Introduction: The gram stain is one of several laboratory procedures that can be used to narrow down the identities of unknown bacteria. Bacteria have three different shapes; cocci‚ bacilli‚ and spirilla. Since bacteria pretty much have the same reflective index as water‚ a bacteria cell must be dyed so that these shapes can be seen. Materials: Petri dish Dropper Cleansing solution Slides Bibulous paper Inoculation loop Crystal violet dye‚ Iodine Acetone Safranin Water from the sink
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Part B: Gram Stain Preparation: Watch the following video: The Gram Stain (4:15 minutes) Procedure: Include the answers to the questions below in your lab report. Lab Questions: This procedure is used to separate what two types of bacteria? This separation is based on differences in what physical trait? What is the iodine used for in this staining technique? Why is it important to heat fix the bacteria prior to the staining procedure? If you had a bacteria sample that you knew to be gram
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The acid-fast stain is performed on samples to demonstrate the characteristic of acid fastness in certain bacteria. Acid fastness is a characteristic that is shared by just a few organisms‚ so staining to determine if organisms possess this trait is useful in microbial identification schemes. The Ziehl-Neelsen method has endured as a reliable and effective way to demonstrate the acid-fast bacteria. Materials: 18-20 nutrient hour agar slant culture of Staphyloccus aureus 4 day old nutrient
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The insufficient inward base Francis Schaeffer contents was the resulting factor to the fall of Rome is plausible as the Roman Empire had become too large to govern adequately and efficiently. “In many ways Rome was great‚ but it had no real answers to the basic problems that all humanity faces” (Schaeffer‚ 2005‚ p. 20). These basic problems included the means to provide political‚ economic and military stability. Shaped by the culture and thinking of Greece‚ Rome attempted to build upon the Greek
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Batangas: A world class and eco-friendly tourist destination in this part of Southern Luzon I. VISION Batangas Province: A world – class destination with ecologically balanced and sustainable development‚ state-of-the-art infrastructure‚ home of God-loving and family-centered people‚ governed by dedicated and professional political leadership‚ committed to one vision and shared responsibilities geared towards better quality of life for all Batangueños. II. MISSION Batangas Province is committed
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Sodium Bicarbonate as a Stain Remover for Grease Abucay‚ Larry Fernandez‚ Ivan Gonzalez‚ Angelo Macabuhay‚ Michaelangelo Manlangit‚ Luis Alfonso Morales‚ Don Niel LSGH 2013-2014 The Problem and Objective: Stain removers in the market today have multiple health hazards that could endanger our health. Dangerous chemicals can cause irritation‚ health problems‚ dangerous fumes and other complications. The group aims to find a new alternative material for the removal of stains. The group also aims
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Averrhoa bilimbi L. (IBA / KAMIAS) Extract as Stain Remover l. Introduction A. Background of the study Stains are the most common problem of people living now a day because of too much works. We can remove it using a commercial stain remover‚ but these are harmful for it contains substances that are bad for ourselves and the environment. The researchers heard about the use of kamias as stain remover and they wanted to see if this will be effective. B. Statement
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4: Cell Structures and the Gram Stain Purpose: The purpose of this lab is to familiarize the student with the use of a compound microscope and the Gram Stain test. Students will review a brief video and slides to introduce the compound microscope. Students will also view a video about the Gram Stain procedure and then answer questions related to it. Hypothesis If I look at the slides I will see where the cell wall is and what lies inside it and in the Gram Stain if I watch the video I will learn
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When I received my slant culture‚ I immediately made a streak plate and started the Gram Stain protocol1. In the first two slides‚ I made and stained‚ my results were ambiguous. Without putting the slide into the microscope‚ I noticed one was pink and the other purple. To resolve it‚ I discarded my slides and started again and was extremely careful in proceeding the Gram Stain. I concluded that my unknown A was gram-negative bacilli. Using the dichotomous key for gram-negative bacilli I started
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criminality has been a major problem and the most debated topic of interest by theorist on the grounds of why crime is committed‚ who is more prone to commit crime‚ and what prevents people from committing delinquent acts. The formulation of the Stain theory and Anomie Theory by Robert Merton give insight on the many question left unanswered about the reason for criminality. The Strain Theory decodes the mechanics of crime committed as being social structures in any given society pressuring its
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