In the years 1928-1941 Stain started a series of Five year plans for industry‚ how far were these successful? Once in power‚ Stalin was determined to modernise the USSR as quickly as possible‚ he wanted to compete with other foreign countries‚ but also he was afraid of a German invasion hence forth he set about achieving modernisation through a series of five year plans. These plans were set up by the GOSPLAN‚ the state planning organisation which Lenin had set up in 1921. The GOSPLAN set ambitious
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offers much more than amusement for its audience. In fact‚ insight and wisdom is often gained from reading‚ which can be applied to most all of our lives in some way. The Case for Christ by Lee Strobel as well as Keeping Faith by Frank and John Schaeffer significantly opposes Holmes conception. Lee Strobel‚ a well know journalist‚ set out to prove the theory of Christianity to be wrong. Throughout his journey‚ he became just the opposite‚ a devoted Christian and firm believer in the Lord. Strobel
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Choice 1. Which of the following stains is used frequently to identify Mycobacterium and other bacteria whose cell walls contain high amounts of lipids? Gram stain Schaeffer-Fulton stain Acid-fast stain Lipid stain Spore Stain 2. Which of the following stains is used to classify microorganisms based on their cell wall content? Capsular stain Gram stain Spore stain Negative stain Methylene blue 3. In the Gram stain‚ Gram-positive bacteria stain with a _________ color due to the incorporation
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Microbiology Lab Quiz #2 Study Guide Exercise 9 -- The Gram Stain Compare and contrast simple and differential staining techniques. Simple Staining Simple staining is useful in determining the basic morphology of an organism. Simple staining involves only one reagent i.e. crystal violet‚ basic fuchsin‚ or methylene blue. Simple dyes used to stain bacteria have a positive charge cationic (+)‚ therefore‚ they are attracted to the bacteria that are usually negatively charged anionic (-) Depends
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Tests done Test | Purpose | Reagents | Result (Positive) | Result (Negative) | Gram Stain | 1. To determine the cell wall formation ( thick layer peptidoglycan or thin layer peptidoglycan) | Crystal violet‚ iodine‚ 95% Alcohol‚ Safranin | Purple specimen- Gram Positive‚ cell wall has thick peptidoglycan layer | Pink Specimen- Gram Negative
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LAB REPORT NUMBER TWO DATE: 3/25/2010 inal attachment Lab Experiment number 11 PURPOSE: To learn the Gram stain technique‚ the reason for the stain‚ and how to identify the results of the organisms stained. MATERIALS: Bunsen burner‚ inoculating loop‚ staining tray‚ glass slides‚ bibulous paper‚ lens paper‚ oil‚ and microscope METHODS: Apply Crystal Violet (Primary stain) for 1 minute. Rinse with D-water Apply Iodine (Mordant) for 1 minute. Rinse with D-water. Apply Alcohol (Decolorize) for
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elements in a specimen is a general term that can refer to a number of specific processes. Using multiple stains can better differentiate between different microorganisms or cellular components of a single organism. Gram’s Stain is a widely used method of staining bacteria as an aid to their identification. It was originally devised by Hans Christian Joachim Gram‚ a Danish physician. Gram’s Stain differentiates between two major cell wall types. Bacterial species with walls containing small amounts
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BTN 211 TOTORIAL 1 Chapter 1 1. The History and Scope of Microbiology Which of the following distinguish the field of microbiology from other fields of biology? a. the size of the organism studied b. the techniques used to study organisms regardless of their size c. both the size of the organism studied and the techniques employed in the study of organisms d. neither the size of the organism studied nor the techniques employed in the study of organisms regardless of their size Who of
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unknown 45. A Gram stain‚ used to indicate the type of cell wall possessed by the bacterium‚ was performed. U45 stained a bright pink color and were small ovals and rods in shape. This indicated that U45 was a gram-negative bacillus bacterium. A capsular stain was then performed to determine the presence or absence of a capsule. Unknown 45 was positive for capsules. Having determined that U45 was gram-negative‚ there was no need to perform the Schaeffer-Fulton endospore stain‚ which is used detect
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Review all chapters in your exercise manual beginning with the introduction INTRODUCTION: (Covered 9.4.14 II Week 1) Biosafety levels1: basic level of containment. Hand washing or wearing gloves 2: Appropriate for working with human body fluids. Autoclave‚ sharps containers‚ lab coats 3: appropriate for working with pathogens that can be transmitted via respiratory route. Self-closing‚ double doors and sealed windows 4: Highest level. Aerosol pathogens; pathogens with no vaccine/treatment. Separate
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