Use of SDS-PAGE/Western Blot Techniques as Diagnostic Tools for Equine Medical Conditions SDS-PAGE Overview Electrophoresis is commonly used to separate proteins using an applied electrical field. SDS-PAGE separates proteins by molecular weight‚ using sodium dodecyl sulfate (SDS; also known as lauryl sulfate) to denature them and a discontinuous polyacrylamide gel as a sieving matrix. The net charges of folded proteins are not dependent upon molecular weight. Rather‚ charge is determined by
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this goal by creating five reactions containing Sodium Sulfate that would confirm our compound and show its chemical properties. In each reaction‚ we replaced the presence of Sodium Sulfate with our unknown. Our first reaction was the reaction from the sulfate anion test between Sodium Sulfate and Barium Chloride. If the compound was in fact Sodium Sulfate it would produce a white precipitate and it did. The second reaction was first between Sodium Sulfate and Hydrochloric Acid‚ and then Silver Nitrate
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What is gel electrophoresis? Gel electrophoresis is a technique that separates pieces of DNA (or other biological molecules) by size. Pieces of DNA in a test tube all look the same. DNA Gel Electrophoresis Gel electrophoresis separates pieces of DNA by size so that researchers can further analyze them BURST Training Session November 29‚ 2005 Once the DNA samples are loaded onto the gel‚ an electric current is applied to the gel. DNA is negatively charged due to all the phosphate groups in the
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J Comput Virol (2006) 2:67–77 DOI 10.1007/s11416-006-0012-2 ORIGINAL PAPER Dynamic analysis of malicious code Ulrich Bayer · Andreas Moser · Christopher Kruegel · Engin Kirda Received: 13 January 2006 / Accepted: 27 March 2006 / Published online: 16 May 2006 © Springer-Verlag France 2006 Abstract Malware analysis is the process of determining the purpose and functionality of a given malware sample (such as a virus‚ worm‚ or Trojan horse). This process is a necessary step to be
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The Spectrophotometer Determination Of Protein Concentrations And The Effects Sodium Dodecyl Sulphate And Triton X-100 Have On Protein Concentration. INTRODUCTION Spectroscopy is used as a collective term for all the analytical techniques based on the interaction of light and matter. Spectrophotometry is one of the branches of spectroscopy where we measure the absorption of light by molecules that are in a gas or vapour state or dissolved molecules/ions (Tombs‚ et.al‚ 1959). Spectroscopy is the
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SODIUM gSodium is a chemical element with the symbol Na (from Latin: natrium) and atomic number 11. It is a soft‚ silver-white‚ highly reactive metal and is a member of the alkali metals; its only stable isotope is 23Na. The free metal does not occur in nature‚ but instead must be prepared from its compounds; it was first isolated by Humphry Davy in 1807 by the electrolysis of sodium hydroxide. Sodium is the sixth most abundant element in the Earth’s crust‚ and exists in numerous minerals such
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Making an Agarose Gel AIM: To be able to make and agarose gel and perform gel electrophoresis in six different dyes. Also‚ to extract DNA from wheat germ. INTRODUCTION: Agarose gel is a substance that is used in science for gel electrophoresis and size exclusion chromatography. These processes use agarose gel to separate and analyze proteins and DNA. The medium is composed of a purified agarose powder that has been boiled in a buffer solution and then cooled into a gel. Agarose gel is most commonly
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GRAVIMETRIC DETERMINATION OF SULFATE Purpose You will be given a solid powder‚ which has been dried to constant mass. The sample is water soluble. The purpose of this experiment is to determine the concentration of sulfate in your solid sample using gravimetric analysis. Background Gravimetric Analysis The goal of most quantitative chemical analysis measurements is to estimate the relative abundance of an analyte in a chemical sample. For solid and liquid samples‚ a very common expression
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Sodium From Wikipedia‚ the free encyclopedia This article is about the chemical element. For the PlayStation Home game‚ see Sodium (PlayStation Home). For the racehorse‚ seeSodium (horse). "Natrium" redirects here. For the town in West Virginia‚ see Natrium‚ West Virginia. Sodium 11Na
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subject: Serum protein electrophoresis. Report No.: 1. Serum Protein Electrophoresis (SPEP) Introduction: The serum protein electrophoresis (SPEP) test measures specific proteins in the blood to help identify some diseases. And its uses an electrical field to separate the proteins in the blood serum into groups of similar size‚ shape‚ and charge. And here we’ll use gel electrophoresis which indicate that blood serum is placed on special paper treated with agarose gel and exposed to an electric
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