One specifically important stain preformed was the Ex 3-7 Gram Stain‚ which showed the EI to be a gram-positive cocci that appeared in small clusters‚ in addition the endospore stain revealed this to be a non-endospore forming bacterial species. These results are important because they drastically narrow the possible bacterial species the EI could be. Many differential
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Review Sheet Exercise I: Survey of Higher Microorganisms: Protozoa‚ Fungi‚ and Helminths Protozoa (group of Kingdom Protista) 1. Amoeba a. nucleus- dark center of the cell b. food vacuole- They feed by taking nutrients into the cell by diffusion and packaging it into (clear circles spread throughout the cell) c. pseudopod- “false foot”; the motility results from the streaming of the protoplasm that forms the process 2. Entamoeba causes amoebiasis or amoebic dysentery‚
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pinkish in color. The first test we did was basic stain using a heat fixed emulsion which kills the bacteria and allows them to adhere to the slide and thickens their protein for better staining. I then covered the heat fixed emulsion with crystal violet for one minute. The stain was then rinsed with distilled water. The stain was then pat dry with bibulous paper then observed under oil immersion to determine morphology. Upon completion of the basic stain organism appeared to be cocci that was in a line
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Microbiology Lecture Exam #1 Outline Chapter 1: Microbiology Introduction I. Microbiology Definition II. History of Microbiology (Christian Gram‚ Robert Koch‚ Antony van Leeuwenhoek‚ Robert Hook‚ Edward Jenner‚ Louis Pasteur‚ Elie Metchnikoff‚ Dmitri Iwanowski‚ Paul Ehrlich‚ Alexander Fleming) II. Scope of Microbiology A. Infectious Agents 1. Prions 2. Viroids 3. Viruses B. Prokaryotes
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Name Microbiology 2210 Answer Key Practice Test 1 Directions: Choose the most correct response to answer the statement. ___1. Archaea a. belong to the kingdom Protista b. have petidoglycan in their cell walls c. belong to the domain Bacteria
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REVISION COMMON TYPES OF MICROBES 1 BACTERIA Difference between Gram Positive and Gram Negative Shapes of bacteria and examples What shapes have you learnt? GRAM STAIN: The process! You should work on your lab notes. LECTURE 2 GRAM STAIN Developed by a Danish called Christian Gram in 1884 Stain a heat-fixed smear with a dye like crystal violet and fix with Iodine Then wash with ethanol or acetone Finally counterstain with a dye of different colour such as safranin (what is its colour
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glass slide. Set the slide to air dry. Either heat fix or methanol fix. (do not fix it both ways) Stain the slides. Properly dispose of any wastes.(waste methanol‚ materials contaminated with bacteria‚ broken glass) Part II. Atlas Sections In your Lab Atlas you will need to read Section 5: Bacterial Cellular Morphology and Simple Stains and Section 6: Bacterial Cell Structures and Differential Stains and then answer the following questions: 2. What is the third important feature of microscopy?
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particular order. Name this Procedure Identify the two types of bacteria present by shape and gram stain. In a gram stain what is the primary stain? The mordant? The counterstain? How does this differ from a simple stain? Identify the shape and gram stain of the bacteria present in this gram stain. Identify the shape and gram stain of the bacteria pictured. Think on this one! Name the gram stain and shape of the bacteria pictured. Name the genus of the organism pictured. Note the size when
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specifically called ________. Question 11 2 out of 2 points A bacterial genus that has waxy my colic acid in the cell walls is ________. Question 12 2 out of 2 points The function of bacterial endospores is ________. Question 13 2 out of 2 points Endospores are ________. Question 14 2 out of 2 points Which of the following is not true of the outer membrane? Question 15 0 out of 2 points A bacterial cell exhibiting chemo taxis probably has ________
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When gram stained gram-negative bacteria stains pink or red; it is colorless and requires safranin as a counterstain. The cell wall of the bacterial cell is a complex‚ semirigid structure responsible for the shape of the cell (Tortora‚ Funke & Case‚ 2013). Gram positive bacteria occupy a multilayered
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