Abstract This experiment was carried out to perform staining methods on microbes‚ explain the mechanisms of staining‚ namely‚ simple staining‚ and learn how to use the microscope. The experiment setting was kept as sterile as possible when conducting the experiment. The microbes under the microscope were drawn out and labelled. In conclusion‚ different staining techniques are used to determine the presence of certain exterior structures of the bacteria like the cell envelope or just to identify
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internal structures such as vacuoles‚ cell walls and spores in details (Seeley and others 1991). Some staining techniques such as Gram staining‚ endospore stain and capsule stain are some of the theories of stains used in bacteriology today. Also‚ these staining procedures help in determining properties of an unknown culture. Gram staining is a differential stain that helps in distinguishing between types of bacteria and it is the most useful staining procedure used today (Seeley and others 1991). In
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differential stain and a special stain (how are they different‚ don’t simply identify the types of differential stains and special stains). What is the role of the Gram’s iodine in a Gram stain? What color would all bacteria become at the end of the staining procedure if the iodine in the gram step was omitted? What is the role of the alcohol wash in the Gram Stain? What color would all bacteria become at the end of the staining procedure if the alcohol step in the gram stain was omitted
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Staining? Cell staining is a technique that can be used to better visualize cells and cell components under a microscope. By using different stains‚ one can preferentially stain certain cell components‚ such as a nucleus or a cell wall‚ or the entire cell. Most stains can be used on fixed‚ or non-living cells‚ while only some can be used on living cells; some stains can be used on either living is or non-living What Cellularcells. Staining? The most basic reason that cells are stained is to enhance visualization
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Gram-positive bacteria with crystal violet. The cell wall of Gram-positive bacteria is made up of a thick layer of peptidoglycan which undergoes dehydration during decolourisation causing the pores to shrink. This shrinkage of pores traps CV-I complex and stain the cell purple. 2. Using a flow chart‚ outline the process of gram staining. (5 marks) Flood the slide with crystal violet for 1 minute then rinse with water Flood the slide with iodine for 1 minute then rinse with water Flood the slide
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observations were performed on four different microscope slides. Three of these observations were gram stains and the last observation was of an endospore staining. The unknown bacteria samples for the endospore stain were taken off of a nutrient agar plate and stained using the Bartholomew and Mittwer’s method. Gram stain samples were taken off of tryptic soy agar and stained using the gram stain procedure that is listed in the Willgoh and Bleakly manual (2). Physiological Tests All biochemical
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that does not bind. C) the colored portion to the dye. D) part of the dye that is not seen. E) the part of the dye that is not used up.4.Which type of staining method would you use to determine endospore-forming cells from non-endospore-forming cells?A) regular stain B) simple stain C) differential stain D) specialized stain5.An acidic dye is:A) a dye the carries no charge. B) a dye the carries a negative charged chromophore. C) a dye that carries no chromophore. D) a dye that carries a positive
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#3. Name the types of microscopes. Simple Microscopes Compound Microscopes Scanning Electron Microscope Transmission Electron Microscope #4. Identify these stains: (Functions and Reagents) Simple Stain- CULTURES: Staphylococcus epidermis slant Bacillus megaterium broth MATERIALS USED: Methylene blue‚ Distilled water‚ Slide‚ Inoculating Loop FUNCTION: To observe shape size‚ morphology‚ and arrangement. ---FROM SOLID: (slant) 1. Mark the smears on the underside
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Bacteria A were translucent in color‚ circular in form‚ with entire margins and a smooth surface. Colonies of Bacteria B were cream colored‚ irregular in form‚ with undulate margins and an umbonate surface. Two different morphologies allowed Gram stains to be performed on each of the bacterium.
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and food products may become contaminated‚ leading to the transient colonization of the human intestine. Illness associated with B. cereus can occur when heat-resistant B. cereus endospores survive cooking. If the food is then inadequately refrigerated or held for extended periods at improper temperatures the endospores can germinate and multiply. Once the spores germinate‚ the vegetative cells can multiply and produce illness causing enterotoxins. Bacillus cereus is known to cause two distinctly
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