biotechnicalfieldsThe cell wall is the basis for classification of bacteria according to the Gram stain. Accordingto the chemical make up of bacterial cell wall‚ a staining procedure‚ Gram stain‚ helps usclassify bacteria into two subgroups‚ The cell wall can stain either positive or negative‚depending on its chemistry. If the bacteria stains positive it will retain a purple/blue color. If the bacteria stains negative‚ the bacteria will not retain the purple/blue color‚ but rather have a pinkish/red color
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Questions A. What is a differential stain? How is it different from a simple stain? the use of two or more stains to categorize cells into groups. simple stain uses one dye and shows only structure‚ morphology and size. a differential stain uses two or more dyes and is used to show morphology and distinguish cell types B. What is the difference between Gram-positive
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perfringens Gram stain * Corynebacterium diphtheriae Gram stain * Corynebacterium pseudotuberculosis on blood agar * Enterococcus faecalis * Enterococcus faecalis on blood agar * Enterococcus faecalis Gram stain * Enterococcus faecalis SEM * Erysipelothrix rhusiopathiae on Columbia horse blood agar * Listeria monocytogenes on blood agar * MRSA on Brilliance MRSA Chromogenic Agar (Methicillin-resistantStaphylococcus aureus) * Peptostreptococcus anaerobius Gram stain * Staphylococcus
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Bacillus Cereus Bacillus cereus is a rod-shaped gram- positive bacillus that can be found in food‚ dust‚ dirt and sometimes soil. It is an aerobe and a facultative anaerobe that can form spores‚ which if activated at any point can begin to germinate and produce toxins. These spores that form from B. cereus are be resistant to heat and chemicals. B. cereus is found in approximately 25% of food sampled (3). Foods that are commonly associated with B. cereus are potatoes‚ rice‚ meat‚ vegetables
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Bacillus anthracis in pure culture and injected animals with the bacteria. He then observed that the microbe injected into the animals caused anthrax. Koch was also able to determine the life cycle of Bacillus anthracis. It consists of vegetative and endospore morphologies which alternate based on nutrient availability. There is evidence which shows that the bacteria’s ability to cause anthrax depends on its ability to complete its life cycle in a regulated manner. Koch’s study showed that there is a causal
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Introduction: The Gram stain is a useful stain for identifying and classifying bacteria. The Gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative. This gram stain technique was discovered by Hans Christina Gram in 1884. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive cells which does stain purple. Bacteria that decolorize easily
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Toxoplasma gondii: A Review of Parasitology‚ Pathology‚ and Epidemiology Toxoplasma gondii: A Review of Parasitology‚ Pathology‚ and Epidemiology Parasites are naturally “clever‚” yet insidious organisms. You may have a parasite inside you right now‚ without even knowing it. An effective
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Gram positive stain is a simple procedure? No‚ because there are many procedure to do and more complex than simple ones and use more than one stain to differentiate cellular components. Discussion The Gram stain procedure uses 3 different stains. These are crystal violet‚ Gram’s iodine‚ and safranin. The cells are first stained with crystal violet‚ then Gram’s iodine. Following a rinse in alcohol‚ to de-colorize the cells‚ the cells are then stained with safranin. The Gram stain procedure separates
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simple stains. Crystal violet and Carbol fuchsine‚ simple staining components‚ were used to stain the slide in order to see the different microbes in order to determine their cellular shape and identify unknown ones by comparing. Introduction Bacterial cells are usually colorless because cytoplasm‚ for the most part‚ is transparent. Since the bacteria are colorless‚ it is almost essential to add a stain to make the bacteria more visible. Once stained‚ cell morphology can be observed. Stains are
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Module 16 Blood Stain Patterns Blood Volume 8% of body weight 4 – 5 liters female 5 – 6 liters male 40 % blood loss usually causes death 1.5 liter blood loss can incapacitate Blood Stain Evidence Biblical murderer Cain Abels blood on the ground cried out Cains guilt Abel is the first documented murder Cain is the first documented murderer victim The bloodstain patterns left by falling‚ projected or smeared blood can be analysed by trained crime scene investigators. Careful observation
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