__________________________________ AP Biology LAB 2. ENZYME CATALYSIS ESSAY 2000 The effects of pH and temperature were studied for an enzyme-catalyzed reaction. The following results were obtained. a. How do (1) temperature and (2) pH affect the activity of this enzyme? In your answer‚ include a discussion of the relationship between the structure and the function of this enzyme‚ as well as a discussion of how structure and function of enzymes are affected by temperature and pH. b. Describe
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Biology “Enzyme Activities” Introduction: Enzymes have extremely interesting properties that make them little chemical-reaction machines. The purpose of an enzyme in a cell is to allow the cell to carry out chemical reactions very quickly. These reactions allow the cell to build things or take things apart as needed. This is how a cell grows and reproduces. At the most basic level‚ a cell is really a little bag full of chemical reactions that are made possible by enzymes (Brain). Laboratory
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it’s lowest to do so. However‚ in cold solutions the starch will take longer as it will in temperatures beyond 40 degrees. Once it reaches this point‚ the break down will either take a very long period of time or have no reaction at all as enzymes are denatured at a certain point. Materials: · 4 x test tubes · 5mL Diastase · 5mL Water · 10mL 2% Starch Suspension. · Pipette · 2 x Spotting tiles · Large Beaker filled with water of assigned temperature · Thermometer
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Enzyme as protein Dr.Samina Haq Quantitative and qualitative test for protein and amino acids • 1. 2. 3. 4. 5. 6. Qualitative test Ninhydrin test Biuret test Xanthoproteic test Millons test Hopkins-cole test Nitroprusside test Quantitative test 1. 2. 3. Spectrophotometric assay Protein shows maximum absorbance at 280nm due to presence of tyrosine and tryptophane. Biuret test shows 540nm Lowry test shows 750nm Ninhydrin Test • Amino acid containing a free
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amylase and starch. Introduction The enzyme amylase is found in the human body‚ it catalyses the hydrolosis of internal glycosidic bonds in polysaccharides‚ the breakdown of starch into sugars. Amylase is present in human saliva‚ where it initiates the chemical process of digestion. Enzymes work best at an optimum pH of 7 which is the bodies normal pH. The pH affects the charge of the amino acid at the active site. PH changes affect the structure of an enzyme molecule and therefore affect its ability
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Observing Flames and Specs through a Spectroscope By: Seif Alkhouri Cristian Plancia-Mata Charlotte Maxwell Suryansh Kumar Evan Hatton J. Immel Ph.D Introduction Spectroscopy is the study of the interaction between matter and radiated energy. English philosopher Roger Bacon(1214-1294) was the first person to recognize that if sunlight passes through water it splits into colors. Around four centuries ago‚ Isaac Newton originated the title “spectrum” to label the shade of rainbows put
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Enzymes (pron.: /ˈɛnzaɪmz/) are large biological molecules responsible for the thousands of chemical interconversions that sustain life.[1][2] They are highly selective catalysts‚ greatly accelerating both the rate and specificity of metabolic reactions‚ from the digestion of food to the synthesis of DNA. Most enzymes are proteins‚ although some catalytic RNA molecules have been identified. Enzymes adopt a specific three-dimensional structure‚ and may employ organic (e.g. biotin) and inorganic (e
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Scientific Breakthrough Paper Forensic DNA typing has had a broad‚ positive impact on the criminal justice system. In recent years‚ convictions have been obtained that previously would have been impossible. Countless suspects have been eliminated prior to the filing of charges. Old‚ unsolved criminal cases‚ as well as new cases‚ have been solved. In a very few case‚ mistakenly accused defendants have been freed both before trial and after incarceration. Increasingly‚ the unidentified remains of
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There are approximately 40‚000 enzymes living in one human cell‚ each responsible for a chemical reaction. Enzymes are complex 3D protein molecules created by amino acids‚ forming a unique sequence that produces hydrogen bonds‚ eventually formulating an enzyme within plants and animals (Boyle & Senior‚ 2002). Working alongside other molecules‚ they uphold a stable reaction system. The function of an enzyme is to aid and increase chemical reactions and organise metabolism‚ while maintaining homeostasis
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milk Introduction Enzymes are globular protein‚ responsible for most of the chemical activities of living organisms. They are made up of long chains of amino acids containing carbon‚ hydrogen‚ oxygen and nitrogen (Gunsch‚ 2012). The role of enzyme is to act as catalysts‚ substances that speed up chemical reactions without being chemically altered during the process. The speeding up of chemical reactions is done by lowering the activation energy required to start a reaction. Enzymes are specific in
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