Harley−Prescott: Laboratory Exercises in Microbiology‚ Fifth Edition Front Matter Preface © The McGraw−Hill Companies‚ 2002 PREFACE Take interest‚ I implore you‚ in those sacred dwellings which one designates by the expressive term: laboratories. Demand that they be multiplied‚ that they be adorned. These are the temples of the future—temples of well-being and of happiness. There it is that humanity grows greater‚ stronger‚ better. Louis Pasteur (French chemist‚ founder of microbiology
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destruction due to rupture (Tortora‚ 2010‚ pg 85). Mycoplasma are gram positive when stained. To prepare the sample for staining and stain the sample the following steps would be taken: Take a small sample of sputum and smear it on a slide. This is then tempered by heat on to the slide‚ which will prevent washing it off with the stains. Crystal violet‚ a primary stain‚ is placed
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cyanobacteria and purple and green phototrophic bacteria is? a) energy source b) cell wall type c) Oxygen production d) cell type e) color 17) You have isolated a gram-positive rod. What test will you perform next? a) gram stain b) lactose fermentation c) endospore stain d) flagella stain e) DNA fingerprint Short answers. Fill in the blanks or give a short answer for the following. 1) Your field of view through a microscope is 0.3 millimeters. If you have a worm that takes up half of your field
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test conducted on unknown bacteria 32 was the Gram stain. From this stain‚ unknown 32 was found to be a Gram-positive cocci. This test eliminated all possible Gram-negative bacteria‚ Gram-positive rods and Gram-positive spirillium. Next‚ the endospore test determined whether or not the Gram-positive bacteria contained endospores. With the use of malachite green‚ steam‚ and safranin it was found that unknown bacteria 32 did not contain endospores. This eliminated Gram-positive cocci Sporosarcina spp
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allowed entry of air‚ and yet excluded ________. | | oxygen | | | nutrients | | | airborne bacteria | | | broth | | | heat | Question 4 | | 1 / 1 point | Pasteurization was first developed to kill ________ in wine. | | endospore-producing bacteria | | | all bacteria | | | disease-causing bacteria | | | antibiotic-producing bacteria | | | spoilage bacteria | Question 5 | | 1 / 1 point | Robert Koch’s studies on Bacillus anthracis established a sequence
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` Study Guide for Exam 1 Chapter 1: 1. What is a “microbe”? What groups of organisms are included in this category? Microbes (microorganisms) are minute living things that individually are too small to be seen with the unaided eye the group includes bacteria‚ fungi‚ protozoa‚ and microscopic algae‚ viruses Only minority of microorganisms are pathogenic(disease-producing) 2. What are some of the benefits provided by microbes? Decompose organic waste‚ producers in the ecosystem
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____ 3. Unlike bacteria‚ archaea a.|have cell walls.| b.|are prokaryotes.| c.|have two plasma membranes.| d.|are thought to be closely related to eukaryotes.| ____ 4. Bacteria that cause disease are called a.|viruses.| b.|pathogens.| c.|endospores.| d.|antibiotics.| ____ 5. Where are you likely to find a photoautotroph? a.|in your refrigerator| b.|in the darkness of the ocean| c.|in your digestive system| d.|near the surfaces of lakes‚ streams‚ and oceans| ____ 6. A method
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Isolation of Pure Cultures by Dilution Techniques and Gram Staining Method Results Table 1. Gram stain reaction and cellular features of the culture. Gram staining methods were applied on the given mixture of Bacillus cereus‚ Escherichia coli and Staphylococcus aureus and then examined microscopically. Results were recorded in Table 1. Gram Reactions Cell shapes Cell Ends and Arrangement Size Distinctive Characters Predicted Bacteria Bacteria 1 Gram positive (purple) Cocci Rounded‚ clusters
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When I was done‚ I stored them in the refrigerator for future use. Observations/ Data Tables: Bacteria 40x 100x L. acidophilus (wet mount) Unclear Round cells L. acidophilus (direct stain) Small circles Large blue/purple round cells S. epidermidis (wet mount) Unclear Very small dots S. epidermidis (direct stain) Small dots Dots clumped into one group Results/Analysis: Gained knowledge about culture media and how to distinguish various types of microbial growth. I also learn about variable conditions
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Introduction: The purpose of this lab is to use staining techniques and biochemical testing to identify an unknown bacteria using Bergey’s manual. Bergey’s manual of Systematic Bacteriology is a dichotomous key primarily used to identify a bacterial species. Biochemical tests are used to differentiate different species of bacteria. These tests are effective in determining the characteristics of the microbe being tested. Such characteristics include citrate utilization‚ gelatin hydrolysis‚ nitrate
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