to take‚ and for a stop to the thought
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1: Victoria ’s Ugly Stain Introduction: The purpose of this assignment is to give you an opportunity to apply some of the critical reading strategies you have evaluated. Resources: Go to Writing on the Run! to review the “Writing Process.” You may also want to review your English 11 Introduction Assignment for a tutorial on how to write a paragraph. Instructions: Complete the assignment by following the steps outlined below. STEP A: Read the article “Victoria’s Ugly Stain”; then answer questions
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Paper #4 At some point in most children’s lives they create an imaginary friend. This imaginary friend is created so the child feels special attention that they may not get from the people around them. In the stories “Charles” by Shirley Jackson and “Doby’s Gone” by Ann Petry‚ both Sue and Laurie lack attention from either their parents or peers. Laurie is led to create Charles because of his lack of attention from his parents. By creating Charles‚ he is indirectly getting attention without
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Conclusions: We learned that some nasty stains can come out with some unorthodox products. We used on our stain Isopropyl alcohol (rubbing alcohol)‚ bleach‚ and shout. The product that did the best at pulling out stains was the Isopropyl alcohol. Isopropyl alcohol is a clear‚ colorless‚ flammable‚ mobile liquid‚ (CH3)2CHOH‚ used in antifreeze compounds‚ in lotions and cosmetics‚ and as a solvent for gums‚ shellac‚ and essential oils. It did great job at pulling stains right out of the cloth. This products
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