If there is any update‚ it will be posted no later than this Saturday (2/8/14) Identify the following individuals’ major contributions to science Ehrlich – magic bullet Hooke –cell theory and microscope Jenner - cowpox Lister- disinfectant (phenol) Koch- kochs postulate Lancefield- immunology to classify via serotypes Linnaeus- nomenclature Pasteur- pasteurization‚ vaccine‚ microbes in nonliving matter‚ aseptic techniques‚ fermentation. van Leeuwenhoek- described live organisms with Hooke’s microscope
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desert‚” but rather‚ solvents are dry in that they do not need water to be effective. In other words‚ these solvents used today are non-aqueous. In Drycleaning: Technology and Theory‚ authors Martin and Fulton define dry cleaning as “the cleansing of textiles in an organic solvent.” (Martin and Fulton‚ 1) The authors note that the process birthed in the middle of the 19th century in Paris‚ France‚ when camphene‚ an oil used for lamps‚ was spilled onto a gown accidentally. Consequently‚ the gown seemed
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following sequences exhibits increasing size? Viruses to bacteria to protozoa - Which one of the following would be consistent with the idea of spontaneous generation? Sick people give rise to microorganisms in their body Chapter 3 - The gram stain technique is valuable in distinguishing: different types of bacteria One of the below is wrong - permit eukaryotic motility: Flagella - are involved in group translocation: Chromosomes - Contain components of the electron transport chain for
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their advantages and disadvantages? 7.* Define the following terms: focal point‚ total magnification‚ resolution‚ refractive index‚ parfocal. 8. Identify the parts of the light compound microscope. What is the function of each? 9. What is a simple stain? Give one
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Plasmodium‚ Trypanosoma Algae: Diatoms‚ Spirogyra‚ Volvox Helminths: Tapeworm‚ fluke Fungi: Methylene blue stain of yeast cells (Saccharomyces)‚ sporangiospores of Rhizopus‚ conidiospores of Penicillium Staining Labs (3-6‚ 3-7‚ 3-8‚ and 3-9) – Identify and correctly interpret the following: Lab 3-6: Gram stain Lab 3-7: Acid Fast stain Lab 3-8: Capsule stain Lab 3-9: Endospore stain Metabolic Tests: Lab 5-1: O-F Glucose (aerobic versus fermentative organisms) Lab 5-2: Phenol Red broth for
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Aseptic Technique and Culturing Microbes‚ Lab #3 Purpose: The purpose of the this experiment is to learn to transfer microorganisms to a nutrient medium to promote growth using aseptic techniques. which are a method used to prevent unwanted organisms from contaminating a medium. We will also learn about different types of culture media‚ including MRS broth and nutrient broth‚ learn about oxygen and temperature requirements for microbial growth and how to control microbial growth. Procedure:
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plates were properly inoculated and incubated at 37C for 24- 48 hours‚ and two separate morphologies were then observed and recorded‚ unknown A and unknown B‚ a Gram stain was then performed for each unknown. After determining the Gram reaction‚ alternate staining procedures were performed as needed. Unknown A required further endospore staining‚ whereas unknown B required no further staining procedures. Once all staining methods were complete‚ specific biochemical tests were performed. The biochemical
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bleach solution -Gloves -S. epidermidis sample -Goggles -Thermometer -Apron -Test-tube rack -Candles -Slide box with blank slides -Cover slides -Broth MRS-9ml -Broth‚ Nutrient -Lactobacillus acidophilus -Sterile swab -Gram stain solution #1 crystal violet -Mask -Inoculation loop Procedure: -Set up incubation site -Determine medium type -Generate microbial cultures -Observe my microbial cultures * for a more detailed outline of procedures and step by step process
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Simple Stain- Uses 1 Stain 1. Acid Stains (- Charge)- Stains Background- Nigrosin‚ India Ink and Congo Red 2. Base Stains (+ Charge)- Stains Cell- Methylene Blue‚ Crystal Violet‚ Safranin Differential Staining Techniques- Any Staining Technique using 2 or more stains is differential. It allows us to differentiate between parts. 1. Gram Stain- Two Stains‚ PLUS Reagents- Distinguishes Chemical Composition of Cell Wall PG only (+ Purple) or LPS and PG (- Pink) Primary Stain- Crystal
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Staining and Identifying Unknown Bacteria Introduction: The microbiology lab up to this point has been used to teach the students how to stain and identify bacteria. There are several types of staining through which the bacteria can be identified based on the color and shape. The staining methods used in the lab are Gram Staining‚ Capsule Staining‚ Endospore Staining‚ and Acid Fast staining. One of the most significant method of staining is the Gram Staining‚ as it is highly dependent (McCarthy
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