Roy Levin Bio 11 Lab Dr.Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to
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plus distilled water and air as the control. When performing this experiment‚ the main element to consider is hypertonic‚ hypotonic and isotonic solutions and how they relate to osmosis. Osmosis is the process where a fluid passes through a semipermeable membrane‚ which moves from an area which the solute is present in high concentrations. As a result of osmosis‚ there will be an equal amount of fluid on either side of the barrier‚ creating an isotonic solution. When cells are placed in an isotonic
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Membrane Processing MEMBRANEFILTRATION A membrane or‚ more properly‚ a semipermeable membrane‚ is a thin layer of material capable of separating substances when a driving force is applied across the membrane. Membrane filtration enables to separate particles with a diameter smaller than the pore diameter in the membrane from the liquid feed‚ by applying a driving force (pressure) over the membrane. The membrane technology consists principally of Microfiltration (MF)‚ Ultrafiltration
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Wave Nature of Light Objective: The purpose of this lab is to investigate interference‚ otherwise known as the diffraction of light. A beam of light acts a wave‚ and we are able to use equations so calculate the wavelength of the light used. The diffraction of a straight edge demonstrates that light waves bend around straight edges‚ allowing light to enter an area of shadow. When waves are superposed‚ they reinforce each other when crests are in phase and cancel out when they are not in phase
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PEES 4200W- Physiology of Exercise Lab #2: Metabolism and Energy Expenditure 1. The Respiratory exchange ratio is the ratio of the volume of carbon dioxide produced and oxygen consumed. The ratio indicates the energy that the subject is expending for indirect calorimetry‚ how efficient the subject ’s body is at utilizing the oxygen inhaled‚ as well as the main substrate being used for energy during varying intensities of exercise. During rest the volume of carbon dioxide was 0.73L/min‚ the volume
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THE EFFECT OF THE WATER CONCENTRATION GRADIENT ON THE RATE OF OSMOSIS Investigation Folio: Summative Stage 1 Biology: SEMESTER 1 2014 MARCIA SHAW - 151003X INTRODUCTION Diffusion: is when something spreads more widely from a higher concentration gradient to a lower concentration gradient. Diffusion is the process from which molecules‚ atoms or ions mingle together and move from an area of high concentration to an area of low concentration as a result to their kinetic energy. for example
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way through the lab material typing in your answers. Then submit your completed lab report through the “Assignment” feature. This lab report is worth 50 points towards your final lab grade. Also‚ per the Honor Code‚ this work must be your own. CELLS Using your Biology Textbook answer the following questions. 1. What types of organisms are Prokaryotes? The Prokaryotes are organisms that are single-celled‚ and their DNA is not confined to a nucleus and do not have membrane bounded organelles
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chemicals before use. Abide by specific warnings and directions. 3. Collect all materials needed for a procedure before proceeding. 4. Perform reactions under the hood when directed. Chemicals may be weighed and prepared at balance or lab tables‚ but tests should be carried out under the hood. 5.Acids and caustic chemicals are stored in the hood. Please do not take these chemicals from the hood. Procedure: PART 1: Metathetical reactions Precipitation reactions A1. Add a
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Organic Chemistry II Lab 9 Fermentation of a Carbohydrate: Ethanol from Sucrose * Introduction Ethanol is one of the oldest alcohols and also the least toxic one. Industrially‚ ethanol is made most economically by hydration of ethylene. However‚ ethanol that is intended for human consumption must‚ by law‚ be prepared by fermentation. By either method‚ ethanol‚ of course‚ has the same formula‚ structure‚ and properties. The fermentation takes place with the assistance of enzymes from yeast
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The cell‚ which is the smallest unit of life‚ is surrounded by a plasma membrane. The plasma membrane functions somewhat like a wall‚ as it keeps the internal contents from the external environment. Just like a wall‚ the membrane is also somewhat permeable‚ except that the membrane takes a much more active role in determining what is allowed in to the cell and what is kept out. The plasma membrane is a very thin structure‚ which has some very important tasks. One of the main tasks that it performs
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